Aplicación Del Cultivo De Tejidos en La Multiplicación Y Conservación De Los Recursos Fitogenéticos

Ganchozo, Blanca Soledad Indacochea; Villacreses, Johann Carlos Parrales; Ganchozo, Bertha Azucena Zhindón; Lucio, Yhony Alfredo Valverde; Indacochea, Blanca Viviana Álvarez; Indacochea, Paúl Alberto Choez

doi:10.26820/978-9942-787-98-9

Cited by 3 publications

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“…La producción de cultivos de importancia económica, para el mercado local, nacional y externo, requiere contar con genotipos adaptados a cada lugar o región para que puedan soportar las condiciones a las que están sometidos durante los diferentes estados de desarrollo fenológico; esto hace que las investigaciones estén orientadas a lograr suplir estos niveles de exigencias, para una producción exitosa. La biotecnología, a través del cultivo in vitro de tejidos, tiene la ventaja de "multiplicar rápidamente una variedad de la cual sólo existen pocos individuos" (Aguirre et al, 2016); ésta, constituye una herramienta para obtener plantas uniformes a partir de un tejido, explante (Mroginski & Roca, 1993); y una alternativa para una propagación rápida, segura y obtener plantas sanas, libres de patógenos. La propagación vegetativa de la fresa Fragaria x ananassa Duch.…”

Section: Introductionunclassified

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Gonzales-Arteaga,

Rodríguez-Layza,

Romero-Rivas

et al. 2023

Agroind. sci.

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The objective was to determine the response of three strawberrry varieties (Fragaria x ananassa Duch.) to the aplication of two plant growth regulators: indol acetic acid (IAA) and 6-Benzyl aminopurine (BAP) added to Murashige & Skoog médium in strawberry micropropagation. The complete randomized block design was used and three strawberry varieties ‘Aromas’, ‘Albion’ and ‘Camarosa’ were considered as trials. MS medium for shoot regeneration in initial stage was supplemented with BAP 0.5 mgL-1 and IAA 0.6 mgL-1. Shoot proliferation MS medium was supplemented with BAP 0.5 mgL-1 in multiplication stage and MS medium hormone free was used for rooting induction. It was determined that after 32 days of cultivation the combination of IAA and BAP significantly affected shoot regeneration of apical shoot tips of three strawberry varieties. The greatest effect was caused on ‘Camarosa’. In addition, results on shoot multiplication using BAP indicated that ‘Albion’ performed better in shoot proliferation in terms of shoots per explants reaching 2.51 compared to ‘Camarosa’ and ‘Aromas’ with significant differences. However rooting medium with no growth regulators improves root development with best response of ‘Camarosa’ root length reaching 4.76 cm.

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Section: Introductionunclassified

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Gonzales-Arteaga,

Rodríguez-Layza,

Romero-Rivas

et al. 2023

Agroind. sci.

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“…Selva (Mena et al, 2017); y en la variedad Camino Real el contenido de antocianinas es de 5,9 mg l -1 (Cárdenas-Navarro et al, 2019), en Camarosa entre 45,64 a 92,83 y en Osogrande de 30,43 a 48,95 en mg equivalentes de Cianidin-3glucósido 100 g -1 (Carvajal de Pabón et al, 2012). Para la desinfección de explantes, la combinación de etanol al 70% e hipoclorito de sodio, permite niveles de contaminación inferiores; y al incrementar la concentración de hipoclorito de sodio y el tiempo de exposición, el número de explantes contaminados disminuye; pero, incrementa la cantidad de explantes no viables (Bedoya-Perez et al, 2016); que produce grandes pérdidas en el cultivo in vitro, hasta un 90% (Aguirre et al, 2016). En la micropropagación, la elección de las plantas madres donadoras de explantes es muy importante, éstas no deben mostrar síntomas de enfermedades y presentar características fenotípicas de las variedades correspondientes (Mamani-Sánchez & Murillo-García, 2020), se recomienda que provengan de un ambiente controlado para disminuir agentes contaminantes en el establecimiento in vitro, no obstante también depende del tipo de explante.…”

Section: Introductionunclassified

Establecimiento in vitro de Fragariax ananassa var. Aroma a partir de yemas de corona

Arteaga

Layza

Romero-Rivas

et al. 2022

Manglar

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The objective of the research was to determine a protocol for disinfection and in vitro establishment of explants from strawberry crown buds Fragaria x ananassa Var. Aroma, from the Minor Town Center Mallampampa, Huancabamba and the La Florida sector, Chontabamba district, Oxapampa, Pasco, Peru, through a randomized complete block design. Three disinfection methods were used T1 (adapted from Coba, 2009), T2 (adapted from Sánchez-Cuevas and Salaverría, 2004)and T3 (adapted from Mamani, 2005) and in the establishment were the treatments, t1 (MS+ BAP 0.5 mg l -1+ NAA 0.5 mg l-1), t2 (MS+BAP 0.5 mg l-1+ NAA 0.3 mg l-1+ GA3 0.2 mg l-1), t3 (MS+BAP 0.5 mg l-1+ IAA 0.6 mg l-1), t4 (MS+BAP 0.5 mg l-1+ IAA 0.5 mg l-1+ GA3 0.3 mg l-1) and t5 (MS without regulators). At 36 days after inoculation (dai),the best disinfection treatment was T3, because it presented less contamination by bacteria and less phenolization and without significant differences in fungi and explant survival. The best establishment medium was t3, for present a lower degree of phenolization, greater survival, sprouting and number of shoots and leaves.

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Development of an ecological strategy for the control of downy mildew (Pseudoperonospora cubensis) in cucumber cultivation (Cucumis sativus L.)

Gabriel-Ortega

Pereira-Murillo

Ayón-Villao

et al. 2015

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Downy mildew is a severe disease of cucumber worldwide. The oomycete Pseudoperonospora cubensis cause it and once it is established in a region, the infection spreads rapidly, causing significant loss of yield and fruit quality. The objective of the research was to develop an ecological strategy for the control of downy mildew in cucumber. The treatments were arranged in a completely randomized experimental design with an alternation of chemical and biological fungicides. The treatments were: T1: systemic fungicide (Ridomil Gold, 2.5 g/l) alternating with a contact fungicide (Bravo 2.5 ml/l), T2: CustomBio 5 (Bacillus-based fungicide, 3ml/l), T3: control (water), T4: Trichoderma sp. (3 ml/l), T5: systemic fungicide (Ridomil Gold 2.5g/l) alternating with CustomBio 5 (3 ml/l), and T6: systemic fungicide (Ridomil Gold 2.5g/l) alternating with Trichoderma sp. (3 ml/l). The following variables were evaluated: stem thickness, plant height, number and weight of fruits, yield, the area under the relative progress curve (AUDPCr), and economic analysis of each treatment. The results showed that the best treatments were T1 and T6, with an AUDPCr of 11.89% and 12.10%, respectively. Treatments T6 and T1 showed the best yield, as well. The profitability analysis showed that all the alternatives were profitable with a Benefit/Cost>1 ratio. However, the treatments T6 and T1 were the most useful. We recommend this control strategy to reduce the use of chemical fungicides and, at the same time, obtain an efficient control of the disease, which guarantees a significant yield of high-quality fruit.

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Aplicación Del Cultivo De Tejidos en La Multiplicación Y Conservación De Los Recursos Fitogenéticos

Cited by 3 publications

References 0 publications

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Establecimiento in vitro de Fragariax ananassa var. Aroma a partir de yemas de corona

Development of an ecological strategy for the control of downy mildew (Pseudoperonospora cubensis) in cucumber cultivation (Cucumis sativus L.)

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