Aplicação de técnicas cromatográficas e espectrométricas como ferramentas de auxílio na identificação de componentes de óleos voláteis

Brochini, Cláudia Barbosa; Lago, João Henrique G.

doi:10.1590/s0102-695x2007000200022

Cited by 12 publications

(11 citation statements)

References 13 publications

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“…Essential oils with the highest and the lowest toxicity, according to LC 50 , were selected and analyzed by gas chromatography coupled to mass spectrometry (GC-MS) on the Agilent 5975C Series GC/MS (Agilent Technologies Inc., Palo Alto, CA, USA), which incorporates a quadrupole system and is equipped with a nonpolar DB-5 column with 60 m x 0.25-mm inner diameter and 0.25-μm film thickness (Agilent J&W GC Columns, Agilent Technologies Inc., Palo Alto, CA, USA) (Brochini & Lago, 2007). A 1-mL solution of known concentration, containing its corresponding essential oil diluted in hexane, was injected at a split ratio of 1:20.…”

Section: Methodsmentioning

confidence: 99%

Fumigation and repellency of essential oils against Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) in cowpea

Oliveira

França

Barbosa

et al. 2017

Pesq. agropec. bras.

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-The objective of this work was to assess the fumigant and repellent effects of essential oils on adults of Callosobruchus maculatus and to identify the chemical composition of two of the tested essential oils. For the fumigation test, the oils of Schinus terebinthifolius, Piper aduncum, Syzygium aromaticum, Piper hispidinervum, Cymbopogon citratus, Cinnamomum zeylanicum, and the eugenol compound were tested at different concentrations on C. maculatus adults. For the repellency test, the oils of S. terebinthifolius, P. aduncum, P. hispidinervum, S. aromaticum, Jatropha curcas, and Ricinus communis were evaluated. In the fumigation test, it was observed that P. aduncum and eugenol showed the highest and lowest LC 50s , of 169.50 and 0.28 µL L -1 air, respectively. In the repellency test, the oils of S. aromaticum and P. hispidinervum were repellent to C. maculatus. Gas chromatography coupled to mass spectrometry (GC-MS) analysis of these two oils identified 42 compounds, of which safrole was the main component of P. hispidinervum and eugenol of S. aromaticum. The essential oils of S. aromaticum, C. zeylanicum, and the eugenol compound are the most promising to control C. maculatus, via fumigation.

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Section: Methodsmentioning

confidence: 99%

Fumigation and repellency of essential oils against Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) in cowpea

Oliveira

França

Barbosa

et al. 2017

Pesq. agropec. bras.

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“…Part of the crude oil from collection of January 2004 (approximately 150 mg) was submitted to column chromatography over silica gel soaked with AgNO 3 (15%) (50 g, 55 x 2 cm) eluted with pentane (100 mL), CH2Cl2 (150 mL) and mixtures of CH 2 Cl 2 :Me 2 CO 9:1 (100 mL) and 8:2 (50 mL), to afford fifty four fractions, which were analyzed by GC/FID and pooled together in eleven groups (A-1 to A-11) (Brochini et al, 1999;Brochini & Lago, 2007). This analysis indicated that some groups were composed by one major derivative and had their NMR spectra recorded.…”

Section: Volatile Oil Fractionationmentioning

confidence: 99%

Chemical composition and seasonal variation of the volatile oils from leaves of Michelia champaca L., Magnoliaceae

Lago¹,

Fávero²,

Romoff³

2009

Rev. bras. farmacogn.

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RESUMO: "Composição química e variação sazonal dos óleos voláteis das folhas de Michelia champaca L., Magnoliaceae". Os óleos voláteis das folhas de Michelia champaca L., coletadas bimestralmente ao longo de um ano (quatro vezes no décimo quinto dia de janeiro, março, maio, julho, setembro e novembro de 2004), foram submetidos à análise por CG/DIC e CG-EM, de onde foram identificados treze componentes. Adicionalmente, parte do óleo obtido na coleta de janeiro foi submetida a fracionamento em gel de sílica impregnada com AgNO 3 fornecendo cinco dos principais sesquiterpenos (β-elemeno, β-cariofileno, α-humuleno, β-selineno e α-cadinol). Os dados obtidos mostram uma variação significativa na proporção dos componentes, a qual pode estar associada a parâmetros microclimáticos em cada período de coleta. Unitermos: Michelia champaca, terpenoides, variação sazonal.ABSTRACT: The volatile oils from leaves of Michelia champaca L. collected bimonthly during one year (four times on the fifteenth day of January, March, May, July, September, and November -2004) were subjected to GC/FID and GC-MS analysis, from which thirteen components were identified. Additionally, part of the oil obtained from January collection was subjected to fractionation over silica gel soaked with AgNO 3 to afford five of the main sesquiterpenes (β-elemene, β-caryophyllene, α-humulene, β-selinene, and α-cadinol). The obtained data showed a significative variation in the proportions of the components, which could be associated to climatic parameters in each collection periods.

