Apolipoprotein C1 stimulates the malignant process of renal cell carcinoma via the Wnt3a signaling

Abstract

Background Renal cell carcinoma (RCC) is a clinically common tumor in the urinary system, showing an upward trend of both incidence and mortality. Apolipoprotein C1 (APOC1) has been identified as a vital regulator in tumor progression. This study aims to uncover the biological function of APOC1 in RCC process and the underlying mechanism. Methods Differential levels of APOC1 in RCC samples and normal tissues in a downloaded TCGA profile and clinical samples collected in our center were detected by quantitative reverse transcription PCR (qRT-PCR). The prognostic value of APOC1 in RCC was assessed by depicting Kaplan–Meier survival curves. After intervening APOC1 level by transfection of sh-APOC1 or oe-APOC1, changes in phenotypes of RCC cells were examined through CCK-8, colony formation, Transwell assay and flow cytometry. Subsequently, protein levels of EMT-related genes influenced by APOC1 were determined by Western blot. The involvement of the Wnt3a signaling in APOC1-regulated malignant process of RCC was then examined through a series of rescue experiments. Finally, a RCC xenograft model was generated in nude mice, aiming to further clarify the in vivo function of APOC1 in RCC process. Results APOC1 was upregulated in RCC samples. Notably, its level was correlated to overall survival of RCC patients, displaying a certain prognostic value. APOC1 was able to stimulate proliferative, migratory and invasive abilities in RCC cells. The Wnt3a signaling was identified to be involved in APOC1-mediated RCC process. Notably, Wnt3a was able to reverse the regulatory effects of APOC1 on RCC cell phenotypes. In vivo knockdown of APOC1 in xenografted nude mice slowed down the growth of RCC. Conclusions APOC1 stimulates the malignant process of RCC via targeting the Wnt3a signaling.

The expression and clinical significance of apolipoprotein C1 in cervix cancer

“…In this study, we found that APOC1 was highly expressed in CC patients at both the mRNA and protein level, as determined by bioinformatics data mining and comprehensive tissue anal-ysis; these findings concur with previous literature [24][25][26][27][28].…”

APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN

“…Research revealed that APOC1 plays a role in the pathogenesis of glomerulosclerosis ( Bus et al, 2017 ). Additionally, APOC1 represents a novel diagnostic marker for clear cell renal cell carcinoma ( Cui et al, 2020 ) and stimulates renal cell carcinoma through Wnt3a/STAT signaling ( Li et al, 2020 ; Jiang et al, 2021 ). APOC1 is involved in breast cancer progression via the EMT and MAPK/JNK pathway ( Zhang et al, 2022b ).…”

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