Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

Yu, Yuan Song; Sui, Hong; Zheng, Han; Li, Wei; Luo, Ming; Tan, Jing

doi:10.1038/sj.cr.7290234

Cited by 145 publications

(95 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This indicates that the WID system we have developed can best mimic group culture in efficiency, while allows identification of oocyte origin. Our previous studies have shown that GCs apoptosis is an underlying mechanism of follicular atresia in the goat (Yu et al 2003(Yu et al , 2004 as in other animals (Liu et al 2003). Most of the studies on the effect of GCs apoptosis on oocyte developmental competence have so far been conducted in the bovine and using group culture systems.…”

Section: Discussionmentioning

confidence: 99%

“…It is recognized that apoptotic cell death is an underlying mechanism of cell loss during follicular atresia (Tilly et al 1991, Hsueh et al 1994, Liu et al 2003, Yu et al 2003, 2004. While the detection of granulosa cells (GCs) apoptosis has been widely used to identify atretic follicles, very little evidence for its use as a marker of oocyte quality exists.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

View full text Add to dashboard Cite

Interactive effects of granulosa cell apoptosis, follicle size, cumulus-oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system Zheng-Bin Han, Guo-Cheng Lan, Yan-Guang Wu, Dong Han, Wei-Guo Feng, Jun-Zuo Wang and Jing-He Tan AbstractUsing a well-in-drop (WID) oocyte/embryo culture system that allows identification of follicular origin, we have investigated the effects of granulosa cells (GCs) apoptosis, follicle size, cumulus-oocyte complexes (COCs) morphology, and cumulus expansion on the developmental competence of goat oocytes matured and cultured individually following parthenogenetic activation. The WID system supported oocyte maturation and embryo development to a level similar to the conventional group system. The majority of goat oocytes acquired competence for development up to the 8-16 cell stage in follicles larger than 2 mm, but did not gain the ability to form morula/blastocyst (M/Bs) until follicles larger than 3 mm in diameter. The extent of atresia affected M/Bs formation. This effect varied according to the follicle size. Cumulus expansion increased with follicle size and decreased with increasing incidence of GCs apoptosis. Oocyte developmental potential was also correlated with cumulus expansion. Regardless of the degree of follicle atresia, 73-84% of the floating cells in the follicular fluid (FF) underwent apoptosis. Correlation between floating cell density in FF and oocyte developmental potency suggests the possibility to use the floating cell density as a simple and non-invasive marker for oocyte quality. It is concluded that the developmental potential of an oocyte is determined by multifactor interactions, and multiple factors must be considered together to accurately predict the quality of an oocyte. Reproduction (2006) 132 749-758Introduction A number of studies have shown that in vitro development of pre-implantation stage mammalian embryos is better when cultured in groups (group culture) than cultured singly in microdrops (microdrop individual culture, MIC) (Gardner et al. 1994, Kato & Tsunoda 1994, Keefer et al. 1994, Moessner & Dodson 1995. However, apart from the large-scale production of embryos for experimental purposes, most commercial and human embryo culture systems require culturing individually or in small groups. The reason for this need is the small number of oocytes available (ovum pick-up (OPU) technology, single slaughtered valuable oocyte donors and human IVF) or the required identification after culture. Besides, sometimes zona-free eggs must be cultured in isolation from each other to prevent conglomeration. Therefore, the establishment of an individual culture system that is as efficient as group culture has become of prime importance. Furthermore, although there are quite a few reports on individual culture of embryos, studies on individual culture of oocytes during maturation are few and with conflicting results (Carolan et al. 1996, Hagemann et al. 1998, Jewgenow et al. 19...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

