Aphid Odorant-Binding Protein 9 Is Narrowly Tuned to Linear Alcohols and Aldehydes of Sixteen Carbon Atoms

D’Onofrio, Chiara; Knoll, Wolfgang; Pelosi, Paolo

doi:10.3390/insects12080741

Cited by 11 publications

(5 citation statements)

References 42 publications

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“…FCBA result showed that GOBP with broad spectrum, such as CsasGOBP1–2 displayed high binding affinity to general components of plant volatiles [ 31 ] and CpunGOBP2 displaying the widest binding spectra [ 23 ]. Furthermore, ApisOBP9 shows high and specific affinity to linear alcohols and aldehydes of 16-carbon [ 48 ] and EoblOBP6 binds the tea plant volatile, benzaldehyde and display high binding ability to herbivore-induced plant volatiles (HIPVs), nerolidol and α-farnesene [ 49 ] with the narrow spectrum. Similarity, in our previous studies, FCBA result showed that EsigGOBP1 mainly bind benzene, 1,2-diethyl-, alpha-phellandrene, butyl acrylate and camphene.…”

Section: Discussionmentioning

confidence: 99%

EsigGOBP1: The Key Protein Binding Alpha-Phellandrene in Endoclita signifer Larvae

Hao

Zhende

et al. 2022

IJMS

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Endoclita signifer larvae show olfactory recognition towards volatiles of eucalyptus trunks and humus soils. Further, EsigGOBP1 was identified through larval head transcriptome and speculated as the main odorant-binding proteins in E. signifer larvae. In this study, the highest expression of EsigGOBP1 was only expressed in the heads of 3rd instar larvae of E. signifer, compared with the thorax and abdomen; this was consistent with the phenomenon of habitat transfer of 3rd instar larvae, indicating that EsigGOBP1 was a key OBP gene in E. signifer larvae. Results of fluorescence competition binding assays (FCBA) showed that EsigGOBP1 had high binding affinities to eight GC-EAD active ligands. Furthermore, screening of key active odorants for EsigGOBP1 and molecular docking analysis, indicated that EsigGOBP1 showed high binding activity to alpha-phellandrene in 3rd instar larvae of E. signifer. Conformational analysis of the EsigGOBP1-alpha-phellandrene complex, showed that MET49 and GLU38 were the key sites involved in binding. These results demonstrated that EsigGOBP1 is a key odorant-binding protein in E. signifer larvae, which recognizes and transports eight key volatiles from eucalyptus trunk, especially the main eucalyptus trunks volatile, alpha-phellandrene. Taken together, our results showed that EsigGOBP1 is involved in host selection of E. signifer larvae, which would aid in developing EsigGOBP1 as molecular targets for controlling pests at the larval stage.

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Section: Discussionmentioning

confidence: 99%

EsigGOBP1: The Key Protein Binding Alpha-Phellandrene in Endoclita signifer Larvae

Hao

Zhende

et al. 2022

IJMS

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“…The recombinant protein EcorOBP15-m1 was expressed in E. coli using standard protocols. , In order to solubilize the protein, the pellet was dissolved with 8 M urea/5 mM DTT for 1 h at room temperature and then dialyzed for 3 days against 50 mM Tris HCl pH 7.4 at 4 °C. The solubilized protein was then purified by two chromatographic steps on anion-exchange HiPrep-Q (GE-Healthcare) column.…”

Section: Methodsmentioning

confidence: 99%

Activity of Single Insect Olfactory Receptors Triggered by Airborne Compounds Recorded in Self-Assembled Tethered Lipid Bilayer Nanoarchitectures

