Apoferritin-camouflaged Pt nanoparticles: surface effects on cellular uptake and cytotoxicity

Abstract

Apoferritin-encapsulated Pt nanoparticles (AFt-Pt) were bio-mimetically prepared by an in situ reduction method in the unique 8 nm spherical cage of apoferritin. The average size of AFt-Pt was 4.3 AE 0.9 nm. The spherical structure of apoferritin remained intact after loading Pt particles in its cavity. Apoferritin coating increased the amount of Pt internalized in HepG2 cells by about three times via receptor-mediated endocytosis. AFt-Pt showed greatly improved biocompatibility compared with polyvinylpyrrolidone stabilized Pt nanoparticles (PVP-Pt). More than 80% of cells survived after treatment with 100 mM AFt-Pt for 48 h. By camouflaging the particles with a protein corona, apoferritin conferred a cell-recognizable identity to the particles, which strikingly ameliorated the bioeffects of the nanoparticles.

Bio-inspired protein–gold nanoconstruct with core–void–shell structure: beyond a chemo drug carrier

“…56 Native PAGE analysis of AFt, AFt-Au and AFt-AuFU was also performed as previously reported. 56 Aer gel imaging, the blue protein bands on the gel were separately cut off, digested, 57 and analyzed with ICP-AES for Au.…”

“…56 Native PAGE analysis of AFt, AFt-Au and AFt-AuFU was also performed as previously reported. 56 Aer gel imaging, the blue protein bands on the gel were separately cut off, digested, 57 and analyzed with ICP-AES for Au. The results showed that the element Au was only detected in the bands of AFt-Au and AFtAuFU while not in the band of AFt, which conrmed the successful incorporation of Au nanoparticles into apoferritin.…”

“…AFt-Au (100 mM, all the AFt-Au concentrations mentioned in this work are in terms of Au unless otherwise stated) was then added and co-incubated with the cells for 12 h. Aer that, the cells were treated according to the procedures described previously. 56 For TEM observations, sub-conuent cells were incubated with AFt-Au (100 mM) for 12 h in cell culture dishes (3.5 cm diameter). Aer that, the cells were treated according to the procedures described previously.…”

Apoferritin–CeO2nano-truffle that has excellent artificial redox enzyme activity

“…We speculated that this distinct feature of AFt-CeO 2 was partially attributed to the apoferritin corona, which could potentially promote the cellular internalization process mediated via specific ferritin receptors and prevent the adverse interactions between the nano-CeO 2 and biomolecules in the cytoplasm. 18 Further study (Fig. S6, ESIw) also suggested that the internalization of AFt-CeO 2 was primarily regulated by clathrin-mediated endocytosis (Fig.…”

Efficacy versus Toxicity - The Ying and Yang in Translating Nanomedicines

“…As an example, platinum is lately being re-examined as a nanoparticle carrier for various biological applications, including cancer [18,19]. It is well-known clinically that platinum is highly nephrotoxic [20,21], and hence one can pre-emptively predict that attempts to translate a platinum-based nanomedicinal formulations from the bench-side to the bed would pose nephro-toxicity challenges.…”