View full text Add to dashboard Cite

show abstract

“…This is possibly due to an insufficient response to nutrition by the CL to secrete progesterone or insufficient energy to influence progesterone secretion during the luteal phase of the estrous cycle. The level of progesterone was not significantly different among atretic, slightly atretic, and healthy follicles (Yu et al 2004), but testosterone elevated at all stages of follicular growth in the later stages of follicular atresia (Moor et al 1978, Carson et al 1981. The follicular fluid testosterone concentration was decreased in follicles O2.5 mm except in R group ewes (Fig.…”

Section: Parametersmentioning

confidence: 99%

Effect of different levels of short-term feed intake on folliculogenesis and follicular fluid and plasma concentrations of lactate dehydrogenase, glucose, and hormones in Hu sheep during the luteal phase

Ying¹,

Wang²,

Wang³

et al. 2011

Reproduction

View full text Add to dashboard Cite

This study investigated the effects of short-term food restriction or supplementation on folliculogenesis and plasma and intrafollicular metabolite and hormone concentrations. Ewes were randomly assigned to three groups: the control group received a maintenance diet (M) while the supplemented group and restricted group received 1.5!M and 0.5!M respectively on days 6-12 of their estrous cycle. Estrus was synchronized by intravaginal progestogen sponges for 12 days. On days 7-12, blood samples were taken. After slaughter, the ovarian follicles were classified and the follicular fluid was collected. Compared with restriction, supplementation shortened the estrous cycle length, decreased the number of follicles 2.5-3.5 mm and follicular fluid estradiol (E 2 ) concentration, increased the number of follicles O3.5 mm and plasma glucose, insulin and glucagon concentrations, and augmented the volume of follicles O2.5 mm. Restricted ewes had higher intrafollicular insulin concentration, but it was similar to that of supplemented ewes. Compared with follicles %2.5 mm, the intrafollicular glucose and E 2 concentrations were increased and the testosterone, insulin, and glucagon concentrations and lactate dehydrogenase (LDH) activity were decreased in follicles O2.5 mm. Only in restricted ewes were intrafollicular LDH and testosterone concentrations in follicles %2.5 mm not different from those in follicles %2.5 mm. In conclusion, the mechanism by which short-term dietary restriction inhibits folliculogenesis may involve responses to intrafollicular increased E 2 , testosterone, and LDH levels in late-stage follicles. This may not be due to the variation of intrafollicular insulin level but rather due to decreased circulating levels of glucose, insulin, and glucagon.

show abstract

“…Suggested mechanisms of ovarian insufficiency rely on either a decreased oocyte pool [12], augmented follicular atresia [51,112] and/or altered folliculogenesis [78]. The clinical presentation of ovarian insufficiency varies significantly.…”

Section: Fmr1 Gene Mutations and Reproductive Agingmentioning

confidence: 99%

The impact of FMR1 gene mutations on human reproduction and development: a systematic review

Noto

Harrity

Walsh

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This is a comprehensive review of the literature in this field attempting to put the FMR1 gene and its evaluation into context, both in general and for the reproductive health audience. Methods Online database search of publications with systematic review of all papers relevant to ovarian reserve and assisted reproduction was done. Results Relevant papers were identified and assessed, and an attempt was made to understand, rationalize and explain the divergent views in this field of study. Seminal and original illustrations were employed. Conclusions FMR1 is a highly conserved gene whose interpretation and effect on outcomes remains controversial in the reproductive health setting. Recent re-evaluations of the commonly accepted normal range have yielded interesting tools for possibly explaining unexpected outcomes in assisted reproduction. Fragile X investigations should perhaps become more routinely assessed in the reproductive health setting, particularly following a failed treatment cycle where oocyte quality is thought to be a contributing factor, or in the presence of a surprise finding of diminished ovarian reserve in a young patient.

show abstract

Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

Cited by 145 publications

References 31 publications

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Effect of different levels of short-term feed intake on folliculogenesis and follicular fluid and plasma concentrations of lactate dehydrogenase, glucose, and hormones in Hu sheep during the luteal phase

The impact of FMR1 gene mutations on human reproduction and development: a systematic review

Contact Info

Product

Resources

About