API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin

Laughon, Barbara E.; Syed, Salam A.; Loesche, Walter J.

doi:10.1128/jcm.15.1.97-102.1982

Cited by 264 publications

(135 citation statements)

References 25 publications

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“…The degradation was not affected by the protease inhibitors tested. C. sputigena is known to produce sialidase and glycosidases [3,19]. Partially and fully deglycosylated forms of lactoferrin have increased electrophoretic mobilities similar to those observed for the 77 K and 74 K protein bands respectively [20].…”

Section: Resultsmentioning

confidence: 99%

Degradation of lactoferrin by periodontitis-associated bacteria

Alugupalli

1996

FEMS Microbiology Letters

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The degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. Fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. Bacteroides forsythus and Peptostreptococcus micros. All strains of P. gingivalis tested degraded lactoferrin. The degradation was sensitive to protease inhibitors, cystatin C and albumin. The degradation by C. sputigena was not affected by the protease inhibitors and the detected lactoferrin fragments exhibited electrophoretic mobilities similar to those ascribed to deglycosylated forms of lactoferrin. Furthermore a weak or absent reactivity of these fragments with sialic acid-specific lectin suggested that they are desialylated. The present data indicate that certain bacteria colonizing the periodontal pocket can degrade lactoferrin. The presence of other human proteins as specific inhibitors and/or as substrate competitors may counteract this degradation process.

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Section: Resultsmentioning

confidence: 99%

Degradation of lactoferrin by periodontitis-associated bacteria

Alugupalli

1996

FEMS Microbiology Letters

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“…Blackpigmented species were separated into P. gingivalis, P. intermedia and P. melaninogenica by growth aerobically, production of indoie and esculin. fluorescence in long-wave ultraviolet light and analyses by Api-Zym (Laughon et al 1982, Slots & Reynolds 1982. The spirochetes were characterized as previousiy described (Fiehn 1987, Fiehn & Westergaard 1986).…”

Section: Methodsmentioning

confidence: 99%

Development of resistance to metronidazole and minocycline in vitro

Larsen

Fiehn

1997

J Clinic Periodontology

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By local delivery of antibiotics to periodontal pockets, very high initial concentrations are often quickly succeeded by subinhibitory concentrations, which may facilitate development of bacterial resistance. The purpose of the present study was to investigate possible development of resistance in suspected periodontal pathogens after exposure to subinhibitory concentrations of metronidazole and minocycline. The minimal inhibitory concentration (MIC) of 18 reference strains and 12 clinical isolates was determined by a broth dilution method. Subsequently, all strains with MIC < 8 micrograms/ml were exposed to serial passage on plates containing subinhibitory and gradually increasing concentrations of antibiotics, until growth was inhibited. Initially, most strains were inhibited at < or = 0.250 microgram/ml of minocycline and < or = 0.5 microgram/ml of metronidazole, though A. actinomycetemcomitans was resistant to metronidazole. After growth at subinhibitory concentrations, 8 strains survived 1-2 x and 11 stains survived 8-32 x their initial MIC of metronidazole, growing at up to 8 micrograms/ml. All A. actinomycetemcomitans survived 8-64 x their initial MIC of minocycline, growing at > or = 2 micrograms/ml, while all other strains were inhibited at < or = 0.250 microgram/ml, corresponding to a 1-8 x increase in their initial MIC. Thus, development of resistance was observed for periodontal bacteria growing at up to 64 x their initial MIC, but the final level of resistance was moderate.

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“…The ability of aerobic growth was tested by incubation in air with 5% CO2 on ETSA. This set of tests allowed the presumptive identification of some predominant bacteria of the subgingival plaque (Qadri & Johnson 1981, Laughon et al 1982a, b, Gusberti & Syed 1984, see Table 1. In addition, each plaque sample was plated onto CFAT (Zylber & Jordan 1982); Actinomyces naeslundii and A. viscosus were identified based on colony morphology and catalase activity.…”

Section: Methodsmentioning

confidence: 99%

Changes in subgingival microbiota during puberty A 4-year longitudinal study

et al. 1992

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Gusberti FA, Mombelli A, Lang NP and Minder CE: Changes in subgingival microbiota during puberty. A 4-year longitudinal study. J Clin Periodontol 1990; 17: 685-692. Abstract. It was the purpose of the present investigation to monitor the composition of the subgingival microbiota at selected sites in individuals passing through puberty and to correlate observed changes with the development of pubertal maturation. Between the ages of 11 and 14 years, pubertal and skeletal maturation was monitored annually in 22 boys and 20 girls. During this time, subgingival microbial samples were taken every 4th to 5th month (10 times in 4 years) mesially of the upper first molars. High values in total bacterial counts were reached after the onset of puberty, followed by a decrease towards the end of the observation period. The frequency of detection of Actinomyces odontolyticus and of Capnocytophaga sp. increased with time. The frequencies of other selected species, specifically of black pigmenting Bacteroides sp. were not found to increase when tested by hnear and quadratic models of time trend. However, a statistically significant rise in the frequency of detecting B. intermedius and B. melaninogenicus was noted in the initial pubertal phase identified by the onset of testicular growth in boys (/7 = O.O5). A significant relationship also existed between testes growth and increase of A. odontolyticus (p<0.01). In girls, a similar increase was obtained for A. odontolyticus when studied in relation to the Tanner scores for breast development {p<0.0\). The changes observed in the subgingival microbiota during puberty may be related to the development of gingivitis, which was demonstrated by a higher tendency for gingival bleeding during the course of the pubertal maturation process.

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API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin

Cited by 264 publications

References 25 publications

Degradation of lactoferrin by periodontitis-associated bacteria

Degradation of lactoferrin by periodontitis-associated bacteria

Development of resistance to metronidazole and minocycline in vitro

Changes in subgingival microbiota during puberty A 4-year longitudinal study

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