Apoptosis-Associated Gene Expression in the Corpus Luteum of the Rat1

Guo, Ke; Wolf, Vladimir; Dharmarajan, Arun; Feng, Zhiwei; Bielke, Wolfgang; Saurer, Susanne; Friis, Robert R.

doi:10.1095/biolreprod58.3.739

Cited by 78 publications

(61 citation statements)

References 28 publications

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“…We have previously shown that DDC-4/sFRP-4 was upregulated in various tissues undergoing physiological apoptosis. 3,4,45 The new data show that DDC-4/sFRP-4 expression is a stimulus rather than a consequence of the apoptotic program in the mammary gland. Since DDC-4 is an sFRP and thus thought to sequester Wnt ligands, we propose a role for DDC-4/sFRP-4 in abrogating a Wnt-regulated survival pathway, the latter possibly signaling via PKB/Akt.…”

Section: Discussionmentioning

confidence: 82%

“…45,53 The rabbit affinity purified anti-DDC-4 serum was used at a dilution of 1:100 on tissue sections after microwave treatment in 50 mM sodium citrate for 5 min at reduced energy (maximum temperature, 901C). For BrdU labeling, females were injected I.P.…”

Section: Isolation Of Rna and Northern Blottingmentioning

confidence: 99%

“…The TUNEL reaction was performed as previously described 45 using either BODIPY-dUTP visualized directly in the fluorescence microscope (Molecular Probes, Eugene, OR, USA) or digoxigenin-11 dUTP detected with anti-digoxigenin Fab antibodies conjugated to alkaline phosphatase (Roche Diagnostics, Rotkreuz, Switzerland). Fixation was exactly as for in situ hybridization.…”

Section: Terminal Deoxynucleotidyl Transferase-mediated Dutp Nick Endmentioning

confidence: 99%

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Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

et al. 2003

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Using differential display, we isolated DDC-4, a secreted frizzled-related protein (sFRP), which is induced in the physiological apoptosis of hormonally regulated, reproductive tissues such as mammary gland, prostate, corpus luteum and uterus. The role of this gene in apoptosis was studied in animals overexpressing ectopic DDC-4/sFRP-4. Transgenic mice bearing the DDC-4/sFRP-4 cDNA under the control of the MMTV-LTR promoter showed lactational insufficiency and many apoptotic cells in the alveoli between day 19 of pregnancy and day 4 of lactation as demonstrated by TUNEL reaction and the presence of activated caspase-3. We performed a PKB/Akt kinase assay and studied several of its substrates using phosphorylation-specific antibodies to show reduced phosphorylation in PKB/Akt itself, as well as in glycogen synthetase kinase-3b (GSK-3b), BAD, and Forkhead. Taken together, our results show a role for DDC-4/sFRP-4 in abrogating an epithelial cell survival pathway at the onset of mammary gland involution.

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Section: Discussionmentioning

confidence: 82%

Section: Isolation Of Rna and Northern Blottingmentioning

confidence: 99%

Section: Terminal Deoxynucleotidyl Transferase-mediated Dutp Nick Endmentioning

confidence: 99%

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Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

et al. 2003

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“…At the end of pregnancy in the rat, the most evident sign of luteal regression is the abrupt decrease in P 4 secretion by the luteal cells, which is followed by their apoptotic death (Guo et al 1998). The factors responsible for initiating and mediating luteal regression are very complex.…”

Section: Introductionmentioning

confidence: 99%

Hypothyroidism prolongs corpus luteum function in the pregnant rat

Hapon¹,

Motta²,

Ezquer³

et al. 2007

Reproduction

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It has been shown that hypothyroidism in the rat produces a prolongation of pregnancy associated with a delay in the fall of circulating progesterone (P 4 ) at term. The aim of the present work is to determine whether the delayed P 4 decline in hypothyroid mother rats is due to a retarded induction of P 4 degradation to 20aOH P 4 or to a stimulation of its synthesis, and to investigate the possible mechanisms that may underlie the altered luteal function. We determined by RIA the circulating profile of the hormones (TSH, PRL, LH, P 4 , PGF2a, and PGE2) involved in luteal regulation at the end of pregnancy and, by semiquantitative RT-PCR, the expression of factors involved in P 4 synthesis (CytP450scc, StAR, 3bHSD, PRLR) and metabolism (20aHSD, PGF2aR, iNOS and COX2). Our results show that the delay in P 4 decline and parturition is the resultant of retarded luteal regression, caused by a combination of decreases in luteolytic factors, mainly luteal PGF2a, iNOS mRNA expression and also circulating LH, and increased synthesis or action of luteotrophic factors, such as luteal and circulating PGE2 and circulating PRL. All these changes may be direct causes of the decreased 20aHSD mRNA and protein (measured by western blot analysis) expression, which in the presence of unchanged expression of the factors involved in P 4 synthesis results in elevated luteal and circulating P 4 that prolonged pregnancy and also may favor longer survival of the corpus luteum.

