Apigenin potentiates the antitumor activity of 5-FU on solid Ehrlich carcinoma: Crosstalk between apoptotic and JNK-mediated autophagic cell death platforms

Gaballah, Hanaa Hibishy; Gaber, Rasha A.; Mohamed, Dareen Abd El-Aziz

doi:10.1016/j.taap.2016.12.012

Cited by 31 publications

(22 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pharmacological activities of these three flavones have been reported. Apigenin possesses anti‐inflammatory, anti‐oxidant and anticancer activities (Gaballah, Gaber, & Mohamed, ; Patil et al, ; Xu et al, ). Genkwanin shows significant anti‐tumor activity by enhancing host immunity and decreasing inflammatory cytokine levels (Wang et al, ); this compound also exhibits antioxidant properties and immunomodulatory effects on splenocytes, NK and CTL cells (Nasr‐Bouzaiene et al, ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

View full text Add to dashboard Cite

A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for investigating the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin after oral administration of Daphne genkwa extract. Plasma samples were treated by protein precipitation with acetonitrile. Analytes were detected by triple-quadrupole MS/MS with an ESI source in negative selection reaction monitoring mode. The transitions of m/z 161 → 133 for umbelliferone, m/z 269 → 117 for apigenin, m/z 283 → 268 for genkwanin and m/z 299 → 284 for hydroxygenkwanin were confirmed for quantification. Chromatographic separation was conducted using an Eclipse XDB-C column, and the applied isocratic elution program allowed for simultaneous determination of the four analytes for a total run time of 2.5 min. The linearity was validated over the plasma concentration ranges of 1.421-1421 ng/mL for umbelliferone, 0.845-845 ng/mL for apigenin, 1.025-1025 ng/mL for genkwanin and 0.845-845 ng/mL for hydroxygenkwanin. The extraction recovery rate was >82.7% for each analyte. No apparent matrix effect was observed during the bioanalysis. After full validation, the proposed method was successfully applied to compare the pharmacokinetics of these analytes between normal and arthritic rats.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Total RNA concentration and purity were determined by measuring OD 260 and OD 260/280 ratio, respectively, on a NanoDrop spectrophotometer (NanoDrop Technologies, Inc, Wilmington, DE), RNA was then stored at −80°C. Complementary DNA (cDNA) synthesis: Total RNA samples were reverse‐transcribed using the RevertAid H Minus Reverse Transcriptase (#EP0451; Thermo Fisher Scientific) to produce cDNA as previously described . The cDNA was then stored at −20°C until used for polymerase chain reaction (PCR). …”

Section: Methodsmentioning

confidence: 99%

“…Complementary DNA (cDNA) synthesis: Total RNA samples were reverse‐transcribed using the RevertAid H Minus Reverse Transcriptase (#EP0451; Thermo Fisher Scientific) to produce cDNA as previously described . The cDNA was then stored at −20°C until used for polymerase chain reaction (PCR).…”

Section: Methodsmentioning

confidence: 99%

Hesperidin promotes lysosomal biogenesis in chronically ethanol‐induced cardiotoxicity in rats: A proposed mechanisms of protection

