Apoptosis in cancer: Key molecular signaling pathways and therapy targets

Burz, Claudia; Berindan‐Neagoe, Ioana; Bălăcescu, Ovidiu; Irimie, Alexandru

doi:10.1080/02841860902974175

Cited by 200 publications

(150 citation statements)

References 71 publications

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“…6). It was reported that apoptosis can be induced through caspases-independent pathways, directly through AIF or Endo G release from mitochondria leading to apoptosis (49).…”

Section: Discussionmentioning

confidence: 99%

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Chung

Liu

et al. 2011

Int J Oncol

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Abstract. Diallyl sulfide (DAS), one of the main active constituents of garlic, causes growth inhibition of cancer cells in vitro and promotes immune responses in vivo in experimental settings. However, its effects on the induction of cell cycle and apoptosis in human cervical cancer cells are still unclear. The aims of this study were to explore the anti-cancer effects of DAS in HeLa human cervical cancer cells and to investigate the underlying mechanisms in vitro. Cytotoxicity and apoptosis in HeLa human cervical cancer cells were examined by the morphological changes, viability assay, 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining, comet assay, Western blotting and confocal microscopy examination. The results showed that DAS treatment for 24-72 h resulted in a marked decrease in cell viability time-and dose-dependently. Flow cytometric analysis showed that a 48-h treatment of 75 µM DAS induced G0/G1 cell cycle arrest and sub-G1 phase (apoptosis) in HeLa cells. Typical apoptotic nucleus alterations were observed by fluorescence microscopy in HeLa cells after exposure to DAS using DAPI staining. Cells treated with different concentrations of DAS also showed changes typical of apoptosis such as morphological changes, DNA damage and fragmentation, dysfunction of mitochondria, cytochrome c release and increased expression of pro-caspase-3 and -9. DAS also promoted the release of AIF and Endo G from mitochondria in HeLa cells. In conclusion, DAS induced G0/G1 cell cycle arrest and apoptosis in HeLa cells through caspase-and mitochondria and p53 pathways providing further understanding of the molecular mechanisms of DAS action in cervical cancer. This study, therefore, revealed that DAS significantly inhibits the growth and induces apoptosis of human cervical cancer HeLa cells in vitro.

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“…6). It was reported that apoptosis can be induced through caspases-independent pathways, directly through AIF or Endo G release from mitochondria leading to apoptosis (49).…”

Section: Discussionmentioning

confidence: 99%

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Chung

Liu

et al. 2011

Int J Oncol

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show abstract

“…IAPs and Bcl-2 (Burz, Berindan-Neagoe et al 2009). Among IAPs, cIAP-1 and cIAP-2 play key roles in the regulation of death-receptor-mediated apoptosis, whereas the X-linked IAP (XIAP) inhibits death-receptor-as well as mitochondria-mediated apoptosis by inhibiting caspase-3/-7 and caspase-9, key regulators for apoptosis (Chai, Shiozaki et al 2001).…”

Section: Escin Induces Cell Cycle Arrest At G2/m Phase and Modulates mentioning

confidence: 99%

Cytotoxic Effects of Escin on Human Castration-resistant Prostate Cancer Cells Through the Induction of Apoptosis and G2/M Cell Cycle Arrest

Piao

Kang

Lee

et al. 2014

Urology

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“…These damages lead to cell death apoptosis, when the capacities of cells for repairing damages and for detoxifying ROS are exceeded (Bellance, 2009;Gogvadze, 2009;Grüning, 2010;Israël, 2004Israël, , 2005Kroemer & Pouyssegur, 2008;Olovnikov, 2009;Vander Heiden, 2009). Cells may also develop drug resistance by over expressing anti-apoptotic proteins (Burz, 2009;Green, 2004;Yip, 2008), or by over expressing the transporter Pglycoprotein 170, a protein which expels the chemotherapy drug outside at their membrane. This membrane carrier belongs to the family of the ATP transporters associated with the Multi Drug Resistance phenotype (MDR) (Comerford, 2002).…”

Section: Discussionmentioning

confidence: 99%

Why Anti-Energetic Agents Such as Citrate or 3-Bromopyruvate Should be Tested as Anti-Cancer Agents: Experimental In Vitro and In Vivo Studies

Icard¹,

Zhang²,

Varin³

et al. 2012

Mesotheliomas - Synonyms and Definition, Epidemiology, Etiology, Pathogenesis, Cyto-Histopathological Features, Clinic, Diagnos

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Apoptosis in cancer: Key molecular signaling pathways and therapy targets

Cited by 200 publications

References 71 publications

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Cytotoxic Effects of Escin on Human Castration-resistant Prostate Cancer Cells Through the Induction of Apoptosis and G2/M Cell Cycle Arrest

Why Anti-Energetic Agents Such as Citrate or 3-Bromopyruvate Should be Tested as Anti-Cancer Agents: Experimental In Vitro and In Vivo Studies

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