Aperture scanning Fourier ptychographic microscopy

Ou, Xiaoze; Chung, Jaebum; Horstmeyer, Roarke; Yang, Changhuei

doi:10.1364/boe.7.003140

Cited by 43 publications

(29 citation statements)

References 24 publications

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“…Phase information can also be retrieved by using computational algorithms [8]. For example, Fourier ptychographic microscopy (FPM) takes a series of images captured under different illumination angles [9][10][11][12][13] or different overlapped apertures [14][15][16] to iteratively recover a sample's phase and amplitude; transport of intensity phase imaging [17][18][19][20] uses images captured at different defocused planes to solve the transport of intensity equation (TIE) and retrieve phase. The large number of measurements needed is a disadvantage of these methods.…”

Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

Lu¹,

Chung²,

Ou³

et al. 2016

Opt. Express

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Differential phase contrast (DPC) is a non-interferometric quantitative phase imaging method achieved by using an asymmetric imaging procedure. We report a pupil modulation differential phase contrast (PMDPC) imaging method by filtering a sample's Fourier domain with half-circle pupils. A phase gradient image is captured with each half-circle pupil, and a quantitative high resolution phase image is obtained after a deconvolution process with a minimum of two phase gradient images. Here, we introduce PMDPC quantitative phase image reconstruction algorithm and realize it experimentally in a 4f system with an SLM placed at the pupil plane. In our current experimental setup with the numerical aperture of 0.36, we obtain a quantitative phase image with a resolution of 1.73μm after computationally removing system aberrations and refocusing. We also extend the depth of field digitally by 20 times to ±50μm with a resolution of 1.76μm.

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Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

Lu¹,

Chung²,

Ou³

et al. 2016

Opt. Express

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“…Increased resolution/SBP Aperture synthesis [1,[21][22][23][24] Phase imaging Phase retrieval [25][26][27][28][29] Digital refocusing Phase retrieval [1,27,30] Aberration correction EPRY [24,[31][32][33] Long working distance low NA objective [34,35] Sub-λ imaging high-angle illumination [21][22][23]34,36,37] Multimodal imaging scanning illumination angle [24,34,38] High-speed LED & camera multiplexing [26,[39][40][41][42][43][44][45] Compact and portable Novel hardware [33,46,47] 3D imaging light field, 1st Born, multislice [48][49][50][51][52][53][54][55][56] computation using low numerical aperture (NA) op...…”

Section: Achieved By Referencesmentioning

confidence: 99%

“…The maximum synthesized NA that can be expected for an FP system in air is 2 -when NA ill and NA obj are both one. With scanning [27,30,76] and multi-aperture [42] FP setups, the synthetic NA may be defined via a similar process and its extent in Fourier space also defines the system's final resolution.…”

Section: Resolutionmentioning

confidence: 99%

“…FP can also be implemented without LEDs in an aperture-scanning mode, where the aperture is physically moved across the sample's diffracted spectrum while recording images [27,28,30]. This method can apply to thick samples in either transmission or reflection geometry, while phase recovery provides the ability to digitally refocus and observe features at different depths in these 3D samples (Fig.…”

Section: Phase Reconstruction Applicationsmentioning

confidence: 99%

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Fourier ptychography: current applications and future promises

et al. 2020

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Traditional imaging systems exhibit a well-known trade-off between the resolution and the field of view of their captured images. Typical cameras and microscopes can either "zoom in" and image at high-resolution, or they can "zoom out" to see a larger area at lower resolution, but can rarely achieve both effects simultaneously. In this review, we present details about a relatively new procedure termed Fourier ptychography (FP), which addresses the above trade-off to produce gigapixel-scale images without requiring any moving parts. To accomplish this, FP captures multiple low-resolution, large field-of-view images and computationally combines them in the Fourier domain into a high-resolution, large field-of-view result. Here, we present details about the various implementations of FP and highlight its demonstrated advantages to date, such as aberration recovery, phase imaging, and 3D tomographic reconstruction, to name a few. After providing some basics about FP, we list important details for successful experimental implementation, discuss its relationship with other computational imaging techniques, and point to the latest advances in the field while highlighting persisting challenges.

