Apolipoprotein E genotype is a determinant of low-density lipoprotein cholesterol and of its response to a low-cholesterol diet in Type 1 diabetic patients with elevated urinary albumin excretion

Blaauwwiekel, EE; Beusekamp, BJ; Sluiter, Willem; Hoogenberg, Klaas; Dullaart, Robin P. F.

doi:10.1002/(sici)1096-9136(1998120)15:12<1031::aid-dia705>3.0.co;2-6

Cited by 16 publications

(5 citation statements)

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“…Plasma total cholesterol, triglycerides, HDL cholesterol, Apo A-I, ApoB and ApoE were measured as previously described [28–30,44,45]. ApoE genotyping was performed as described previously [52]. Non-HDL cholesterol was calculated as the difference between total cholesterol and HDL cholesterol.…”

Section: Methodsmentioning

confidence: 99%

Plasma ApoE elevations are associated with NAFLD: The PREVEND Study

et al. 2019

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Non-alcoholic fatty liver disease (NAFLD) is featured by increased plasma very low density lipoproteins (VLDL). The extent to which plasma apolipoprotein E (ApoE) levels are elevated in NAFLD is unclear. We determined whether plasma ApoE is elevated in subjects with suspected NAFLD. Plasma ApoE and genotypes were determined in 6,762 participants of the Prevention of Renal and Vascular End-Stage Disease (PREVEND) cohort. A Fatty Liver Index (FLI) ≥ 60 was used as a proxy of NAFLD. A total of 1,834 participants had a FLI ≥ 60, which coincided with increased triglycerides, non-HDL cholesterol, ApoB and ApoE (all P <0.001). In multivariable linear regression analysis, plasma ApoE levels were positively associated with an elevated FLI when taking account of ApoE genotypes and other clinical and laboratory covariates (fully adjusted model: β = 0.201, P <0.001). Stratified analysis for ApoE genotypes (ApoE ε3ε3 homozygotes, ApoE ε2 carriers, and ApoE ε3ε4 and ε4ε4 carriers combined), also showed positive associations of plasma ApoE levels with an elevated FLI in each group (all P <0.001). In conclusion, it is suggested that NAFLD is characterized by increased plasma ApoE levels, even when taking account of the various ApoE genotypes. Increased plasma ApoE may contribute to altered VLDL metabolism and to increased atherosclerosis susceptibility in NAFLD.

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Section: Methodsmentioning

confidence: 99%

Plasma ApoE elevations are associated with NAFLD: The PREVEND Study

et al. 2019

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“…To this end DNA was extracted from whole blood using the Qiampmini kit (Qiagen). The method has been validated against a previously described restriction isotyping procedure [26,27].…”

Section: Laboratory Measurementsmentioning

confidence: 99%

Higher Plasma ApoE Levels are Associated with Low-Normal Thyroid Function: Studies in Diabetic and Nondiabetic Subjects

2016

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Low-normal thyroid function within the euthyroid range may confer higher plasma triglycerides, but relationships with plasma apolipoprotein (apo) E, which plays an important role in the metabolism of triglyceride-rich apoB-containing lipoproteins, are unknown. We determined relationships of plasma apoE with thyroid stimulating hormone (TSH) and free thyroxine (free T4) in euthyroid subjects with and without Type 2 diabetes mellitus (T2DM). TSH, free T4, lipids, and apoE were measured in fasting plasma from 72 T2DM subjects and 82 nondiabetic subjects. The APOE genotype was also determined. Free T4 was slightly higher in T2DM (p=0.030), but TSH levels were not different vs. nondiabetic subjects. The APOE genotype distribution was not different between the groups. None of the participants had the ε2/ε2 genotype. Plasma triglycerides were higher in T2DM (p=0.037). ApoB and apoE levels were not different between the groups. In all subjects combined, multivariable analysis showed that plasma triglycerides (p=0.039), non-high density lipoprotein (non-HDL) cholesterol (p=0.030), and apoE levels (p=0.002) were each independently and positively associated with TSH after adjustment for age, sex, T2DM and the presence of the APOE ε3 allele. Furthermore, the associations of TSH with apoE remained present after adjustment for either triglycerides, non-HDL cholesterol, or apoB (p=0.005 to 0.023). The presence of T2DM did not modify the relationships of TSH with these (apo) lipoprotein variables (p=0.11 to 0.36). In conclusion, low-normal thyroid function, as indicated by higher TSH levels within the euthyroid range, may influence the metabolism of triglyceride-rich lipoproteins by affecting apoE regulation.

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“…However, replacement of saturated fat by carbohydrates not only lowers plasma LDL cholesterol, but may decrease plasma high-density lipoprotein (HDL) cholesterol levels as well [3][4][5][6]. A reduction in cholesterol intake also decreases plasma HDL cholesterol in non-diabetic [7] as well as in type 1 diabetic individuals [8].…”

Section: Introductionmentioning

confidence: 99%

A low‐saturated‐fat, low‐cholesterol diet decreases plasma CETP activity and pre β‐HDL formation but does not affect cellular cholesterol efflux to plasma from type 1 diabetic patients

Vries

Beusekamp

Kerstens

et al. 2005

Scandinavian Journal of Clinical and Laboratory Investigation

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The aim of this study was to evaluate the effect of a low-saturated-fat, low-cholesterol diet on plasma lipopoproteins, pre beta-high density lipoprotein (HDL) formation, lecithin:cholesterol acyltransferase (LCAT), cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) activities, as well as on the ability of plasma to stimulate cellular cholesterol efflux. Twelve male type 1 diabetic patients with plasma cholesterol >5.0 mmol/L were studied while consuming their usual diet and after 6 weeks of a low-fat, low-cholesterol diet. Pre beta-HDL formation was measured using crossed immuno-electrophoresis. Plasma LCAT, CETP and PLTP activities were assayed by exogenous substrate methods. The ability of plasma to promote cellular cholesterol efflux out of Fu5AH rat hepatoma cells and out of human skin fibroblasts was also determined. Saturated fat intake was lowered (p = 0.001) due to replacement with carbohydrates, while mono- and polyunsaturated fat intake remained unchanged. Cholesterol intake decreased as well (p = 0.003). The changes in plasma total cholesterol, very low and low-density lipoprotein (VLDL+LDL) cholesterol, HDL cholesterol, HDL phospholipids, apolipoprotein (apo) A-I, plasma LCAT activity and PLTP activity were not significant. Plasma CETP activity (p = 0.008) and pre beta-HDL formation (p = 0.008) decreased. The ability of plasma to promote cholesterol efflux out of fibroblasts and Fu5AH cells remained unchanged. Reduction in dietary saturated fat and cholesterol intake does not adversely affect cellular cholesterol efflux to plasma from type 1 diabetic patients, despite a drop in pre beta-HDL formation.

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Apolipoprotein E genotype is a determinant of low-density lipoprotein cholesterol and of its response to a low-cholesterol diet in Type 1 diabetic patients with elevated urinary albumin excretion

Cited by 16 publications

References 0 publications

Plasma ApoE elevations are associated with NAFLD: The PREVEND Study

Plasma ApoE elevations are associated with NAFLD: The PREVEND Study

Higher Plasma ApoE Levels are Associated with Low-Normal Thyroid Function: Studies in Diabetic and Nondiabetic Subjects

A low‐saturated‐fat, low‐cholesterol diet decreases plasma CETP activity and pre β‐HDL formation but does not affect cellular cholesterol efflux to plasma from type 1 diabetic patients

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