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“…The ro le of sFRP downre gu la ti on in bre ast can cers has been furt her con fir med by a number of aut hors. [30][31][32][33] In this study, we in ves ti ga ted; 1-Ex pres si on le vels of β-ca te nin, cyclin D1, sFRP1 and sFRP2 in the bre ast can cer tis su e, 2-The inf lu en ce of me no pa u sal sta tus, es tro gen re cep tor, pro ges te rone re cep tor, c-erbB2, p53 po si ti vity, lymph no de in vol ve ment and cli ni cal TNM sta ge on the expres si on of β-ca te nin, cyclin D1, sFRP1 and sFRP2, 3-The ef fect of the eva lu a ted pa ra me ters on β-ca te nin, cli ni copat ho lo gi cal prog nos tic pa rame ters inc lu ding his to lo gi cal gra de, lymph no de in vol ve ment and TNM sta ge.…”

mentioning

confidence: 99%

β-Catenin Stability, Cyclin D1 and Frizzled Proteins Expression in Human Breast Cancer and Their Relation with the Prognosis

Zengel¹,

Vardar²,

Postacı³

et al. 2011

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Aberrant Wnt signaling pathway activation and stabilization of β-catenin protein is associated with tumorigenesis and human breast tumors. We have examined β-catenin, cyclin D1, sFRP1 and sFRP2 expressions in the breast cancer and effects of these factors on prognostic parameters in patients with breast cancer. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : One hundred and seventeen consecutive female malignant breast tumor patients were enrolled into the study. The mean age was 50.0 (±18.0) years. Immunohistochemical staining method was used to investigate the stability and location of β-catenin, expressions of sFRP1 and sFRP2 and cyclin D1 proteins. R Re es su ul lt ts s: : Percentage expression of β-catenin, siklin D1, sFRP1 and sFRP2 were 60.0 ± 55.0, 40.0 ± 64.0, 15.0±48.0 and 25.0 ± 53.0, respectively. Menopausal status, progesterone receptor positivity, lymph node involvement and TNM staging did not show any statistically significant relation with the expression of β-catenin, cyclin D1, sFRP1 or sFRP2. However, significantly higher percentages of expression of cyclin D1 were determined in patients showing estrogen receptor positivity and cerbB-2 over-expression (p= 0.008). Additionally β-catenin expression was significantly higher only in the p53-positive group (p= 0.03). None of the parameters used in this study showed a significant effect on clinicopathological prognostic parameters. C Co on nc cl lu us si io on n: : These findings suggested that expression levels and staining results of β-catenin, cyclinD1, sFRP1 and sFRP2 in tumor cells were independent from histological grade, lymph node involvement, and TNM stage. We concluded that β-catenin accumulation, cyclin D1, sFRP1 and sFRP2 expressions were not affected by the Wnt signaling pathway. K Ke ey y W Wo or rd ds s: : Breast neoplasm; β-catenin; cyclin D1; secreted frizzled related proteins Ö ÖZ ZE ET T A Am ma aç ç: : Wnt sinyal yolağının aberan aktivasyonu ve β-katenin proteininin stabilizasyonu tümör gelişimi ve insan meme tümörleri ile ilişkilidir. Bu çalışmada, meme kanserinde β-katenin, siklin D1, sFRP1 ve sFRP2 ekspresyonlarını ve bu faktörlerin hastaların prognostik parametreleri üzerine etkilerini incelemeyi amaçladık. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Ortalama yaşı 50 ± 18 olan ardışık 117 meme kanserli kadın olgu çalışmaya alındı. İmmünositokimyasal yöntemle β-katenin stabilitesi, sFRP1, sFRP2 ve siklin D1 proteinlerinin ekspresyonları değerlendirildi. B Bu ul lg gu ul la ar r: : β-katenin, siklin D1, sFRP1 ve sFRP2'nin ekspresyonları sırasıyla %60.0 (±55.0), %40.0 (±64.0), %15.0 (±48.0) ve %25.0 (±53.0) idi. Menopozal durum, progesteron reseptörü, lenf nodu tutulumu, TNM evresi ile β-katenin, siklin D1, sFRP1 veya sFRP2 ekspresyonu arasında istatistiksel olarak anlamlı bir ilişki bulunmadı. Bununla birlikte olgularda östrojen pozitifliği ve cerbB-2 pozitifliği ile siklin D1 arasında istatistiksel olarak anlamlı ilişki varlığı izlendi (p= 0.008). β-...

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Apoptosis-Associated Gene Expression in the Corpus Luteum of the Rat1

Cited by 78 publications

References 28 publications

Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

Hypothyroidism prolongs corpus luteum function in the pregnant rat

β-Catenin Stability, Cyclin D1 and Frizzled Proteins Expression in Human Breast Cancer and Their Relation with the Prognosis

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