Apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing on root canal disinfection in dog teeth

Cohenca, Nestor; Heilborn, Carlos; Johnson, James D.; Flores, Daniel Silva-Herzog; Ito, Izabel Yoko; Silva, Léa Assed Bezerra da

doi:10.1016/j.tripleo.2009.08.029

Cited by 63 publications

(66 citation statements)

References 26 publications

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“…A series of reports from two controlled studies has been reported in which clinical revascularization procedures similar to those described before were performed after the induction of pulp necrosis and apical lesions in dogs. The efficacy of the triple antibiotic mix in eliminating cultivable bacteria from the root canals was reported to be 70-78% (Windley et al, 2005;Cohenca et al, 2010). While this may be an improvement over traditional disinfection, culturing of bacteria following antibiotic application may be problematic, since bacteria may be in the viable but uncultivable state, and the antibiotics are difficult to inactivate in the culture medium (Fig.…”

Section: Outcome Of Current Clinical Revascularization Techniquesmentioning

confidence: 99%

The Microbial Challenge to Pulp Regeneration

Fouad

2011

Adv Dent Res.

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Pulp regeneration is considered in cases where the dental pulp has been destroyed because of microbial irritation. Diverse oral and food-borne micro-organisms are able to invade the pulp space, form biofilm on canal walls, and infiltrate dentinal tubules. Prior to pulp regeneration procedures, the pulp space and dentinal walls need to be sufficiently disinfected to allow for and promote regeneration. The necessary level of disinfection is likely higher than that accepted for traditional endodontic therapy, because in traditional techniques the mere lowering of bacterial loads and prevention of bacterial access to periapical tissues is conducive to healing. Moreover, several of the non-specific antimicrobials used in traditional endodontic therapy may cause significant changes in remaining dentin that interfere with its inherent potential to mediate regeneration. Non-specific antimicrobials also suppress all microbial taxa, which may allow residual virulent micro-organisms to preferentially repopulate the pulp space. Therefore, it is important for endodontic pathogens to be studied by molecular methods that allow for a broad depth of coverage. It is then essential to determine the most effective protocols to disinfect the pulp space, with minimal disruption of remaining dentin. These protocols include the topical use of effective antibiotics, including newer agents that have demonstrated efficacy against endodontic pathogens.

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Section: Outcome Of Current Clinical Revascularization Techniquesmentioning

confidence: 99%

The Microbial Challenge to Pulp Regeneration

Fouad

2011

Adv Dent Res.

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“…O primeiro estudo in vivo com o uso do EndoVac foi realizado por Cohenca et al (2010). Os autores trabalharam em cães que possuíam dentes imaturos com presença de periodontite apical (n=72).…”

Section: Sistema Endovacunclassified

“…Os trabalhos in vivo são ainda escassos. Em um deles Cohenca et al (2010), mostrou que o preparo com uso do sistema EndoVac reduziu E. faecalis em dentes imaturos de cães a níveis estatisticamente iguais aos encontrados em amostras coletadas após o uso de medicação intracanal com pasta triantibiótica.…”

Section: Introductionunclassified

Avaliação in vivo da redução microbiana após preparo do canal radicular com auxílio do sistema EndoVac

Bitencourt¹

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"O conhecimento científico é cumulativo, dependente do desenvolvimento de novas tecnologias e idéias. Nossas verdades permanecem verdadeiras até que alguém demonstre que elas são incompletas. No mínimo, esta perspectiva deveria nos ensinar a defender nossas verdades temporárias com a humildade de quem aprende com o passado." The present study aimed to analyze in vivo the effectiveness of root canal preparation on bacterial reduction in patients's teeth with primary apical periodontitis. Marcelo Gleiser RESUMOSamples were collected from 20 patients before (S1) and after (S2) preparation with ProTaper rotary files, varying only the technique used for irrigation and aspiration:Group A (conventional irrigation -n = 10) and Group B (with the aid of EndoVac system -n = 10). After extraction, the DNA present in the samples was quantified by real-time PCR with the SYBR Green method, identifying the number of 16SrRNA gene copies. In all samples, except for a post-preparation case with EndoVac, copies of the target gene were identified. Average for all cases was 1,6 X 10 8 and 8,8 X 10 5 copies of the 16SrRNA, before and after preparation, respectively. For the groups separately, the same values were 2,0 X 10 8 and 5,5 X 10 5 (conventional) and 1,1 X 10 8 and 1,2 X 10 6 (EndoVac). The mean percentage of reduction was 97.52% (97.02% for the conventional and 98.04% for the EndoVac). The Mann-Whitney test concluded that both techniques significantly reduced the microrganisms after preparation (p<0,0001), with no differences between them (p=0,9705). None of the techniques were effective in the complete elimination of bacteria under this methodology. The antibacterial efficacy of the EndoVac system under this methodology was similar to that obtained with conventional irrigation and aspiration.

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“…De acordo com esta abordagem, realiza-se a desinfecção do canal radicular, empregando irrigação com hipoclorito de sódio, seguida da aplicação de uma pasta triantibiótica (Trimix), composta de ciprofloxacino, metronidazol e minociclina Sato et al, 1996;Windley et al, 2005;Murray et al, 2007;Reynolds et al, 2009;Cohenca et al, 2010;Silva et al, 2010;Wang et al, 2010;Barnes e Patel, 2011;Lin e Rosenberg, 2011;Lovelace et al, 2011;Nosrat et al, 2011). Esta pasta permanece no canal radicular por períodos que variam de dias a meses de acordo com os diferentes trabalhos (Banchs e Trope, 2004;Windley et al, 2005;Thibodeau e Trope, 2007;Ding et al, 2009;Akgun et al, 2009;Kim et al, 2010;Petrino et al, 2010;Lovelace et al, 2011).…”

Section: Introductionunclassified

“…Após a remoção da pasta, efetua-se ou não a indução de sangramento para preencher o canal radicular, promovendo a formação de coágulo sanguíneo abaixo da junção amelocementária. Uma camada de MTA é inserida sobre o coágulo sanguíneo e efetua-se o selamento coronário (Banchs e Trope, 2004;Hargreaves et al, 2008;Jung et al, 2008;Reynolds et al, 2009;Cohenca et al, 2010;Silva et al, 2010;Yamauchi et al, 2011). O objetivo desta técnica é permitir a revascularização do canal radicular e a continuidade da formação radicular, por meio do desenvolvimento normal da espessura das paredes dentinárias, do comprimento da raiz e da morfologia apical Huang, 2008;Jung et al, 2008;Trope, 2008;Lovelace et al, 2011;Nosrat et al, 2011).…”

Section: Introductionunclassified

Resposta celular e molecular do tecido conjuntivo de camundongos e medicações intracanal

Pereira¹

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Apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing on root canal disinfection in dog teeth

Cited by 63 publications

References 26 publications

The Microbial Challenge to Pulp Regeneration

The Microbial Challenge to Pulp Regeneration

Avaliação in vivo da redução microbiana após preparo do canal radicular com auxílio do sistema EndoVac

Resposta celular e molecular do tecido conjuntivo de camundongos e medicações intracanal

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