Apoptosis and Mitotic Arrest Are Two Independent Effects of the Protein Phosphatases Inhibitor Okadaic Acid in K562 Leukemia Cells

Lerga, Ana; Richard, Carlos; Delgado, M. Dolores; Cañelles, Matilde; Frade, P; Cuadrado, M A; León, Javier

doi:10.1006/bbrc.1999.0852

Cited by 41 publications

(37 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The addition of cyclodextrins to the culture medium improved cell biomass and taxol recovery [17]. Further details are available in US Patents [16,17,18,20] In diploid parthenogenesis, 'proembryos' developed from binucleate egg-equivalents containing an egg nucleus and an apoptotic ventral canal nucleus. As the axial tier of 'early embryos' formed, the cell regulatory proteins in proembryonal cells were ubiquitinated and turned over as suspensors differentiated.…”

Section: Bmc Plant Biology 2005 5(suppl 1)mentioning

confidence: 99%

“…Thus, literature indicates that phosphorylation and dephosphorylation represent a part of the molecular mechanism responsible for both the organization of the cortical microtubular networks and of mitotic function. Studies on animal cells clearly demonstrated that okadaic acid and other protein phosphatase inhibitors induce mitotic arrest [15,16], premature chromosome condensation [17,18], microtubule disassembly [18,19], DNA fragmentation [20,21] and apoptosis [16,17,20,21]. Summarizing our data obtained we can conclude that the changes in the spatial organisation of microtubules after treatment by cAMP and the protein kinase inhibitors lead to disturbances of cell cycle progression and it is most likely to launch of the cell death program in plant cells.mitosis, the process of chromosomal division to form new cells.…”

Section: +mentioning

confidence: 99%

“…The bound taxol and taxanes at the cell-surface were deposited in newly formed cell walls or released into the culture medium. The drug-productive cells and materials in the culture medium containing bound taxol, baccatin III, and taxanes were collected with antibody-labeled paramagnetic beads [16]. Immunogold-labeled taxol, taxanes and baccatin III were detected in transport vesicles, plasma membrane fragments, and on materials recovered from the culture medium.…”

mentioning

confidence: 99%

See 2 more Smart Citations

High-scale analysis of pathogenicity determinants of Potato mop-top virus

et al. 2005

View full text Add to dashboard Cite

BMC Plant Biology 2005, 5(Suppl 1):S1The influence of UV-radiation on the 14 CO 2 assimilation rate of three high mountainous plants (Heracleum lehmannianum Bunge, Prangos pabularia Lind L. and Lathyrus mulkak Lipsky) with different photosynthetic intensity and directivity of photosynthetic carbon metabolism was studied. The investigation was carried out in the Biological Station of The Institute of Plant Physiology and Genetics (Academy of Sciences of Republic of Tajikistan), located on an austral decline (2350 m above sea level) of the Hissar valley (Tajikistan). It is proved, that plants show different responses to dissecting away of UV-rays. Almost tenfold fall in the intensity of 14 CO 2 fixation (during 30 sec) is noted for H. lehmannianum; in P. pabularia there was twofold fall in depression, and in L. mulkak the dissecting away of UV-rays resulted in the minor rising of the photosynthetic rate. Under both film dropping UV-rays and in open area, we have not revealed essential differences in the 14 CO 2 assimilation rate in all three plants. The experiments were carried out in three variants: 1. Control -open place. 2. The plants were covered with polyethylene film. 3. The plants were covered with polyethylene film enriched with 2-oxi-4-alcoxibenzophenol (0.65%), cutting off the UV part of solar spectrum. The study of 14 C incorporation into the products of photosynthesis has manifested essential differences between investigated plants. From the control experiment, using the Heracleum leaves, more than 65% of a label was included into the intermediates of the Calvin cycle (among them about 20% into PGA). The dissecting away of UV-radiation resulted in a depression of 14 C incorporation into PGA and PES. The key products, which, at 30 sec exposure, concentrated the most part of label were sugars, predominantly sucrose. Intermediates of the glycolic pathway concentrated a small part of 14 C (22%). The depressing of CO 2 photosynthetic assimilation in Prangos was accompanied by a sharp slump of 14 C incorporation into intermediates of the Calvin cycle. The label was found out in sugars predominantly and in intermediates of the glycolic pathway. In control plants we detected an increase of 14 C lobe in monosaccharides. The content of label in intermediates of glycolic pathway decreased. In Lathyrus leaves under investigation we revealed intensive label incorporation into intermediates of the Calvin cycle and its decrease in the metabolites of the glycolic pathway. We found minor quantities of 14 C contents in sugars and PEP-products. It is supposed that the UV-rays influence the activity of RUBISCO and other enzymes of the Calvin cycle. The mechanisms of UV-rays influence on carboxylation system and the possibility of their regulatory role in high mountainous plants are under discussion. S2Changes in a pattern of HMW-DNA fragmentation accompany differentiation and ageing of plant cells Nuclear DNA is arranged into loop domains by periodical attachment of chromatin fibers to nuclear matrix at highest level of c...