Kleinheinz,

D’Onofrio,

Carraher

et al. 2023

ACS Appl. Mater. Interfaces

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Membrane proteins are among the most difficult to study as they are embedded in the cellular membrane, a complex and fragile environment with limited experimental accessibility. To study membrane proteins outside of these environments, model systems are required that replicate the fundamental properties of the cellular membrane without its complexity. We show here a self-assembled lipid bilayer nanoarchitecture on a solid support that is stable for several days at room temperature and allows the measurement of insect olfactory receptors at the single-channel level. Using an odorant binding protein, we capture airborne ligands and transfer them to an olfactory receptor from Drosophila melanogaster (OR22a) complex embedded in the lipid membrane, reproducing the complete olfaction process in which a ligand is captured from air and transported across an aqueous reservoir by an odorant binding protein and finally triggers a ligand-gated ion channel embedded in a lipid bilayer, providing direct evidence for ligand capture and olfactory receptor triggering facilitated by odorant binding proteins. This model system presents a significantly more user-friendly and robust platform to exploit the extraordinary sensitivity of insect olfaction for biosensing. At the same time, the platform offers a new opportunity for label-free studies of the olfactory signaling pathways of insects, which still have many unanswered questions.

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“…A large number of OBP genes have been identified in aphids, and OBP3/7 from Sitobion avenae , Rhopalosiphum padi and Acyrthosiphon pisum were discovered to detect semiochemicals 14–17 . In particular, OBP9 from M. persicae , S. avenae and A. pisum exhibited stronger binding ability toward semiochemicals than OBP3 and OBP7, 18–21 indicating that OBP9 is critical in recognizing natural semiochemicals and their analogs in insect chemical communication systems.…”

Section: Introductionmentioning

confidence: 99%

Rational design, synthesis and binding mechanisms of novel benzyl geranate derivatives as potential eco‐friendly aphid repellents

Pan,

Yang,

Liu

et al. 2023

Pest Management Science

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BACKGROUNDThe push‐pull strategy is considered as a promising eco‐friendly method for pest management. Plant volatile organic compounds (PVOCs) act as semiochemicals constitute the key factor in implementing this strategy. Benzyl alcohol and geraniol, as functional PVOCs, were reported to regulate insect behavior, showing the potential application in pest control. Using geraniol as lead, a geraniol derivative 5i with fine repellent activity was discovered in our previous work. In order to explore novel, eco‐friendly aphid control agents, a series of benzyl geranate derivatives was designed and synthesized using 5i as the lead and benzyl alcohol as the active fragment.RESULTSBenzyl alcohol was firstly evaluated to have repellent activity to Acyrthosiphon pisum. Based on this repellent fragment, a series of novel benzyl geranate derivatives was rationally designed and synthesized using a scaffold‐hopping strategy. Among them, compound T9, with a binding affinity (Kd: 0.43 μM) and a substantial repellency of 64.7% against A. pisum, is the most promising compound. Molecule docking showed that hydrophobic and hydrogen‐bonding interactions substantially influenced the binding affinity of compounds with ApisOBP9. Additionally, T9 exhibited low‐toxicity to honeybees and ladybugs.CONCLUSIONUsing a simple scaffold‐hopping strategy combined with active fragment benzyl alcohol, a new derivative T9, with high aphid‐repellent and low‐toxicity to non‐target organisms, can be considered as a novel potential eco‐friendly aphid control agent for sustainable agriculture.This article is protected by copyright. All rights reserved.

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Aphid Odorant-Binding Protein 9 Is Narrowly Tuned to Linear Alcohols and Aldehydes of Sixteen Carbon Atoms

Cited by 11 publications

References 42 publications

EsigGOBP1: The Key Protein Binding Alpha-Phellandrene in Endoclita signifer Larvae

EsigGOBP1: The Key Protein Binding Alpha-Phellandrene in Endoclita signifer Larvae

Activity of Single Insect Olfactory Receptors Triggered by Airborne Compounds Recorded in Self-Assembled Tethered Lipid Bilayer Nanoarchitectures

Rational design, synthesis and binding mechanisms of novel benzyl geranate derivatives as potential eco‐friendly aphid repellents

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