Apomixis and Gene Expression in <i>Boechera</i>

Hofmann, Nancy R.

doi:10.1105/tpc.110.220312

Cited by 11 publications

(9 citation statements)

References 4 publications

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“…However, there was no obvious developmental pathway or timing change that could simply explain the shift to apomixis. Transcription factors were overrepresented among apomixis-specific genes, suggestive of large-scale regulatory changes in apomictic ovules (Hofmann, 2010;Sharbel et al, 2010).…”

Section: Discussion Apomixis Control and Transcriptional Analyses Of mentioning

confidence: 99%

Enlarging Cells Initiating Apomixis inHieracium praealtumTransition to an Embryo Sac Program prior to Entering Mitosis

Okada

Hu²,

Tucker³

et al. 2013

Plant Physiology

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Hieracium praealtum forms seeds asexually by apomixis. During ovule development, sexual reproduction initiates with megaspore mother cell entry into meiosis and formation of a tetrad of haploid megaspores. The sexual pathway ceases when a diploid aposporous initial (AI) cell differentiates, enlarges, and undergoes mitosis, forming an aposporous embryo sac that displaces sexual structures. Embryo and endosperm development in aposporous embryo sacs is fertilization independent. Transcriptional data relating to apomixis initiation in Hieracium spp. ovules is scarce and the functional identity of the AI cell relative to other ovule cell types is unclear. Enlarging AI cells with undivided nuclei, early aposporous embryo sacs containing two to four nuclei, and random groups of sporophytic ovule cells not undergoing these events were collected by laser capture microdissection. Isolated amplified messenger RNA samples were sequenced using the 454 pyrosequencing platform and comparatively analyzed to establish indicative roles of the captured cell types. Transcriptome and protein motif analyses showed that approximately one-half of the assembled contigs identified homologous sequences in Arabidopsis (Arabidopsis thaliana), of which the vast majority were expressed during early Arabidopsis ovule development. The sporophytic ovule cells were enriched in signaling functions. Gene expression indicative of meiosis was notably absent in enlarging AI cells, consistent with subsequent aposporous embryo sac formation without meiosis. The AI cell transcriptome was most similar to the early aposporous embryo sac transcriptome when comparing known functional annotations and both shared expressed genes involved in gametophyte development, suggesting that the enlarging AI cell is already transitioning to an embryo sac program prior to mitotic division.

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Section: Discussion Apomixis Control and Transcriptional Analyses Of mentioning

confidence: 99%

Enlarging Cells Initiating Apomixis inHieracium praealtumTransition to an Embryo Sac Program prior to Entering Mitosis

Okada

Hu²,

Tucker³

et al. 2013

Plant Physiology

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“…As a result of this favorable characteristic, an intense interest has been focused on the issue of how to successfully engineer it in a wide range of commercial crops. A considerable body of molecular studies has been made to identify key genes and developmental pathways that enable cells in the ovule to switch to an apomictic channel [2,3,[8][9][10][11][12][13][14]. All these aim to afford the best opportunity to produce maternally derived, genetically identical seeds in flowering plants.…”

Section: Discussionmentioning

confidence: 99%

“…As a natural way of circumventing genetic segregation and maintaining heterosis in a hybrid progeny, apomixis has been widely used as a genetic tool for plant breeding [4,5]. To engineer apomixis into crops [5][6][7], a systematic study has been made to understand the genetic control of this phenomenon [8,9] and its underlying regulatory pathways [2] and gene expression differentiation [10][11][12][13][14]. More recently, the use of linkage analysis to map and identify individual genes that control apomixis has been increasingly recognized [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

A statistical design for testing apomictic diversification through linkage analysis

Zeng

Hou

Song

et al. 2012

Briefings in Bioinformatics

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The capacity of apomixis to generate maternal clones through seed reproduction has made it a useful characteristic for the fixation of heterosis in plant breeding. It has been observed that apomixis displays pronounced intra- and interspecific diversification, but the genetic mechanisms underlying this diversification remains elusive, obstructing the exploitation of this phenomenon in practical breeding programs. By capitalizing on molecular information in mapping populations, we describe and assess a statistical design that deploys linkage analysis to estimate and test the pattern and extent of apomictic differences at various levels from genotypes to species. The design is based on two reciprocal crosses between two individuals each chosen from a hermaphrodite or monoecious species. A multinomial distribution likelihood is constructed by combining marker information from two crosses. The EM algorithm is implemented to estimate the rate of apomixis and test its difference between two plant populations or species as the parents. The design is validated by computer simulation. A real data analysis of two reciprocal crosses between hickory (Carya cathayensis) and pecan (C. illinoensis) demonstrates the utilization and usefulness of the design in practice. The design provides a tool to address fundamental and applied questions related to the evolution and breeding of apomixis.

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“…Most importantly, apomixis can be used for the fixation of hybrid vigor in crops where heterosis has been exploited to develop F1 hybrid varieties (Singh et al 2007;Spillane et al 2004). Therefore, the ability to induce apomixis in crop plants has been referred to as a holy grail of plant biology (Hofmann 2010). Apomixis occurs in many wild species and in a few agronomically important species, such as citrus and mango, but not in any of the major cereal crops (Eckardt 2003).…”

Section: Introductionmentioning

confidence: 99%

Construction of a Full-Length cDNA Library and Analysis of Expressed Sequence Tags from Inflorescence of Apomictic Sabaigrass (Eulaliopsis binata)

Zhang

Ouyang

et al. 2011

Plant Mol Biol Rep

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Eulaliopsis binata, which is a close relative of cereal crops, was recognized as an important research material owing to its high frequency of apospory and autonomous endosperm formation. However, little information is known about its genomics and regulatory pathway participating in reproductive development. For the first step to understand molecular basis in sabaigrass (E. binata), a SMART complementary DNA library from the inflorescence tissue was constructed and characterized. The titers of original and amplified libraries were 5.53×10 6 and 1.49×10 10 pfu/ml, respectively. The percentage of recombinants was 96% in the original library. Analysis of sequencing results of 398 out of 437 randomly picked clones showed that 271 (68.1%) expressed sequence tags (ESTs) exhibited significant similarity with known putative functional nucleotide sequences in the GenBank databases, 25 (6.3%) ESTs have significant matches with hypothetical proteins, putative proteins, and unknown proteins, and the other 25.6% ESTs had no significant similarity to sequences in the public databases. Based on molecular function of GO annotation, the four most abundant terms are nucleotide binding, hydrolase activity, ion binding, and protein binding, and these genes were involved in 61 different pathways using the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Besides, simple sequence repeats detection in 398 ESTs was carried out, and several genes were chosen to perform expression analysis. This report represents a first step in expanding molecular-genetic analyses in E. binata and can be used to optimally mine useful information from a relatively small data set.

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Apomixis and Gene Expression in Boechera

Cited by 11 publications

References 4 publications

Enlarging Cells Initiating Apomixis inHieracium praealtumTransition to an Embryo Sac Program prior to Entering Mitosis

Enlarging Cells Initiating Apomixis inHieracium praealtumTransition to an Embryo Sac Program prior to Entering Mitosis

A statistical design for testing apomictic diversification through linkage analysis

Construction of a Full-Length cDNA Library and Analysis of Expressed Sequence Tags from Inflorescence of Apomictic Sabaigrass (Eulaliopsis binata)

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