Apolipoprotein A-I modulates HDL particle size in the absence of apolipoprotein A-II

Melchior, John T.; Street, Scott E.; Vaisar, Tomáš; Hart, Rachel C; Jerome, Jay; Kuklenyik, Zsuzsanna; Clouet-Foraison, Noémie; Thornock, Carissa; Bedi, Shimpi; Shah, Amy S.; Segrest, Jere P.; Heinecke, Jay W.; Davidson, W. Sean

doi:10.1016/j.jlr.2021.100099

Cited by 18 publications

(8 citation statements)

References 61 publications

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“…HDLs contain more than 250 different proteins and upward of 200 different species of neutral or polar lipids ( 3 , 33 ). To determine whether the antapoptotic effect of HDL was due to the protein or the lipid component, we took advantage of our ability to selectively incorporate various HDL protein and lipid components into rHDL particles ( 26 , 34 ). First, we evaluated the protein component of HDL by delipidating HDL under conditions that preserve the protein components ( 23 ).…”

Section: Resultsmentioning

confidence: 99%

Apolipoprotein E-containing HDL decreases caspase-dependent apoptosis of memory regulatory T lymphocytes

Atehortua,

Morris,

Street

et al. 2023

Journal of Lipid Research

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Section: Resultsmentioning

confidence: 99%

Apolipoprotein E-containing HDL decreases caspase-dependent apoptosis of memory regulatory T lymphocytes

Atehortua,

Morris,

Street

et al. 2023

Journal of Lipid Research

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“…APOA1 and APOA2 are the most abundant with the ability to be included in multiple copies in each particle 47 . Their levels have been shown to be estrogen-responsive; increasing with postmenopausal administration of estrogen therapy and rapidly decreasing after its cessation 50 .…”

Section: Discussionmentioning

confidence: 99%

Proteomics and lipidomics of high-density lipoprotein: Perimenopause is characterized by small triacylglycerols-enriched particles

Lehti,

Korhonen,

Soliymani

et al. 2024

Preprint

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Menopause is associated with a proatherogenic shift in serum metabolome and high-density lipoprotein (HDL) particle size distribution. We analyzed lipidomes and proteomes of HDL with nuclear magnetic resonance and mass spectrometry from pre-, peri-, and postmenopausal women to get a deeper insight into the structure of HDL. The S-HDL particles constituted 62% of all HDL particles in perimenopause and 60% in pre- and postmenopause, thus, perimenopausal HDL had the highest S-HDL lipid content, notably, being enriched in triacylglycerols. This feature is a known risk factor for coronary heart disease. We identified 728 proteins from the purified HDL particles and quantified 44 representing functional classes of lipid metabolism, transport and signaling, immune defense, and regulation of cellular processes. Perimenopausal HDL exhibited fewer apolipoproteins (APOA1, APOA2, APOC1, APOC3, and APOE) per particle than premenopausal. We did not detect menopausal status-associated deteriorations in the LCAT activity or cholesterol efflux capacity, albeit the calculated lipid class ratios suggest defects, especially within PERI XL-HDL particles, potentially affecting the particle size distribution and triacylglycerol content. In summary, menopause is associated with structural differences in HDL potentially compromising the cardioprotective quality of HDL.

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“…Apoa2 is the main component protein in human HDL particles, and unlike apoAI and the negative association with atherosclerosis, the biological and physiological functions of Apoa2 are largely unknown. 45 Currently, research suggests that Apoa2 over-expression is associated with an increased risk of cardiovascular disease. Over-expression of Apoa2 has been associated with insulin resistance and exacerbation of atheromatous plaques in animal studies, with the possible mechanism being that cysteinylation of the monomeric chain regulates Apoa2 dimerization and increased HDL-C levels.…”

Section: Discussionmentioning

confidence: 99%

Effects of Semaglutide on Cardiac Protein Expression and Cardiac Function of Obese Mice

Pan¹,

Yue²,

Ban³

et al. 2022

JIR

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Using proteomics to study the effect of semaglutide on cardiac protein expression in obese mice. Assessment of the effect of semaglutide on cardiac function in obese mice. Materials and Methods:The mice were randomly divided into three groups: the control group (WC), the high-fat group (WF), and the high-fat diet with semaglutide intervention group (WS). Serum samples were collected, and lipids, blood glucose, inflammatory and oxidative stress markers, and cardiac ultrasound, were examined. The cardiac weight of each group of mice was measured, and pathological alterations were examined. Inflammation and oxidative stress levels in heart tissue were evaluated. The labeling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform was used to find differentially expressed proteins (DEPs) and screen for related pathways and key proteins in a proteomics study. Results: Semaglutide greatly alleviated obesity-induced lipid metabolism abnormalities, improved cardiac ventricular wall thickening, and significantly reduced myocardial collagen content in obese mice. Semaglutide significantly reduces obesity-induced inflammation and oxidative stress. There were 64 DEPs in the WF/WC group, with 39 upregulated proteins and 25 downregulated proteins. The WS/ WC group, on the other hand, had 83 DEPs, including 57 upregulated and 26 downregulated proteins. Following functional analysis, DEPs were shown to be largely associated with lipid metabolism and peroxisomes. Apolipoprotein A-II, catalase, diazepam-binding inhibitor, paraoxonase-1, and hydroxysteroid 17-dehydrogenase-4 were all upregulated in the WF group but significantly downregulated in the WS group. A high-fat diet increases the expression of lipid synthesis and transport proteins while increasing inflammation and oxidative stress damage. Conclusion: Semaglutide decreases lipid synthesis alleviates inflammation and oxidative stress and prevents lipid peroxidation and cardiac impairment.

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Apolipoprotein A-I modulates HDL particle size in the absence of apolipoprotein A-II

Cited by 18 publications

References 61 publications

Apolipoprotein E-containing HDL decreases caspase-dependent apoptosis of memory regulatory T lymphocytes

Apolipoprotein E-containing HDL decreases caspase-dependent apoptosis of memory regulatory T lymphocytes

Proteomics and lipidomics of high-density lipoprotein: Perimenopause is characterized by small triacylglycerols-enriched particles

Effects of Semaglutide on Cardiac Protein Expression and Cardiac Function of Obese Mice

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