The current study aimed to evaluate nutrition of black soldier fly larvae (BSFL) using Van Soest and fatty acid profile reared at different media. A total of five different treatments were used in this study as follows: fruit waste, food waste, tofu by-product, 50% fruit waste + 50% food waste, and 50% fruit waste + 50% tofu by-product. The parameters observed were fatty acid profile (saturated and non-saturated fatty acid) and fiber composition (Neutral Detergent Fiber, Acid Detergent Fiber, Cellulose, hemicellulose, and lignin). Fatty acid profile analysis was done using gas chromatography and fiber composition using Van Soest method. The result presented the composition of fatty acid in BSFL dominated by saturated fatty acid, namely lauric acid (34.28 – 38.39%), palmitic acid (17.45 –19.25%), arachidic acid (8.8 – 10.41%), myristic acid (7.35 – 9.36%), heptadecanoic acid (2.03– 2.91%), and decanoic acid (1.23 – 1.42%). Non-saturated fatty acid; linolelaidic acid (13.51 – 16.86%), oleic acid (1.64 – 1.91%), nervonic acid (1.341.46%), dan linolenic acid (0.65 – 1.03%) measured higher as compared to the rest. Fiber components measured were NDF (20.19 - 29.11%), ADF (10.43 – 17.55%), hemicellulose (8.86 – 11.56%), cellulose (2.51 –10.49%), and Lignin (0.14 – 0.75%). Respectively, in conclusion composition of growth media of BSFL influence the profile of fatty acid and fiber in BSFL. In addition, the media composed of 50% food wastes and 50% tofu by-product gave highest and most complete fatty acid profile as well as fiber composition.