APH-1, a POU homeobox gene expressed in the salt gland of the crustacean Artemia franciscana

Chavez, Marcela; Landry, Claire; Loret, Suzanne; Müller, Marc; Figueroa, Jaime; Peers, Bernard; Rentier-Delrue, Françoise; Rousseau, Guy; Krauskopf, Manuel; Martial, Joseph

doi:10.1016/s0925-4773(99)00152-5

Cited by 21 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tracheal cells and spiracles on T2 and T3 arise from locations lateral to the leg bases (23) possibly homologous with the association between gills and appendages in crustaceans, a hypothesis strengthened by the finding that homologs of tracheal inducer genes (trh, vvl) are specifically expressed in the gills of crustaceans (24,41). Notably, those genes were absent from the small T1 ectopic wings of Oncopeltus.…”

Section: Discussionmentioning

confidence: 99%

Origin and diversification of wings: Insights from a neopteran insect

Medved

Marden

Fescemyer

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Winged insects underwent an unparalleled evolutionary radiation, but mechanisms underlying the origin and diversification of wings in basal insects are sparsely known compared with more derived holometabolous insects. In the neopteran species Oncopeltus fasciatus, we manipulated wing specification genes and used RNA-seq to obtain both functional and genomic perspectives. Combined with previous studies, our results suggest the following key steps in wing origin and diversification. First, a set of dorsally derived outgrowths evolved along a number of body segments including the first thoracic segment (T1). Homeotic genes were subsequently co-opted to suppress growth of some dorsal flaps in the thorax and abdomen. In T1 this suppression was accomplished by Sex combs reduced, that when experimentally removed, results in an ectopic T1 flap similar to prothoracic winglets present in fossil hemipteroids and other early insects. Global geneexpression differences in ectopic T1 vs. T2/T3 wings suggest that the transition from flaps to wings required ventrally originating cells, homologous with those in ancestral arthropod gill flaps/epipods, to migrate dorsally and fuse with the dorsal flap tissue thereby bringing new functional gene networks; these presumably enabled the T2/T3 wing's increased size and functionality. Third, "fused" wings became both the wing blade and surrounding regions of the dorsal thorax cuticle, providing tissue for subsequent modifications including wing folding and the fit of folded wings. Finally, Ultrabithorax was co-opted to uncouple the morphology of T2 and T3 wings and to act as a general modifier of hindwings, which in turn governed the subsequent diversification of lineage-specific wing forms.wing origins | Sex combs reduced | Ultrabithorax | RNA-seq | vestigial S ome 350 million years ago, the development of insect wings was a seminal event in the evolution of insect body design (1, 2). The ability to fly was critical to insects becoming the most diverse and abundant animal group, and the origin of such novelty has been a focus of intense scientific inquiry for more than a century (3, 4). More recently, through studies of genetic model systems such as Drosophila, the mechanisms of wing morphogenesis have been elucidated (5-12). Still lacking however is a comprehensive understanding of transitional steps connecting the morphology of structures observed in the fossil record with that of the modern-day insects, including wing origins and subsequent diversification.The initial stages of insect wing evolution are missing from the fossil record and it is therefore necessary to use indirect evidence from fossils that postdate the origin and initial radiation of pterygotes (2). Larvae of many of those taxa featured dorsally positioned outgrowths on each of the thoracic and abdominal segments (2, 13), apparently serial homologs (i.e., similar structures likely arising from a common set of developmental mechanisms). Diverse lineages independently lost those dorsal appendages on the abdomen while un...

show abstract

Section: Discussionmentioning

confidence: 99%

Origin and diversification of wings: Insights from a neopteran insect

Medved

Marden

Fescemyer

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…An antibody against the Vvl protein of Artemia was generated by injecting rabbits with a His-tagged fragment of the Vvl protein [amino acids 241 to 386 of the previously published sequence (Chavez et al, 1999)]. The serum was then affinity purified on a nickel column.…”

