Apoptosis and astrogliosis perturbations and expression of regulatory inflammatory factors and neurotransmitters in acrylamide-induced neurotoxicity under ω3 fatty acids protection in rats

Elblehi, Samar S.; Euony, Omnia I. El; El‐Sayed, Yasser S.

doi:10.1016/j.neuro.2019.10.004

Cited by 39 publications

(19 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, a study on the immune function of mice reported that acrylamide could induce immunotoxicity via decreas- ing the GSH concentration (and increasing lipid peroxidation) in the spleen of mice (43). Furthermore, the role of oxidative stress in the acrylamide induced toxicity is proved in other studies (35)(36)(37). However, carvacrol could increase the level of GSH in cells treated with acrylamide or H 2 O 2 , which is in compliance with other experimental studies (37)(38)(39).…”

Section: Discussionsupporting

confidence: 75%

“…It has also been suggested that oxygen-free radicals play a key role in brain senescence due to the elevated levels of MDA found in the brain of aged mice (34). In this study, acrylamide could increase the level of lipid peroxidation, which is attributed to the oxidative stress-inducing properties of this compound (35,36). Furthermore, carvacrol treatment effectively reduced the level of lipid peroxidation in the acrylamide-and H 2 O 2 -treated cell groups.…”

Section: Discussionmentioning

confidence: 50%

See 1 more Smart Citation

Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol

Evazalipour

Kozani

et al. 2021

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

Background: Stress-induced cellular senescence is a perpetual state of cell cycle arrest occurring in proliferating cells in response to stressful conditions. It is believed that oxidative stress plays a unique role in this process. As a reactive chemical compound that can induce oxidative stress, acrylamide is widely applied in several fields. Carvacrol is a liquid phenolic monoterpenoid found in essential oils of some plants and is known for its antioxidant and anticarcinogenic properties. Objectives: The current study aimed to evaluate the effects of carvacrol on oxidative stress and cellular senescence induced by acrylamide in the NIH 3T3 cell line. Methods: NIH 3T3 embryonic fibroblast cells were exposed to different concentrations of acrylamide, carvacrol, and H2O2 in a cell culture medium. The level of β-galactosidase (SA-β-gal) activity, as a marker of cellular senescence, was measured using staining and quantitative assays. Furthermore, to measure oxidative stress parameters, the content of glutathione and lipid peroxidation were determined. Results: Acrylamide could induce premature senescence evident by the elevated lipid peroxidation and SA-β-gal activity and declined cell viability and glutathione. Moreover, carvacrol showed beneficial effects on both acrylamide- and H2O2-induced cellular senescence by significantly reversing or subsiding the effect of oxidative stress and mediating its consequences. Conclusions: It can be concluded that carvacrol has protective effects against oxidative cellular senescence induced by acrylamide in the NIH 3T3 cell line.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 50%

Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol

Evazalipour

Kozani

et al. 2021

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

show abstract

“…Using images of immunostained slides, the area percentage (%) of TGF-β1, cleaved caspase-3, Bax, and Bcl-2 immunopositive cardiomyocytes were estimated as area percent (%) across ten different fields/sections [64].…”

Section: Histomorphometric Assessmentmentioning

confidence: 99%

Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways

Elblehi

El‐Sayed

Soliman

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

Doxorubicin (DOX) has a potent antineoplastic efficacy and is considered a cornerstone of chemotherapy. However, it causes several dose-dependent cardiotoxic results, which has substantially restricted its clinical application. This study was intended to explore the potential ameliorative effect of date palm pollen ethanolic extract (DPPE) against DOX-induced cardiotoxicity and the mechanisms underlying it. Forty male Wistar albino rats were equally allocated into Control (CTR), DPPE (500 mg/kg bw for 4 weeks), DOX (2.5 mg/kg bw, intraperitoneally six times over 2 weeks), and DPPE + DOX-treated groups. Pre-coadministration of DPPE with DOX partially ameliorated DOX-induced cardiotoxicity as DPPE improved DOX-induced body and heart weight changes and mitigated the elevated cardiac injury markers activities of serum aminotransferases, lactate dehydrogenase, creatine kinase, and creatine kinase-cardiac type isoenzyme. Additionally, the concentration of serum cardiac troponin I (cTnI), troponin T (cTnT), N-terminal pro-brain natriuretic peptide (NT-pro BNP), and cytosolic calcium (Ca+2) were amplified. DPPE also alleviated nitrosative status (nitric oxide) in DOX-treated animals, lipid peroxidation and antioxidant molecules as glutathione content, and glutathione peroxidase, catalase, and superoxide dismutase activities and inflammatory markers levels; NF-κB p65, TNF-α, IL-1β, and IL-6. As well, it ameliorated the severity of histopathological lesions , histomorphometric alteration and improved the immune-staining of the pro-fibrotic (TGF-β1), pro-apoptotic (caspase-3 and Bax), and anti-apoptotic (Bcl-2) proteins in cardiac tissues. Collectively, pre-coadministration of DPPE partially mitigated DOX-induced cardiac injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and anti-apoptotic potential.

show abstract

“…In acrylamide intoxication, an increase in dopamine expression has also been shown in the rat striatum [34]. Furthermore, acrylamide results in a reduction of enzymatic and non-enzymatic antioxidants and in lipid peroxidation, which leads to nerve cell apoptosis, and the latter phenomenon is involved in the pathogenesis of many neurodegenerative diseases [35,36]. This has also been demonstrated in some studies on acrylamide-induced reactive gliosis, presenting with a potentiated synthesis of free radicals and stimulating amino acids and proinflammatory cytokines in the glial cells, which results in neuronal death [35].…”

Section: Discussionmentioning

confidence: 99%

Effect of Acrylamide Supplementation on the CART-, VAChT-, and nNOS-Immunoreactive Nervous Structures in the Porcine Stomach

Palus

Bulc

Całka

2020

Animals

View full text Add to dashboard Cite

Acrylamide is found in food products manufactured with high-temperature processing, and exposure to acrylamide contained in food products may cause a potential risk to human health. The aim of this investigation was to demonstrate the changes in the population of CART-, nNOS-, and VAChT-immunoreactive enteric neurons in the porcine stomach in response to supplementation of low and high acrylamide doses. The study was carried out with 15 Danish landrace gilts divided into three experimental groups: the control group—animals were administered empty gelatine capsules; the low-dose group—animals were administrated a tolerable daily intake (TDI) dose (0.5 µg/kg of body weight (b.w.)/day) of acrylamide capsules, and the high-dose group—animals were administrated high-dose (ten times higher than TDI: 5 µg/kg b.w./day) acrylamide capsules for 28 days. Using the double immunofluorescence staining method, it was established that supplementation with low and high doses of acrylamide resulted in alterations of the porcine stomach neuron phenotype, which was reflected in an increased number of CART-, VAChT-, and nNOS-immunoreactive neurons. These changes were accompanied by an increased density of CART-, VAChT-, and nNOS-positive fibres. The results suggest that the enteric nervous system plays an important role in protecting the gastrointestinal tract during acrylamide intoxication.

show abstract

Apoptosis and astrogliosis perturbations and expression of regulatory inflammatory factors and neurotransmitters in acrylamide-induced neurotoxicity under ω3 fatty acids protection in rats

Cited by 39 publications

References 79 publications

Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol

Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol

Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways

Effect of Acrylamide Supplementation on the CART-, VAChT-, and nNOS-Immunoreactive Nervous Structures in the Porcine Stomach

Contact Info

Product

Resources

About