Gaballah

Ghanem

Tahoon

et al. 2018

J Biochem & Molecular Tox

Self Cite

View full text Add to dashboard Cite

Alcohol consumption is a major global risk factor for mortality and morbidity. We aimed to delineate the mechanisms underlying the potential ameliorative effects of hesperidin against chronically ethanol‐induced cardiotoxicity. Sixty male albino rats were divided into normal control group, hesperidin‐treated control group, untreated alcoholic group, and hesperidin‐treated alcoholic group. Transcription factor‐EB (TFEB) expression levels were estimated using real‐time reverse transcription‐polymerase chain reaction. Peroxisome proliferator–activated receptor γ coactivator 1‐α (PGC1‐α), macrophage inflammatory protein‐1 α, poly‐(ADP‐ribose)‐polymerase‐1 (PARP‐1) activity, and tenascin C levels in cardiac tissues were estimated by enzyme‐linked immunosorbent assay; while tissue malondialdehyde and total antioxidant capacity were evaluated spectrophotometrically. Our data portrayed promoting lysosomal biogenesis, as judged by upregulation of TFEB expression and its target PGC1‐α, as well as decreased PARP‐1 activity and offsetting inflammation, oxidative stress, and tissue injury as the principal culprits mediating the cardioprotective effect of hesperidin in alcohol‐induced cardiotoxicity. In conclusion, hesperidin can be used as a cardioprotective agent in chronically ethanol‐induced cardiotoxicity.

show abstract

“…In case of solid tumor, tumor volumes were recorded using a vernier calipes and calculated as V (mm 3 ) = (a 2 ×b)/2 where a (smaller diameter) and b (large diameter) are perpendicular [21].…”

Section: Tumor Volumementioning

confidence: 99%

“…After washing sections with PBS, they were incubated with diaminobenzidine substrate and counterstained with Mayer's hematoxylin. Then, they were visualized in a digital camera installed on a light microscope [2,21].…”

Section: Immunohistochemical Analysis Of Bcl-2 Ki-67 Cyclin-d1mentioning

confidence: 99%

In Vivo Antitumor Activity of Phytochemical Pitc-2 Obtained From Tissue Cultured Plant Pluchea Indica on Sarcoma-180 Solid Tumor Mice Model

Goswami

Debnath

Karan

et al. 2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: PITC-2 was isolated from the methanolic root extract of tissue cultured medicinal plant Pluchea indica (L.) Less. PITC-2 is a thiophene derivative which is 2-(Prop-1-ynyl)-5(5,6-dihydroxyhexa-1,3-diynyl)-thiophene. The main objective of the study is to evaluate the in vivo antitumor activity of PITC 2 against sarcoma-180 cancer cell in Swiss albino mice.Methods: The antitumor activity was evaluated by treatment with PITC-2 at a dose of 2.5 and 5 mg/kg b.w for 21 days on sarcoma-180 mice model. Cell viability was studied using 3-(4, 5- dimethylthiazol -2-yl)-2, 5-diphenyl tetrazolium bromide assay and cell apoptosis, G1 cell cycle arrest and reduction in tumor cell proliferation were evaluated by histopathological analysis and Bcl-2, cyclic-D1, and Ki-67 protein expression through immunohistochemistry study.Results: Precisely, PITC-2 had a cytotoxic effect on various in vitro cancer cells. Significant decreases in solid tumor volume and weight along with increase lifespan also observed. The histopathological and immunohistopathological examination indicates that PITC-2 induces apoptosis, typical morphological changes and suppresses tumor cell proliferation along with G1 cell cycle arrest through the downregulation of the intratumoral expression of Bcl-2, cyclic D1, and Ki-67 and thus highlighting antiproliferative and apoptotic properties against sarcoma-180 in vivo solid tumor model.Conclusion: The present results clearly demonstrate that PITC-2 significantly inhibits sarcoma-180 cell growth in a dose-dependent manner in in vivo mice model. Besides this, the study reveals a comprehensive perception of the possible mechanism behind the antitumor activity of PITC-2 by significant changes in the morphological, hematological, biochemical parameters in sarcoma-180 cells.

show abstract

Apigenin potentiates the antitumor activity of 5-FU on solid Ehrlich carcinoma: Crosstalk between apoptotic and JNK-mediated autophagic cell death platforms

Cited by 31 publications

References 46 publications

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Hesperidin promotes lysosomal biogenesis in chronically ethanol‐induced cardiotoxicity in rats: A proposed mechanisms of protection

In Vivo Antitumor Activity of Phytochemical Pitc-2 Obtained From Tissue Cultured Plant Pluchea Indica on Sarcoma-180 Solid Tumor Mice Model

Contact Info

Product

Resources

About