Section: Artemia Parhyale and Crayfish Immunostaining And In Situ Hymentioning

confidence: 99%

“…In the case of Artemia vvl, we cloned a fragment that corresponds to the APH-1 gene previously reported by Chavez et al (Chavez et al, 1999) and generated an antibody for immunochemical staining in developing Artemia larvae. We observe that Artemia Vvl is initially absent from early limb buds; it becomes weakly and uniformly expressed while the limb is developing its characteristic branching morphology, and becomes strongly upregulated in one of the epipods as its cells begin to differentiate (Fig.…”

Section: Crustacean Homologues Of Tracheal Inducer Genes Are Expressementioning

confidence: 99%

Association of tracheal placodes with leg primordia inDrosophilaand implications for the origin of insect tracheal systems

et al. 2006

View full text Add to dashboard Cite

Adaptation to diverse habitats has prompted the development of distinct organs in different animals to better exploit their living conditions. This is the case for the respiratory organs of arthropods, ranging from tracheae in terrestrial insects to gills in aquatic crustaceans. Although Drosophila tracheal development has been studied extensively, the origin of the tracheal system has been a long-standing mystery. Here, we show that tracheal placodes and leg primordia arise from a common pool of cells in Drosophila, with differences in their fate controlled by the activation state of the wingless signalling pathway. We have also been able to elucidate early events that trigger leg specification and to show that cryptic appendage primordia are associated with the tracheal placodes even in abdominal segments. The association between tracheal and appendage primordia in Drosophila is reminiscent of the association between gills and appendages in crustaceans. This similarity is strengthened by the finding that homologues of tracheal inducer genes are specifically expressed in the gills of crustaceans. We conclude that crustacean gills and insect tracheae share a number of features that raise the possibility of an evolutionary relationship between these structures. We propose an evolutionary scenario that accommodates the available data.

show abstract

“…In situ hybridization as a means to detect the RNA expression in cells and tissues has been widely described for D. rerio, Artemia, Drosophila and other species (Chavez et al, 1999;Mitchell and Crews, 2002;Thisse and Thisse, 2007), but studies on small cladocerans are much less common. In this study, we analyzed the spatial expression sites of DapcaDsx2 to ensure the integrity of D. carinata individuals by using digoxigenin-labeled RNA probes for the first time.…”

Section: Discussionmentioning

confidence: 99%

“…The whole-mount in situ hybridization technique employed the methods used by Thisse et al (Chavez et al, 1999;Mitchell and Crews, 2002;Thisse and Thisse, 2007), with changes based on the characteristics of D. carinata. Individual specimens were digested with proteinase K for about 15 min.…”

Section: Whole-mount In Situ Hybridizationmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages

Zhang¹,

A²,

Lv³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in D. magna, D. pulex, and D. galeata respectively. Phylogenetic analysis revealed that DapcaDsx2 clustered in the same class, and was evolutionarily more distant to sequences from other species. qRT-PCR showed that DapcaDsx2 was most abundantly expressed during sexual reproduction (P < 0.05). Using digoxigenin-labeled RNA probes, we studied DapcaDsx2 expression in parthenogenetic and sexual females by whole-mount in situ hybridization. The results revealed that DapcaDsx2 was mainly Cloning and expression of Doublesex in D. carinata expressed in the second antennae and several sites of the ventral carapace, whereas higher expression levels were found in sexual than in parthenogenetic females. This suggests that the DapcaDsx2 gene is involved in switching modes of reproduction and in sexual differentiation.

show abstract

APH-1, a POU homeobox gene expressed in the salt gland of the crustacean Artemia franciscana

Cited by 21 publications

References 18 publications

Origin and diversification of wings: Insights from a neopteran insect

Origin and diversification of wings: Insights from a neopteran insect

Association of tracheal placodes with leg primordia inDrosophilaand implications for the origin of insect tracheal systems

Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages

Contact Info

Product

Resources

About