Apical transport of Crumbs maintains epithelial cell polarity

Fletcher, Georgina; Thompson, B. J.

doi:10.1101/592311

Cited by 2 publications

(2 citation statements)

References 148 publications

(206 reference statements)

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“…We co-overexpressed Crb and spastin in the SG to test whether disruption of MTs affects apical transport of Crb. A similar approach was taken in a recent study, where overexpression of Crb provided a highly sensitive genetic background for identifying components involved in Crb trafficking (Aguilar-Aragon et al, 2019). Consistent with previous findings that Crb expression expands apical membranes in epithelial cells Chung et al, 2017;Myat and Andrew, 2002;Wodarz et al, 1993), overexpression of Crb resulted in enlarged apical areas of SG cells during invagination ( Figure S3B-S3B').…”

Section: Enrichment Of Key Apical and Junctional Proteins Is Mt-depensupporting

confidence: 85%

“…One such protein is an apical transmembrane protein Crb, and Rab11 helps maintain apical Crb in the Drosophila ectoderm (Roeth et al, 2009). In the Drosophila SG and follicle cells, Crb is apically transported along MTs by the dynein motor (Myat and Andrew, 2002) (Aguilar-Aragon et al, 2019). Importantly, in Drosophila tracheae, loss of crb impairs apical constriction during the internalization process (Letizia et al, 2011).…”

Section: Enrichment Of Key Apical and Junctional Proteins Is Mt-depenmentioning

confidence: 99%

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Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination

Chung

2019

Preprint

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The formation of an epithelial tube is a fundamental process for organogenesis. Mechanisms underlying coordination between actomyosin and microtubules (MTs) during tube formation is beginning to be elucidated. During the Drosophila embryonic salivary gland (SG) invagination, Folded gastrulation (Fog)-dependent Rho-associated kinase (Rok) promotes contractile apical myosin formation to drive apical constriction. MTs are also crucial for the effective change in apical area and are required for forming and maintaining apicomedial myosin. Here, we show that MT-dependent intracellular trafficking has a role in regulating apical constriction during SG invagination. Key components involved in protein trafficking, including dynein heavy chain, Rab11 and Nuclear fallout (Nuf), are apically enriched near the invagination pit in a MT-dependent manner during SG invagination. This enrichment is crucial for apical constriction as disruption of the MT networks or intracellular trafficking impairs formation of apicomedial myosin, which leads to apical constriction defects. We show that apical transport of several proteins along MTs, either in a Rab11-dependent or independent manner, mediates clustered apical constriction during SG invagination. Key proteins that are transported include the Fog ligand, the apical determinant protein Crb, a key adherens junction protein E-Cad and the scaffolding protein Bazooka/Par3, and knockdown of these genes in the SG results in apical constriction defects. These results define a role of MT-dependent intracellular trafficking in regulating the actomyosin networks as well as cell junctions to coordinated cell behaviors during tubular organ formation.

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Section: Enrichment Of Key Apical and Junctional Proteins Is Mt-depensupporting

confidence: 85%

Section: Enrichment Of Key Apical and Junctional Proteins Is Mt-depenmentioning

confidence: 99%

Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination

Chung

2019

Preprint

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Cytocortex-dependent dynamics of Drosophila Crumbs controls junctional stability and tension during germ band retraction

Bajur

Iyer

Knust

2019

Journal of Cell Science

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During morphogenesis, epithelia undergo dynamic rearrangements, which requires continuous remodelling of junctions and cell shape, but at the same time mechanisms preserving cell polarity and tissue integrity. Apico-basal polarity is key for the localisation of the machinery that enables cell shape changes. The evolutionarily conserved Drosophila Crumbs protein is critical for maintaining apico-basal polarity and epithelial integrity. How Crumbs is maintained in a dynamically developing embryo remains largely unknown. Here, we applied quantitative fluorescence techniques to show that, during germ band retraction, Crumbs dynamics correlates with the morphogenetic activity of the epithelium. Genetic and pharmacological perturbations revealed that the mobile pool of Crumbs is fine-tuned by the actomyosin cortex in a stage-dependent manner. Stabilisation of Crumbs at the plasma membrane depends on a proper link to the actomyosin cortex via an intact FERM-domainbinding site in its intracellular domain, loss of which leads to increased junctional tension and higher DE-cadherin (also known as Shotgun) turnover, resulting in impaired junctional rearrangements. These data define Crumbs as a mediator between polarity and junctional regulation to orchestrate epithelial remodelling in response to changes in actomyosin activity. This article has an associated First Person interview with the first author of the paper.

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Apical transport of Crumbs maintains epithelial cell polarity

Cited by 2 publications

References 148 publications

Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination

Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination

Cytocortex-dependent dynamics of Drosophila Crumbs controls junctional stability and tension during germ band retraction

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