Apoptosis Factors of Lens Epithelial Cells Responsible for Cataractogenesis in Vitrectomized Eyes with Silicone Oil Tamponade

Zhu, Lili; Zhao, Ke; Lou, Dong

doi:10.12659/msm.897630

Cited by 11 publications

(14 citation statements)

References 37 publications

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“…This may explain the presence of silicone particles inside the clear crystalline lens cells detected in this study. The expression of biochemical apoptotic markers such as B-cell lymphoma 2-associated X protein (BAX) and caspase-3 increased in the epithelial cells of cataractous lenses due to silicone oil tamponade in a study by Zhu et al, 9 and this is concordant with the apoptotic changes in clear crystalline lens epithelial cells detected in this study. Although emulsified silicone was not found in our study, we detected apoptotic changes in lens epithelial cells.…”

Section: Discussionsupporting

confidence: 89%

“…This was also confirmed in a study in which there was no correlation between silicone emulsification and increased apoptotic marker expression in patients with complicated cataract due to silicone oil tampnade. 9 While previous studies have investigated changes in capsule and lens epithelial cells in cataractous lenses after silicone oil tamponade, 7,14 our study investigated such changes in clear lenses at the time of silicone oil removal. Miyamoto et al 14 studied ultrastructural changes in the posterior surface of the anterior capsule following lensectomy and vitrectomy with or without silicone oil tamponade in rabbits and reported the presence of many vacuoles on the capsular surface, suggesting the deposition of emulsified silicone oil droplets.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have studied the ultrastructural effects of silicone oil tamponade on the anterior lens capsule and its epithelium in cataractous lenses. [5][6][7][8][9] The aims of this study were to investigate the clinically unseen cataractogenic effects of silicone oil via light and electron microscopy of clear lens anterior capsules and their epithelium in highly myopic vitrectomized eyes with silicone oil tamponade and to compare those capsules with anterior lens capsules obtained by refractive clear lens extraction (RLE) in highly myopic eyes.…”

Section: Introductionmentioning

confidence: 99%

“…Researchers have studied the ultrastructural effects of silicone oil tamponade on the anterior lens capsule and its epithelium in cataractous lenses. 5–9…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural effects of silicone oil on the clear crystalline lens of the human eye

Soliman

Saad

Abdelazeem

et al. 2018

European Journal of Ophthalmology

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silicone oil may play a role in the development of apoptotic and histopathological changes in clear lens epithelial cells. Clarity of the lens at the time of silicone oil removal does not indicate an absence of cataractous changes. We found justification of combined clear lens extraction and silicone oil removal or combined phacovitrectomy when silicone oil injection is planned, but further long-term studies with larger patient groups are required.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Researchers have studied the ultrastructural effects of silicone oil tamponade on the anterior lens capsule and its epithelium in cataractous lenses. 5–9…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrastructural effects of silicone oil on the clear crystalline lens of the human eye

Soliman

Saad

Abdelazeem

et al. 2018

European Journal of Ophthalmology

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“…The specific ratio of Bcl-2 to BAX gene expression is critical in determining cell fate [35]. Caspases are important indicators of apoptosis and caspase-3 is a major downstream effector caspase in the LEC apoptotic process [36]. Following UVB exposure, the extent of apoptosis as well as the transcription of BAX, caspase 3, FOXO3A and MT2 in HLECs were significantly increased, indicating that oxidative damage was stimulated.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antioxidant effects of different histone deacetylase inhibitors (HDACis) on human lens epithelial cells (HLECs) after UVB exposure

et al. 2019

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BackgroundTo compare the protective effects of the histone deacetylase inhibitors (HDACis) β-hydroxybutyrate (βOHB), trichostatin A (TSA), suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) on human lens epithelial cells(HLECs) following ultraviolet-B (UVB) exposure.MethodsHLECs were divided into subgroups: four HDACi groups, a control group, a UVB-treated group and a DMSO group (cells treated with DMSO and UVB irradiation). In the HDACi groups, HLECs were cultured with different concentrations of HDACis 12 h prior to UVB irradiation. The protective effects of the HDACis were evaluated by assessing apoptosis rates, cell activity and expression levels of genes associated with apotosis (caspase-3, Bcl-2, BAX, SOD1, FOXO3A and MT2). The levels of superoxide dismutase (SOD), reactive oxygen species (ROS), malondialdehyde (MDA) and total antioxidant capacity (T-AOC) were detected in order to evaluate oxidative stress.ResultsThe results showed that SAHA (1 μmol/L, 2 μmol/L) and TSA (0.2 μmol/L) had mild protective effects on cell viability. βOHB (4 mmol/L) and TSA (0.2 mol/L) demonstrated protective effects on BCL-2 expression. TSA (0.2 mol/L) showed protective effects on SOD1 expression. TSA (0.2 mol/L) and SAHA (1 μmol/L) suppressed BAX and caspase-3 expression. TSA (0.2 mol/L, 0.8 mol/L) and SAHA (1 μmol/L, 2 μmol/L) suppressed the expression of FOXO3A and MT2. SOD levels were increased after treatment with βOHB (4 mmol/L), SAHA (8 μmol/L) and TSA (0.1 mol/L, 0.2 mol/L). T-AOC levels were increased in UVB-treated HLECs after treatment with SAHA (2 μmol/L). MDA levels decreased in UVB-treated HLECs following treatment with TSA (0.2 mol/L, 0.8 mol/L). ROS levels decreased in UVB-treated HLECs following treatment with βOHB (4 mmol/L), SAHA (1 μmol/L, 2 μmol/L) and TSA (0.2 mol/L). Western blotting results demonstrated that SOD1 levels significantly increased in the βOHB (4 mmol/L), SAHA (1 μmol/L, 2 μmol/L), TSA (0.1 mol/L, 0.2 mol/L) and VPA (5 mmol/L) groups. Only SAHA (1 μmol/L) had an anti-apoptotic effect on UVB-treated HLECs.ConclusionsOur findings indicate that low concentrations of HDACis (1 μmol/L of SAHA) mildly inhibit oxidative stress, thus protecting HLECs from oxidation. These results may suggest that there is a possibility to explore the clinical applications of HDACis for treatment and prevention of cataracts.

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Fibrotic cataract as a sign of posterior capsule violation post vitrectomy

Williams¹,

Smith²,

Huvard³

et al. 2021

American Journal of Ophthalmology Case Reports

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Apoptosis Factors of Lens Epithelial Cells Responsible for Cataractogenesis in Vitrectomized Eyes with Silicone Oil Tamponade

Cited by 11 publications

References 37 publications

Ultrastructural effects of silicone oil on the clear crystalline lens of the human eye

Ultrastructural effects of silicone oil on the clear crystalline lens of the human eye

Evaluation of the antioxidant effects of different histone deacetylase inhibitors (HDACis) on human lens epithelial cells (HLECs) after UVB exposure

Fibrotic cataract as a sign of posterior capsule violation post vitrectomy

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