Apoplast Acidification in Growing Barley (Hordeum vulgare L.) Leaves

Visnovitz, Tamás; Touati, Mostefa; Miller, Anthony J.; Fricke, Wieland

doi:10.1007/s00344-012-9282-8

Cited by 9 publications

(9 citation statements)

References 154 publications

(290 reference statements)

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“…However, a comparative quantitative analysis of the effects of IAA and the fungal phytotoxin Fusicoccin (FC) on growth and proton secretion in coleoptiles of maize and oats ( Avena sativa ) led to the conclusion that FC, but not IAA, causes wall-loosening and cell enlargement via a rapid acidification of the cell walls. This “acid-growth theory of FC-action”, which is compatible with the “expansin-concept of wall-loosening”, is well supported by numerous independent lines of evidence (Schopfer 1993; Kutschera 1994, 2001, 2003, 2006; Karcz and Burdach 2007; Niklas and Kutschera 2012; Visnovitz et al 2013; Burdach et al 2014; Rudnicka et al 2014).…”

Section: Coleoptile Elongation: Acid Growth and Osmiophilic Nanopartisupporting

confidence: 61%

Growth-limiting proteins in maize coleoptiles and the auxin-brassinosteroid hypothesis of mesocotyl elongation

Kutschera

Wang

2015

Protoplasma

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The shoot of grass coleoptiles consists of the mesocotyl, the node, and the coleoptile (with enclosed primary leaf). Since the 1930s, it is known that auxin (indole-3-acetic acid, IAA), produced in the tip of the coleoptile, is the central regulator of turgor-driven organ growth. Fifty years ago, it was discovered that antibiotics that suppress protein biosynthesis, such as cycloheximide, inhibit auxin (IAA)-induced cell elongation in excised sections of coleoptiles and stems. Based on such inhibitor studies, the concept of "growth-limiting proteins (GLPs)" emerged that was subsequently elaborated and modified. Here, we summarize the history of this idea with reference to IAA-mediated shoot elongation in maize (Zea mays) seedlings and recent studies on the molecular mechanism underlying auxin action in Arabidopsis thaliana. In addition, the analysis of light-induced inhibition of shoot elongation in intact corn seedlings is discussed. We propose a concept to account for the GLP-mediated epidermal wall-loosening process in coleoptile segments and present a more general model of growth regulation in intact maize seedlings. Quantitative proteomic and genomic studies led to a refinement of the classic "GLP concept" to explain phytohormone-mediated cell elongation at the molecular level (i.e., the recently proposed theory of a "central growth regulation network," CGRN). Novel data show that mesocotyl elongation not only depends on auxin but also on brassinosteroids (BRs). However, the biochemical key processes that regulate the IAA/BR-mediated loosening of the expansion-limiting epidermal wall(s) have not yet been elucidated.

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Section: Coleoptile Elongation: Acid Growth and Osmiophilic Nanopartisupporting

confidence: 61%

Growth-limiting proteins in maize coleoptiles and the auxin-brassinosteroid hypothesis of mesocotyl elongation

Kutschera

Wang

2015

Protoplasma

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“…The potassium concentration in the nutrient solution was 2 mM. Plants were exposed to salt stress (no incremental increase in salt) when they were 10-11 d old and analysed when they were 14-17 d old, after a minimum exposure to salt for 4 d. At this developmental stage, leaf two provided the bulk of photosynthesizing leaf area (≥ 50%) and leaf three was elongating at maximum and steady rates (Fricke & Peters, 2002;Visnovitz et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

“…Shoot and root surface area (scanned images, analysed using IMAGEJ software (Even et al, 2018) and FW of plants were determined following the completion of transpiration measurements. Data on the cytosolic concentrations of solutes and membrane potentials at the plasma membrane and tonoplast were taken from electrophysiological studies on leaves of control-grown and salt-stressed barley (Carden et al, 2001(Carden et al, , 2003Cuin et al, 2003;Visnovitz et al, 2013; see Notes S1); data on root cells were used where data on leaf cells were lacking. Given this approach, it is inevitable that some approximations needed to be made.…”

