The need to manage water resources and the way they behave in the face of changes in land use are essential for managing a watershed. In this context, a large basin modeling tool was applied to simulate the flow regime of the Ibicuí River basin considering two land use scenarios. The results were used to analyze the changes in the maximum and minimum annual and monthly average flows. The greatest change in land use was the forest coverage reduction associated to agriculture land use. As a result, an increase in the average monthly flows and in the reference flow Q90 was verified. In some sub-basins, a significant modification in the reference Q90 flow and maximum flow was verified. The Mann Whitney test showed that the results were accurate. The results indicated that changes in forest coverage and land use have an impact in the flow regime in the Ibicuí Watershed. The Impacts of Changes in Land Use and Coverage on the Ibicuí River Basin procedure used in this research can be applied to simulate future scenarios of land use and its impacts in the sub-basin´s flow rates.