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“…There also have been numerous efforts in improving the Fourier ptychographic (FP) reconstruction by adopting more noise-robust algorithms [15][16][17][18]. Alternative FPM modalities involving aperture scanning instead of angular illuminations were demonstrated, which allowed for imaging the complex field of a thick specimen [19,20] and estimating optical aberrations [21]. Imaging a thick specimen with angular illuminations also became possible by employing the first Born approximation [22] or multislice coherent model [23].…”

Section: Introductionmentioning

confidence: 99%

Wide-field Fourier ptychographic microscopy using laser illumination source

Chung¹,

Lu²,

Ou³

et al. 2016

Biomed. Opt. Express

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Fourier ptychographic (FP) microscopy is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple images drives the need for a brighter illumination scheme and a high-frame-rate camera for a faster acquisition. We report the use of a guided laser beam as an illumination source for an FP microscope. It uses a mirror array and a 2-dimensional scanning Galvo mirror system to provide a sample with plane-wave illuminations at diverse incidence angles. The use of a laser presents speckles in the image capturing process due to reflections between glass surfaces in the system. They appear as slowly varying background fluctuations in the final reconstructed image. We are able to mitigate these artifacts by including a phase image obtained by differential phase contrast (DPC) deconvolution in the FP algorithm. We use a 1-Watt laser configured to provide a collimated beam with 150 mW of power and beam diameter of 1 cm to allow for the total capturing time of 0.96 seconds for 96 raw FPM input images in our system, with the camera sensor's frame rate being the bottleneck for speed. We demonstrate a factor of 4 resolution improvement using a 0.1 NA objective lens over the full camera field-of-view of 2.7 mm by 1.5 mm. References and links1. G. Zheng, R. Horstmeyer, and C. Yang, "Wide-field, high-resolution Fourier ptychographic microscopy," Nat. Photonics 7(9), 739-745 (2013). 2. X. Ou, R. Horstmeyer, C. Yang, and G. Zheng, "Quantitative phase imaging via Fourier ptychographic microscopy,"Opt. Lett. 38(22), 4845-4848 (2013). 3. R. Hegerl, W. Hoppe, "Dynamic theory of crystal structure analysis by electron diffraction in the inhomogeneous primary radiation wave field," Ber. Bunsenges. Phys. Chem. 74(11), 1148-1154 (1970). 4. X. Ou, R. Horstmeyer, G. Zheng, and C. Yang, "High numerical aperture Fourier ptychography: principle, implementation and characterization," Opt. Express 23(3), 3472-3491 (2015). 5. J. Chung, X. Ou, R. P. Kulkarni, and C. Yang, "Counting White Blood Cells from a Blood Smear Using Fourier Ptychographic Microscopy," PloS ONE 10(7), e0133489 (2015). 6. S. Dong, K. Guo, P. Nanda, R. Shiradkar, and G. Zheng, "FPscope: a field-portable high-resolution microscope using a cellphone lens," Biomed. Opt. Express 5(10), 3305-3310 (2014). 7. K. Guo, Z. Bian, S. Dong, P. Nanda, Y. M. Wang, and G. Zheng, "Microscopy illumination engineering using a low-cost liquid crystal display," Biomed. Ultramicroscopy 109(10), 1256-1262 (2009). 11. X. Ou, G. Zheng, and C. Yang, "Embedded pupil function recovery for Fourier ptychographic microscopy," Opt.Express 22(5), 4960-4972 (2014). 12. A. Williams, J. Chung, X. Ou, G. Zheng, S. Rawal, Z. Ao, R. Datar, C. Yang, and R. Cote, "Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis," J.

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Aperture scanning Fourier ptychographic microscopy

Cited by 43 publications

References 24 publications

Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

Quantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast

Fourier ptychography: current applications and future promises

Wide-field Fourier ptychographic microscopy using laser illumination source

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