Apelin Receptor Signaling During Mesoderm Development

Sağraç, Derya; Şişli, Hatice Burcu; Doğan, Ayşegül

doi:10.1007/5584_2020_567

Cited by 4 publications

(1 citation statement)

References 108 publications

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“…Previous studies have demonstrated that impaired myofibril assembly can adversely affect heart development (Fritz-Six et al, 2003) and is closely related to congenital heart defects (Granados-Riveron et al, 2010). Moreover, a deficiency in the Apelin signaling pathway has been shown to reduce the number of myocardial progenitor cells and impair heart development (Quertermous, 2007;Sagrac et al, 2020). Additionally, mitochondrial function has been recognized as crucial in myocyte differentiation and heart development (Lingan et al, 2017;Zhao et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Microbiota affects mitochondria and immune cell infiltrations via alternative polyadenylation during postnatal heart development

Liu,

Shao,

Han

et al. 2024

Front. Cell Dev. Biol.

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There is a growing body of evidence supporting the significant impact of microbiota on heart development. Alternative polyadenylation (APA) is a crucial mechanism for gene expression regulation and has been implicated in postnatal heart development. Nonetheless, whether microbiota can influence postnatal heart development through the regulation of APA remains unclear. Therefore, we conducted APA sequencing on heart tissues collected from specific pathogen-free (SPF) mice and germ-free (GF) mice at three different developmental stages: within the first 24 h after birth (P1), 7-day-old SPF mice, and 7-day-old GF mice. This approach allowed us to obtain a comprehensive genome-wide profile of APA sites in the heart tissue samples. In this study, we made a significant observation that GF mice exhibited noticeably longer 3ʹ untranslated region (3ʹ UTR) lengths. Furthermore, we confirmed significant alterations in the 3ʹ UTR lengths of mitochondria-related genes, namely Rala, Timm13, and Uqcc3. Interestingly, the GF condition resulted in a marked decrease in mitochondrial cristae density and a reduction in the level of Tomm20 in postnatal hearts. Moreover, we discovered a connection between Rala and Src, which further implicated their association with other differentially expressed genes (DEGs). Notably, most of the DEGs were significantly downregulated in GF mice, with the exceptions being Thbs1 and Egr1. Importantly, the GF condition demonstrated a correlation with a lower infiltration of immune cells, whereby the levels of resting NK cells, Th17 cells, immature dendritic cells, and plasma cells in GF mice were comparable to those observed in P1 mice. Furthermore, we established significant correlations between these immune cells and Rala as well as the related DEGs. Our findings clearly indicated that microbiota plays a vital role in postnatal heart development by affecting APA switching, mitochondria and immune cell infiltrations.

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Section: Discussionmentioning

confidence: 99%

Microbiota affects mitochondria and immune cell infiltrations via alternative polyadenylation during postnatal heart development

Liu,

Shao,

Han

et al. 2024

Front. Cell Dev. Biol.

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Circulating levels of Elabela in pregnant women with missed abortion

Hou

et al. 2022

Gynecological Endocrinology

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WNT-mediating TCF/LEF transcription factor gene expression in early human pluripotency and cell lineages differs from the rodent paradigm

Ross,

Azami,

Salonna

et al. 2024

Preprint

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Embryonic stem cell research has uncovered different requirements for WNT/beta-catenin signalling in human naive pluripotent cells compared to the mouse paradigm. It is therefore important to study WNT/beta-catenin signalling directly in models of early human development. Since TCF/LEF factors mediate the regulation of target genes downstream of WNT/beta-catenin signalling, we studied the expression and protein localisation of the four TCF/LEF genes by analysing in vitro snapshots of human development, leveraging naive and primed pluripotent cells as well as extraembryonic and early embryonic cell lineages. Strikingly, we comprehensively confirm clear differences between mouse and human pluripotent stem cells, suggesting species-specific requirements for WNT signalling that may reflect differences in states of pluripotency. Human naive ES cells express very low TCF7L1, unlike their mouse counterparts. TCF7L2 is robustly expressed in human naive ES-derived trophectoderm cells. In human primed pluripotent stem cells, activation of WNT/beta-Catenin signalling is required to induce expression of both TCF7 and LEF1, concomitant with hallmark gastrulation markers. This expression of human TCF/LEF genes benchmarks differential requirements for WNT/beta-catenin signalling throughout early human embryo development that requires further investigation.

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Apelin Receptor Signaling During Mesoderm Development

Cited by 4 publications

References 108 publications

Microbiota affects mitochondria and immune cell infiltrations via alternative polyadenylation during postnatal heart development

Microbiota affects mitochondria and immune cell infiltrations via alternative polyadenylation during postnatal heart development

Circulating levels of Elabela in pregnant women with missed abortion

WNT-mediating TCF/LEF transcription factor gene expression in early human pluripotency and cell lineages differs from the rodent paradigm

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