Apigenin effect against Leishmania tropica amastigotes in vitro

Naddaf, Nagham; Haddad, Shaden

doi:10.1007/s12639-020-01230-8

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…Moreover, it has been revealed that apigenin was effective against Leishmania tropica amastigote [66]. In addition, previous reports have shown that coumarins exhibit activity against the promastigote form of Leishmania major and Trypanosma cruzi (53,87).…”

Section: Discussionmentioning

confidence: 98%

“…Flavonoids have been shown to possess activity against Cryptosporidium parvum , Leishmania donovani , Entamoeba histolytica, and Plasmodium falciparum [ 48 , 49 , 57 , 61 ]. Moreover, it has been revealed that apigenin was effective against Leishmania tropica amastigote [ 66 ]. In addition, previous reports have shown that coumarins exhibit activity against the promastigote form of Leishmania major and Trypanosma cruzi [ 53 , 87 ].…”

Section: Discussionmentioning

confidence: 99%

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Effect of some plant extracts from Egyptian herbal plants against Toxoplasma gondii tachyzoites in vitro

2021

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Nowadays, herbal extracts are considered to be a potential source for developing new drugs that will overcome resistance to conventional chemotherapeutic agents. This study was aimed to explore the efficacy of several Egyptian plant extracts against Toxoplasma gondii infection in vitro for future development of a new, safe, and effective compound for T. gondii. Methanol extracts from Matricaria chamomilla (German chamomile), Laurus nobilis, Citrullus colocynthis, Cinnamum camphora, Boswellia scara, and Melissa officionalis plants and oil extracts (either essential or fixed oils) of some plants such as: lemon grass (Cymbopogon citratus), marjoram (Origanum majorana), watercress (Nasturtium officionale), wheat germ (Triticum aestivum), sesame (Sesamum indicum), rosemary (Salvia rosmarinus), citronella (Cymbopogon nardus), clove (Syzygum aromaticum), jojoba (Simmondsia chinesis), and basil (Ocimum basilicum) were investigated for their anti-Toxoplasma activities. The methanol extracts from C. colocynthis and L. nobilis and the oil extracts from lemon grass and marjoram were active against T. gondii with half maximal inhibitory concentrations (IC 50) of 22.86 µg/ml, 31.35 µg/ml, 4.6 µg/ml, and 26.24 µg/ml, respectively. Their selectivity index (SI) values were < 10. Interestingly, the methanol extract from M. chamomilla and oil from citronella had the lowest IC 50 values for T. gondii (3.56 µg/ml and 2.54 µg/ml, respectively) and the highest SI values (130.33 and 15.02, respectively). In conclusion, methanol extract from M. chamomilla and oil from citronella might be potential sources of novel therapies for treating toxoplasmosis.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Effect of some plant extracts from Egyptian herbal plants against Toxoplasma gondii tachyzoites in vitro

2021

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“…The macrophages were counted using Neubauer counting chamber to determine the number of live cells in 1 ml of the previous suspension. And the number of macrophages were calculated according to the following equation: The number of macrophages in 1 ml = average number of macrophages in 4 white squares×10×1000 (16) .…”

Section: Obtaining Mouse's Macrophagesmentioning

confidence: 99%

“…The effect was studied by adding mouse ' s macrophages to culture wells. Then, promastigote forms taken from a farm in the process of cultivation were added at a rate of 5 parasites per macrophage, they were previously incubated at 37C o for 24 hours (16) .…”

Section: Testing the Efficacy Of Terpinen-4-ol On Amastigote Form Of ...mentioning

confidence: 99%

“…Then, Terpinen-4-ol was added at final concentrations of 0.01, 0.02, 0.5 and 0.1%, while the same volume of distilled water was added to the control wells and the culture wells were incubated at 37Co for an additional 24 hours. Parasites were counted into 100 macrophages in each well of wells treated with Terpinen-4-ol and in control wells (16) .…”

Section: Testing the Efficacy Of Terpinen-4-ol On Amastigote Form Of ...mentioning

confidence: 99%

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A Study of the inhibitory effect of Terpinen-4-ol on Amastigote Forms of Leishmania tropica within Macrophages of Mouse in vitro

Hami

2022

IJPS

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It was recorded that Terpinen-4-ol has an anti-parasitic properties, so it will be noteworthy to intensify the studies about this compound. This study aims to test the effectiveness of terpinen-4-ol on amastigote forms of Leishmania parasite in macrophages. This effect was studied by adding increasing concentrations of Terpinen-4-ol to culture wells containing mouse macrophages that were previously incubated with the promastigote forms of the parasites for 24 hours .Then, they were incubated for another 24 hours with increasing concentrations of Terpinen-4-ol. After, Parasites were enumerated into macrophages in wells either treated with Terpinen-4-ol or in control wells. Treatment with Terpinen-4-ol at concentrations (0.01%, 0.02%, 0.05%, 0.1%) (v/v) decreased the viability of the amastigote forms inside macrophages (24.02%, 32.74%, 66.72%, 100%) respectively, compared to control wells (water only). This study demonstrated an activity of Terpinen-4-ol against amastigote forms of Leishmania tropica in vitro with minimal inhibitory concentration (MIC50) value was 0.0416% (v/v).

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Chromatographic separation and estimation of natural antimalarial flavonoids in biological matrices

Chaturvedi

Malik²,

Sultana

et al. 2021

Proc.Indian Natl. Sci. Acad.

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Apigenin effect against Leishmania tropica amastigotes in vitro

Cited by 10 publications

References 22 publications

Effect of some plant extracts from Egyptian herbal plants against <i>Toxoplasma gondii</i> tachyzoites <i>in vitro</i>

Effect of some plant extracts from Egyptian herbal plants against <i>Toxoplasma gondii</i> tachyzoites <i>in vitro</i>

A Study of the inhibitory effect of Terpinen-4-ol on Amastigote Forms of Leishmania tropica within Macrophages of Mouse in vitro

Chromatographic separation and estimation of natural antimalarial flavonoids in biological matrices

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