Apoptosis and reduced influenza A virus specific CD8+ T cells in aging mice

Zhang, Yingao; Wang, Y; Gilmore, Xyanthine; Xu, Ke; Chen, M; Tebebi, P; Mbawuike, Innocent N.

doi:10.1038/sj.cdd.4401011

Cited by 22 publications

(30 citation statements)

References 93 publications

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“…Although not all studies in aged mice demonstrate a decreased susceptibility to apoptosis (18,19), there is considerable data that support our current results indicating that reduced susceptibility to apoptosis contributes to decreased response of T cells of aged mice to virus infection.…”

Section: Discussionsupporting

confidence: 78%

Cutting Edge: T Cells from Aged Mice Are Resistant to Depletion Early During Virus Infection

Jiang

Anaraki

Blank³

et al. 2003

The Journal of Immunology

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Aging is associated with decreased expansion of T cells upon stimulation. In young mice, infection induces a transient T cell depletion followed by the development of an Ag-specific T cell response that controls the infection. We found that T cells were depleted early after infection with E55 + murine leukemia retrovirus in young, but not aged, mice. Adoptive transfer experiments showed donor T cells of young, but not aged, mice were depleted due to apoptosis in various tissues of young recipients. However, T cells of neither young nor aged donors were depleted in aged recipients. These results indicate that both environmental and intrinsic cellular properties limit depletion of T cells of aged mice and suggest a novel explanation for the decreased T cell response associated with aging.

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Section: Discussionsupporting

confidence: 78%

Cutting Edge: T Cells from Aged Mice Are Resistant to Depletion Early During Virus Infection

Jiang

Anaraki

Blank³

et al. 2003

The Journal of Immunology

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“…Annexin V binding assay is a very sensitive method for evaluating the very early stages of Apoptosis. Using the method described in our previous study [21], we examined the Annexin V- positive cells. Unlike the dead cell counting and floating cell counting, the kerotinocytes (22.4±3.7%, n = 6) had significantly higher Annexin V positive percentages compared to fibroblasts (17.3±2.9%, n = 6, P<0.05) on day 15 following the culture.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Keratinocyte Proliferation and Migration in Co-culture with Fibroblasts

Wang

Farhangfar³

et al. 2012

PLoS ONE

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Wound healing is primarily controlled by the proliferation and migration of keratinocytes and fibroblasts as well as the complex interactions between these two cell types. To investigate the interactions between keratinocytes and fibroblasts and the effects of direct cell-to-cell contact on the proliferation and migration of keratinocytes, keratinocytes and fibroblasts were stained with different fluorescence dyes and co-cultured with or without transwells. During the early stage (first 5 days) of the culture, the keratinocytes in contact with fibroblasts proliferated significantly faster than those not in contact with fibroblasts, but in the late stage (11th to 15th day), keratinocyte growth slowed down in all cultures unless EGF was added. In addition, keratinocyte migration was enhanced in co-cultures with fibroblasts in direct contact, but not in the transwells. Furthermore, the effects of the fibroblasts on keratinocyte migration and growth at early culture stage correlated with heparin-binding EGF-like growth factor (HB-EGF), IL-1α and TGF-β1 levels in the cultures where the cells were grown in direct contact. These effects were inhibited by anti-HB-EGF, anti-IL-1α and anti-TGF-β1 antibodies and anti-HB-EGF showed the greatest inhibition. Co-culture of keratinocytes and IL-1α and TGF-β1 siRNA-transfected fibroblasts exhibited a significant reduction in HB-EGF production and keratinocyte proliferation. These results suggest that contact with fibroblasts stimulates the migration and proliferation of keratinocytes during wound healing, and that HB-EGF plays a central role in this process and can be up-regulated by IL-1α and TGF-β1, which also regulate keratinocyte proliferation differently during the early and late stage.

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“…This is consistent with a report that lymphopenia develops more often in older patients (14). It also suggests that, since cytokine secretion varies according to age in influenza virus-infected patients, there is a difference in the extent of cell death with patient age, and that in children over 2 yr, with mature immune systems, differences in individual immune reactions may influence the occurrence of lymphopenia, and in turn the severity of disease (25, 26). …”

Section: Discussionmentioning

confidence: 99%

Characteristics of Hospitalized Children with 2009 Pandemic Influenza A (H1N1): A Multicenter Study in Korea

Kim²,

Kang

et al. 2012

J Korean Med Sci

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The majority of Korean patients with pandemic influenza A (H1N1) during the 2009 epidemic were under 20 yr of age. The limited data on the clinical characteristics of these children led us to conduct a case note-based investigation of children admitted to 6 university hospitals with 2009 H1N1 influenza. A total of 804 children was enrolled. The median age was 5 yr; 63.8% were males; and 22.4% had at least one chronic underlying disease. Ninety-five of the patients (11.8%) were critically ill and they suffered more from shortness of breath, dyspnea and lymphopenia than the other patients. Among all the patients, 98.8% were treated with antivirals and 73% received treatment within 48 hr of illness onset. All the enrolled patients are alive and appear to have had good outcomes, probably due to the early intervention and antiviral treatment. This study deals with hospitalized children whose diagnoses of influenza A (H1N1) were confirmed, and therefore provides important new information about the clinical patterns of children with influenza A (H1N1) in Korea.

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Apoptosis and reduced influenza A virus specific CD8+ T cells in aging mice

Cited by 22 publications

References 93 publications

Cutting Edge: T Cells from Aged Mice Are Resistant to Depletion Early During Virus Infection

Cutting Edge: T Cells from Aged Mice Are Resistant to Depletion Early During Virus Infection

Enhanced Keratinocyte Proliferation and Migration in Co-culture with Fibroblasts

Characteristics of Hospitalized Children with 2009 Pandemic Influenza A (H1N1): A Multicenter Study in Korea

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