Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies

López, Lidia M.; Dubin, Marta; Carrizo, Patricia H.; Grande, María José López; Iraldi, Amanda Pellegrino de; Stoppani, A. O. M.

doi:10.32604/biocell.2003.27.213

Cited by 6 publications

(1 citation statement)

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“…Nevertheless, hepatocytes contain powerful antioxidant systems such as the glutathione peroxidase system and catalase, which remained unaffected under our experimental conditions (data not shown), and thus able to limit the quinone-generated reactive species so they do not reach the level to induce cell death. It is worth mentioning that after a 4 h-incubation with CGQ no significant decrease of cell viability was observed, which, however, does not exclude that cell death by apoptosis could be starting [52]. Since CGQ inhibited lipid peroxidation, no significant role could be attributed to lipid peroxidation products as intermediates of CGQ action.…”

Section: Discussionmentioning

confidence: 88%

The metabolism of 9-chloro-β-lapachone and its effects in isolated hepatocytes. The involvement of NAD(P)H:quinone oxidoreductase 1 (NQO1)

Villamil¹,

Carrizo²,

Rosso³

et al. 2012

Chemico-Biological Interactions

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Section: Discussionmentioning

confidence: 88%

The metabolism of 9-chloro-β-lapachone and its effects in isolated hepatocytes. The involvement of NAD(P)H:quinone oxidoreductase 1 (NQO1)

Villamil¹,

Carrizo²,

Rosso³

et al. 2012

Chemico-Biological Interactions

Self Cite

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ICAT as a potential enhancer of monocytic differentiation: implications from the comparative proteome analysis of the HL60 cell line stimulated by all‐trans retinoic acid and NSC67657

Wang

Zhang

Deng

et al. 2009

Cell Biochemistry & Function

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A novel sterol mesylate compound (NSC67657) was recently identified and reported by National Cancer Institute that could efficiently induce the differentiation of HL60 cells into monocytes in vitro and in vivo. The expression of many proteins would have been changed during the differentiation process, and some proteins may have played key roles in the differentiation of HL60 cell line induced by this drug. Therefore, we treated HL60 cells with NSC67657 and all-trans retinoic acid (ATRA) to identify the differentially expressed proteins and determine their functions in cellular differentiation. Of the 45 differentially expressed protein spots investigated, 24 were either elevated or decreased in both the monocytic and granulocytic differentiating HL60 cells, 8 showed significant changes only when induced by NSC67657, and 13 showed significant changes only when induced by ATRA. After verification by RT-PCR, Western blotting, and immunocytochemistry, only the protein ICAT was found to be elevated by NSC67657 treatment alone. Although the over-expression of ICAT is not sufficient to induce the differentiation of HL60 cells into monocytes, it did increase the proportion of CD14+ cells in cells pretreated with NSC67657. Successful application of multiple techniques including two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Western blotting, and eukaryotic electroporation revealed that proteomic and molecular biological analyses provide valuable tools in drug development research.

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Fish oil prevents colon cancer by modulation of structure and function of mitochondria

Agnihotri

Sharma

Rani

et al. 2016

Biomedicine & Pharmacotherapy

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Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies

Cited by 6 publications

References 0 publications

The metabolism of 9-chloro-β-lapachone and its effects in isolated hepatocytes. The involvement of NAD(P)H:quinone oxidoreductase 1 (NQO1)

The metabolism of 9-chloro-β-lapachone and its effects in isolated hepatocytes. The involvement of NAD(P)H:quinone oxidoreductase 1 (NQO1)

ICAT as a potential enhancer of monocytic differentiation: implications from the comparative proteome analysis of the HL60 cell line stimulated by all‐trans retinoic acid and NSC67657

Fish oil prevents colon cancer by modulation of structure and function of mitochondria

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