show abstract

Section: Bmc Plant Biology 2005 5(suppl 1)mentioning

confidence: 99%

Section: +mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

High-scale analysis of pathogenicity determinants of Potato mop-top virus

et al. 2005

View full text Add to dashboard Cite

show abstract

“…DNA Fragmentation-DNA fragmentation was basically performed as described previously (42). Briefly, to determine the presence of internucleosomal DNA fragmentation, cells were lysed in 10 mM Tris, 1 mM EDTA, and 0.2% Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

Subcellular Localization Determines the Protective Effects of Activated ERK2 against Distinct Apoptogenic Stimuli in Myeloid Leukemia Cells

Ajenjo

Cañón

Sánchez‐Pérez³

et al. 2004

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

ERKs, mitogen-activated protein kinases, are well characterized as key mediators in the conveyance of signals that promote cell survival in cells of hemopoietic origin, a key factor in the upbringing of leukemogenesis. It is also well known that ERKs phosphorylate a wide array of substrates distributed throughout distinct cellular locations such as the nucleus, cytoplasm, and cell periphery, but the relative contribution of these compartmentalized signal components to the overall survival signal generated by activation of ERKs has yet to be established. To this end, we have utilized constitutively activated forms of ERK2, whose expression is restricted to the nucleus or to the cytoplasm, to investigate the consequences of compartmentalized activation of ERK in the survival of chronic myelogenous leukemia cells subjected to distinct apoptogenic stimuli. We show that cytoplasmic ERK2 activity protected against apoptosis caused by prolonged serum starvation, whereas ERK2 activation restricted to the nucleus antagonized apoptosis induced by the Bcr-Abl inhibitor STI571. On the other hand, neither cytoplasmic nor nuclear ERK2 activities were effective in counteracting apoptosis induced by UV light. These results demonstrate that the protective effects of ERK2 against defined apoptogenic stimuli are strictly dependent on the cellular localization where ERK activation takes place. Furthermore, we present evidence suggesting that the complex I B-NF B participates on ERK2-mediated survival mechanisms, in a fashion dependent on the cellular location where ERK2 is active and on the causative apoptogenic stimulus.

show abstract

“…This toxin is also a tumor promoter (Fujiki and Suganuma, 1999), and an inducer of lipid peroxidation and apoptosis in cultured mammalian cells (Guzman and Castro, 1991;Boe et al, 1991;Matias et al, 1999a;Traoré et al, 1999Traoré et al, , 2000Lerga et al, 1999;Berven et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Combined cytotoxicity and genotoxicity of a marine toxin and seafood contaminant metal ions (chromium and cadmium)

Souid-Mensi

Moukha

Maaroufi

et al. 2008

Environmental Toxicology

View full text Add to dashboard Cite

Algal bloom with consequent production of marine toxins contaminating bivalves is increasing in costal regions worldwide because of sea water quality worsening. Contamination of seafood by diarrheic shellfish poisoning toxins (DSP) together with metals is frequently reported, a phenomenon not fully explained yet. In this context, metal ions were assayed in clams collected from the banned area of Boughrara, Tunisia, contaminated by Gymnodinium and other algae such as Dinophysis sp, accumulated by these bivalves. The presence of toxic metals ions such as Chromium (Cr) and Cadmium (Cd) in meat, shells, and water released by the clams prompted us to experiment in Caco-2 intestinal cell line toxic effects of these heavy metals ions in combination with okadaic acid, one DSP present in clams to assess the potential global toxicity. Cr and Cd produce additive effects in (i) reactive oxygen species production, (ii) cytotoxicity as assessed by the mitochondrial activity testing method (MTT test), and (iii) DNA lesions evaluated by agarose gel electrophoresis and acridine orange staining. Exaggerated DNA fragmentation is observed, suggesting an overloading of repair capacity of Caco-2 cells. The apoptosis suggested by a DNA fragment sizing (180-200 bp) in agarose gel and mechanisms underlying these additive effects in Caco-2 cells still need to be more comprehensively explained.

show abstract

Apoptosis and Mitotic Arrest Are Two Independent Effects of the Protein Phosphatases Inhibitor Okadaic Acid in K562 Leukemia Cells

Cited by 41 publications

References 40 publications

High-scale analysis of pathogenicity determinants of Potato mop-top virus

High-scale analysis of pathogenicity determinants of Potato mop-top virus

Subcellular Localization Determines the Protective Effects of Activated ERK2 against Distinct Apoptogenic Stimuli in Myeloid Leukemia Cells

Combined cytotoxicity and genotoxicity of a marine toxin and seafood contaminant metal ions (chromium and cadmium)

Contact Info

Product

Resources

About