Apoptosis Evaluation by Electrochemical Techniques

Yin, Jian

doi:10.1002/asia.201501045

Cited by 16 publications

(6 citation statements)

References 84 publications

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“…Caspase-3 is an activated cysteine protease that plays an important role in intrinsic and extrinsic apoptotic pathways. Therefore, caspase-3 has been recognized as a reliable molecular biomarker and therapeutic target for apoptosis-related diseases . To demonstrate the application of the biosensor for apoptosis evaluation, caspase-3 in living and apoptotic MCF-7 cells was analyzed.…”

Section: Resultsmentioning

confidence: 99%

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

et al. 2021

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This work proposed a new sensing strategy for protease detection by converting a homogeneous assay into a surface-tethered electrochemical analysis. Streptavidin (SA), a tetramer protein, was used as the sensing unit based on the SA–biotin coupling chemistry. Caspase-3 was used as the model analyte, and a biotinylated peptide with a sequence of biotin–GDEVDGK–biotin was designed as the substrate. Specifically, the peptide substrate could induce an assembly of SA to form (SA–biotin–GDEVDGK–biotin) n aggregates through SA–biotin interactions, which was confirmed by atomic force microscopy (AFM). The peptide substrate-induced assembly of SA was facilely initiated on an electrode–liquid surface by modification of the electrode with SA. The in situ formation of (SA–biotin–GDEVDGK–biotin) n aggregates created an insulating layer, thus limiting the electron transfer of ferricyanide. Once the peptide substrate was cleaved into two shorter fragments (biotin–GDEVD and GK–biotin) by caspase-3, the resulting products would compete with biotin–GDEVDGK–biotin to bind SA proteins immobilized on the electrode surface and distributed in a solution, thus preventing the in situ formation of (SA–biotin–GDEVDGK–biotin) n assemblies. With the simple principle of the substrate-induced assembly of SA, a dual-signal amplification was achieved with improved sensitivity. Taking advantage of high sensitivity, simple principle, and easy operation, this method can be augmented to design various surface-tethered biosensors for practical applications.

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Section: Resultsmentioning

confidence: 99%

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

et al. 2021

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“…In the present study, apoptosis was assessed by using Annexin V, which is a protein that binds to the cell surface of apoptotic cells (28). Apoptosis is the process of programmed cell death that occurs in multicellular organisms (29,30). It has previously been demonstrated that curcumin may be used in the treatment of cancer due to its ability to induce apoptosis and inhibit angiogenesis (31,32).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of curcumin in human endometriosis endometrial cells via downregulation of vascular endothelial growth factor

Cao

Wei

Zhou

et al. 2017

Molecular Medicine Reports

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Endometriosis, which affects up to 10% of women of reproductive age, is defined as endometrial-like gland and stroma tissue growths outside the uterine cavity. Despite increasing research efforts, there are no current effective treatment methods for this disease, therefore investigations for therapeutic strategies are of primary concern. In preliminary work, the authors demonstrated that curcumin inhibits endometriosis in vivo. The present in vitro study aimed to investigate the association between endometriotic stromal cells and curcumin and to clarify the underlying mechanism of action. A total of 14 patients with endometriosis were enrolled in the present study. The purity of endometrial stromal cell cultures was proven by standard immunofluorescent staining of vimentin. The cell proliferation and curcumin effects on endometrial stromal cells were assessed by the MTT assay and Hematoxylin and Eosin staining. For cell cycle analysis, phase distribution was detected by flow cytometry. Vascular endothelial growth factor (VEGF) protein expression was examined using immunohistochemistry staining. Apoptosis was assessed using Annexin V‑fluorescein isothiocyanate staining. The results indicated that the treatment of curcumin decreased human ectopic and eutopic stromal cell growth. Following treatment with curcumin, human endometriotic stromal cells demonstrated an increased percentage of G1‑phase cells and decreased percentages of S‑phase cells, particularly in the group treated with 50 µmol/l curcumin. Treatment with curcumin additionally decreased expression of VEGF. The data provide evidence that curcumin reduces cell survival in human endometriotic stromal cells, and this may be mediated via downregulation of the VEGF signaling pathway.

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“…The presence of mutations in circulating tumor DNA (ctDNA) as well as in RNA or microRNA (miRNA) and their amount can serve as diagnostic biomarkers for various oncologic diseases, predictive markers for analyzing the response to medical treatment, and/or as markers of disease progression [1]. Thereby, alteration of DNA length (DNA fragmentation) is one of the accepted markers of programmed cell death (apoptosis) [2][3][4]. In that regard, the analysis of modified heterocyclic bases (e.g.…”

Section: Introductionmentioning

confidence: 99%

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Sigolaeva

Булко

Kozin

et al. 2019

Polymer

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This work demonstrates the use of imidazolium-based poly(ionic liquid)s (PILs) as efficient dispersants of multi-walled carbon nanotubes (MWCNTs). With these polymeric dispersants, highly stable fine dispersions of MWCNTs (inks) can be easily prepared in aqueous media and applied for rather simple but efficient surface modification of screen-printed electrodes (SPEs). Such a modification of SPEs remarkably increases the electroactive surface area and accelerates the electron transfer rate due to synergistic combination of specific features of MWCNTs such as strong adsorptive property and high specific surface with the advantages of PILs like ion conductivity and dispersability. We further show that the PIL/MWCNT-modified SPEs can be beneficially utilized for direct electrochemical analysis of double stranded DNA (dsDNA). Specifically, it is exemplified by the direct electrooxidation of guanine and adenine bases in salmon testes dsDNA chosen as a model system. The linear ranges for the determination of dsDNA correspond to 5-500 µg/mL for the oxidative peak of guanine and 0.5-50 µg/mL for the oxidative peak of adenine. This makes direct electrochemical dsDNA detection with the use of the easy-preparable PIL/MWCNT-modified SPEs strongly competing to currently applied spectral and fluorescent techniques. Furthermore, we show that the developed constructs are capable of sensing a single point mutation in the 12-bases single-stranded DNA fragments. Such detection is of high clinical significance in choosing an adequate anticancer treatment, where the electrochemical identification of the point mutation could offer time and cost benefits.

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Apoptosis Evaluation by Electrochemical Techniques

Cited by 16 publications

References 84 publications

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

Protease Biosensor by Conversion of a Homogeneous Assay into a Surface-Tethered Electrochemical Analysis Based on Streptavidin–Biotin Interactions

Inhibitory effect of curcumin in human endometriosis endometrial cells via downregulation of vascular endothelial growth factor

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

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