Apoptosis by direct electric field (DEF) and nanosecond pulsed electric field (nsPEF) in tumor cells and tumor tissues

Kim, H.B.; Ahn, Seong-Hun; Sim, Sung Bo

doi:10.1109/plasma.2003.1230010

Cited by 1 publication

(1 citation statement)

References 2 publications

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“…Despite an interesting approach, it is worth mentioning that several studies link the DNA damage caused by nsPEF to cell apoptosis. In fact, DNA damage can be described as a secondary effect of nsPEF application [ 3 , 19 , 69 ]. Notably, Chen and co-workers showed a strong nsPEF effect on the HL-60 cell nucleus [ 70 ].…”

Section: Nspef-induced Damage In Cell’s Dnamentioning

confidence: 99%

Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?

2022

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Since the beginning of 2020, worldwide attention has been being focussed on SARS-CoV-2, the second strain of the severe acute respiratory syndrome virus. Although advances in vaccine technology have been made, particularly considering the advent of mRNA vaccines, up to date, no single antigen design can ensure optimal immune response. Therefore, new technologies must be tested as to their ability to further improve vaccines. Nanosecond Pulsed Electric Field (nsPEF) is one such method showing great promise in different biomedical and industrial fields, including the fight against COVID-19. Of note, available research shows that nsPEF directly damages the cell’s DNA, so it is critical to determine if this technology could be able to fragment either viral DNA or RNA so as to be used as a novel technology to produce inactivated pathogenic agents that may, in turn, be used for the production of vaccines. Considering the available evidence, we propose that nsPEF may be used to produce inactivated SARS-CoV-2 viruses that may in turn be used to produce novel vaccines, as another tool to address 20 the current COVID-19 pandemic. Key Messages Viral inactivation by using pulsed electric fields in the nanosecond frequency. DNA fragmentation by a Nanosecond Pulsed Electric Field (nsPEF). Opportunity to apply new technologies in vaccine development.

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