Apogamic development of plasmodia in the myxomycetePhysarum polycephalum: a cinematographic analysis

Anderson, Roger W.; Cooke, D.; Dee, Jennifer

doi:10.1007/bf01279326

Cited by 51 publications

(21 citation statements)

References 14 publications

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“…polycephalum strain CL, amoebae form plasmodia in clones (Von-Stosch et al, 1964;Wheals, 1970). The process is apogamic (Cooke & Dee, 1974;Anderson et al, 1976). CL amoebae grow 0001-4550 0 1988 SGM and differentiate on agar plates with bacteria as a food source.…”

Section: Introductionmentioning

confidence: 99%

Activation of a -Tubulin Gene during Early Development of the Plasmodium in Physarum polycephalum

1988

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Uninucleate amoebae of Physarum polycephalum strain CL undergo apogamic development to form multinucleate plasmodia via an intermediate stage of large, uninucleate cells irreversibly committed to plasmodial development. This amoebal-plasmodia1 transition involves major changes in tubulin gene expression and the organization of microtubular structures. We analysed the expression of the betC locus, which encodes the plasmodial-specific P2-tubulin, during plasmodial development. A key question addressed was the timing of expression of betC in relation to the last open mitosis of the amoeba and the first closed mitosis of the plasmodium during the transition. Culture conditions were improved to yield partly synchronous differentiating cultures containing 50-60 % committed cells, in order to facilitate biochemical analysis of development. Northern blotting indicated that betC RNA was virtually absent from amoebae and from early differentiating cultures. However, betC transcripts could already be detected in differentiating cultures containing only 0.1 % of committed cells; the relative amount of betC transcripts increased as the percentage of committed cells in differentiating cultures increased. In fully developed plasmodia, there was at least a 330-fold increase in the betC transcript level compared to that in amoebae. We conclude that betC is activated during the amoebal-plasmodia1 transition immediately before or during the commitment event. Small amounts of P2-tubulin polypeptide could first be detected by Western blotting around the stage of the first closed mitosis. Thus P2-tubulin may participate in the first closed mitosis that committed cells undergo during their development into plasmodia.

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Section: Introductionmentioning

confidence: 99%

Activation of a -Tubulin Gene during Early Development of the Plasmodium in Physarum polycephalum

1988

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“…This is accompanied by the loss of centrioles, which also results in the loss of amoebo-flagellate transformation. There are some mutant strains which omit several of these mating steps, i.e., cell fusion, karyogamy and formation of diploid cell nuclei, by forming plasmodia directly from a single amoeba genotype (Anderson et al 1976, Cooke andDee 1974). This phenomenon is known as amoebal-plasmodial transition as opposed to plasmodium formation through mating.…”

Section: Discussionmentioning

confidence: 99%

Non-synchronous Degradation of Centrioles during Mating in Physarum polycephalum.

et al. 1994

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“…Instead, the developing uninucleate cell continues to grow for a period about 2.3-times as long as a normal amoebal cell cycle. At the end of this extended cell cycle, the uninucleate cell is twice as large as an amoeba at mitosis and becomes binucleate by mitosis without cytokinesis (Anderson et al, 1976;Bailey e t al., 1987). About halfway through the extended cell cycle, the developing cell becomes committed to development and loses the ability to undergo the amoeba-flagellate transformation (Fig.…”

Section: Apogamic Developmentmentioning

confidence: 99%

Plasmodium development in the myxomycete Physarum polycephalum: genetic control and cellular events

Bailey

1995

Microbiology

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Apogamic development of plasmodia in the myxomycetePhysarum polycephalum: a cinematographic analysis

Cited by 51 publications

References 14 publications

Activation of a -Tubulin Gene during Early Development of the Plasmodium in Physarum polycephalum

Activation of a -Tubulin Gene during Early Development of the Plasmodium in Physarum polycephalum

Non-synchronous Degradation of Centrioles during Mating in Physarum polycephalum.

Plasmodium development in the myxomycete Physarum polycephalum: genetic control and cellular events

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