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“…A ocorrência dos dois isômeros pode ser comprovada através da análise do espectro de RMN de 13 C do óleo essencial bruto (Brochini et al, 1999;Brochini & Lago, 2007), o qual mostrou, dentre outros, sinais relativos a dois carbonos carbonílicos em δ 190,9 e em δ 191,4, os quais puderam ser atribuídos aos carbonos C-1 do neral e do geranial, respectivamente. Além destes, outros sinais foram detectados entre δ 164,0-122,4, relativos aos carbonos sp 2 C-2/C-3 e C-6/C-7 da mistura isomérica.…”

Section: Resultsunclassified

“…Os espectros de RMN foram registrados em um espectrômetro Bruker, modelo DPX-300, operando a 75 MHz para o núcleo do carbono-13, sendo utilizado clorofórmio-d (Aldrich) como solvente e o pico residual do derivado não deuterado empregado como padrão interno (Brochini & Lago, 2007).…”

Section: Análise Por Ressonância Magnética Nuclear (Rmn)unclassified

Variação dos teores de constituintes voláteis de Cymbopogon citratus (DC) Staf, Poaceae, coletados em diferentes regiões do Estado de São Paulo

Furlan¹,

Martins²,

Rodrigues³

et al. 2010

Rev. bras. farmacogn.

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RESUMO:O óleo essencial extraído de Cymbopogon citratus (DC) Staf, Poaceae, é rico em citral sendo amplamente empregado na indústria química e farmacêutica. Os objetivos desse estudo foram avaliar o conteúdo de citral entre populações de C. citratus coletados em duas regiões diferentes (Ibiúna e Pindamonhangaba, Estado de São Paulo). O óleo essencial extraído de uma população cultivada em Pindamonhangaba-SP apresentou uma concentração alta de citral (aproximadamente 96,0%), enquanto que o óleo extraído da população de Ibiúna-SP apresentou, além de citral, proporções elevadas de outro monoterpeno, o geraniol. Adicionalmente, os óleos essenciais obtidos desta população de C. citratus foram extraídos e analisados ao longo de 24 h (a cada 3 h), sendo que o teor de citral foi maior no período da noite, o que mostra a importância de estudos que avaliam as diferenças químicas associadas às mudanças circadianas. Os componentes dos óleos essenciais foram identificados por RMN de 13 C, CG/EM e CG através do índice de Kovats. Unitermos:Cymbopogon citratus, constituintes voláteis, citral.ABSTRACT: "Variation in the amounts of volatile constituents of Cymbopogon citratus (DC) Staf, Poaceae, collected in different regions of São Paulo State." The essential oil extracted from Cymbopogon citratus (DC) Staf, Poaceae, is rich in citral and it has many applications in chemical and pharmaceutical industry. The aims of this study were to evaluate the amount of citral in populations of C. citratus collected in two different regions (Ibiúna and Pindamonhangaba, São Paulo State). The essential oil extracted of a cultivated population in Pidamonhangaba-SP, showed a high content of citral (approximately 96.0%), while the essential oil from Ibiuna-SP, showed citral and high amounts of another monoterpene, geraniol. Additionally, the essential oils from this population of C. citratus were extracted and analyzed during 24 h (each 3 h), being the major concentration of citral in the night period, indicating that studies which evaluated the chemical differences associated to circadian changes. The components of essential oils were identified through 13 C NMR, GC/MS, GC and Kovat's index.

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Aplicação de técnicas cromatográficas e espectrométricas como ferramentas de auxílio na identificação de componentes de óleos voláteis

Cited by 12 publications

References 13 publications

Fumigation and repellency of essential oils against Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) in cowpea

Fumigation and repellency of essential oils against Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) in cowpea

Chemical composition and seasonal variation of the volatile oils from leaves of Michelia champaca L., Magnoliaceae

Variação dos teores de constituintes voláteis de Cymbopogon citratus (DC) Staf, Poaceae, coletados em diferentes regiões do Estado de São Paulo

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