aPCR-SSCP and DNA sequencing detecting two silent SNPs at KAP8.1 gene in the cashmere goat

Abstract

Keratin-associated proteins 8.1 gene (KAP8.1) is a structural gene responsible for the cashmere. KAP8.1 protein contains high glycine and tyrosine, which concerns regulation and function of the matrix structure fiber. In this study, the polymorphism of KAP8.1 gene was detected by methods of aPCR-SSCP (asymmetric polymerase chain reaction single-strand conformation polymorphism) and DNA sequencing in 791 individuals from two breeds. The results showed that there were two mutations in this gene. The mutations were described as c.63 T>G and c.66 C>G, which would result in two synonymous mutations in KAP8.1 protein. The findings go against previous research, in which there was not polymorphism at KAP8.1 gene. The reasons might be that different cashmere breeds were detected in two studies. Further analysis of results leads us to believe that the polymorphism of KAP8.1 gene might be relevant to fiber diameter.

Isolation and characteristics of the melanocortin 1 receptor gene (MC1R) in the Chinese yakow (Bos grunniens×Bos taurus)

“…2 and 3. According to the classification of PIC (low polymorphism for a PIC value b0.25, median polymorphism for a PIC value >0.25 and b0.5, and high polymorphism for a PIC value >0.5; Zhao et al, 2009), SNPs G-1A, C201T, T206C, C870T and T890C each had a low polymorphism. The remaining 8 loci from the MC1R had median polymorphic levels.…”

Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)

“…After the wool follicles become mature and active, it undergoes three regular periods termed anagen, catagen and telogen in a yearly cycle [2]. Cashmere characteristics are distinctly different depending on the region and goat species [3]. …”

Expression of the GPRC5D gene in Liaoning Cashmere goats

“…The Liaoning Cashmere goat has a particularly fine, variable Cashmere fleece (12). Cashmere has a very good textile performance, which is closely related to increased cashmere yield (13). Some scholars have studied GPRC5D of the RAIG-1 family in man and rat (3,14).…”