Section: Methodsmentioning

confidence: 99%

Energy costs of salinity tolerance in crop plants: night‐time transpiration and growth

Fricke

2019

New Phytologist

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Summary Plants grow and transpire during the night. The aim of the present work was to assess the relative flows of carbon, water and solutes, and the energy involved, in sustaining night‐time transpiration and leaf expansive growth under control and salt‐stress conditions. Published and unpublished data were used, for barley plants grown in presence of 0.5–1 mM NaCl (control) and 100 mM NaCl. Night‐time leaf growth presents a more efficient use of taken‐up water compared with day‐time growth. This efficiency increases several‐fold with salt stress. Night‐time transpiration cannot be supported entirely through osmotically driven uptake of water through roots under salt stress. Using a simple three‐ (root medium/cytosol/vacuole) compartment approach, the energy required to support cell expansion during the night is in the lower percentage region (0.03–5.5%) of the energy available through respiration, under both, control and salt‐stress conditions. Use of organic (e.g. hexose equivalents) rather than inorganic (e.g. Na+, Cl−, K+) solutes for generation of osmotic pressure in growing cells, increases the energy demand by orders of magnitude, yet requires only a small portion of carbon assimilated during the day. Night‐time transpiration and leaf expansive growth should be considered as a potential acclimation mechanism to salinity.

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“…The proton mobilization is a process of importance contributing to the pH homeostasis inside or outside of the cell. Proton fluxes were shown, for example, in the pH changes monitored by pH-sensitive fluorescent probes after the induction of variation potential in pumpkin seedlings (Sherstneva et al, 2015), and also in the determination of apoplast acidification in barley leaves using pH-microelectrode (Visnovitz et al, 2013). The advantage of the method used here was to comparatively quantify the amount of protons mobilized following the different treatments.…”

Section: C2 and C3 Induced Very Early Modifications On Ionic Equilibriummentioning

confidence: 95%

Unsaturated amino acids derived from isoleucine trigger early membrane effects on plant cells

Roblin

Laduranty

Bonmort

et al. 2016

Plant Physiology and Biochemistry

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Unsaturated amino acids (UnsAA) have been shown to affect the activity of various biological processes. However, their mode of action has been investigated poorly thus far. We show in this work that 2-amino-3-methyl-4-pentenoic acid (C2) and 2-amino-3-methyl-4-pentynoic acid (C3) structurally derived from isoleucine (Ile) exhibited a multisite action on plant cells. For one, C2 and C3 induced early modifications at the plasma membrane level, as shown by the hyperpolarization monitored by microelectrode implantation in the pulvinar cells of Mimosa pudica, indicating that these compounds are able to modify ionic fluxes. In particular, proton (H(+)) fluxes were modified, as shown by the pH rise monitored in the bathing medium of pulvinar tissues. A component of this effect may be linked to the inhibitory effect observed on the proton pumping and the vanadate-sensitive activity of the plasma membrane H(+)-ATPase monitored in plasma membrane vesicles (PMVs) purified from pulvinar tissues of M. pudica and leaf tissues of Beta vulgaris. This effect may explain, in part, the inhibitory effect of the compounds on the uptake capacity of sucrose and valine by B. vulgaris leaf tissues. In contrast, an unexpected action was observed in cell reactions, implicating ion fluxes and water movement. Indeed, the osmocontractile reactions of pulvini induced either by a mechanical shock in M. pudica or by dark and light signals in Cassia fasciculata were increased, indicating that, compared to Ile, these compounds may modify in a specific way the plasma membrane permeability to water and ions.

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Apoplast Acidification in Growing Barley (Hordeum vulgare L.) Leaves

Abstract: ________ x Statement of Original AuthorshipxiCollaborations xii Acknowledgements xiii

Cited by 9 publications

References 154 publications

Growth-limiting proteins in maize coleoptiles and the auxin-brassinosteroid hypothesis of mesocotyl elongation

Growth-limiting proteins in maize coleoptiles and the auxin-brassinosteroid hypothesis of mesocotyl elongation

Energy costs of salinity tolerance in crop plants: night‐time transpiration and growth

Unsaturated amino acids derived from isoleucine trigger early membrane effects on plant cells

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