Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection

Fertility and Sterility

Pane

et al. 2007

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114

102

Section: Resultsmentioning

confidence: 99%

Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures

Fertility and Sterility

Pane

et al. 2007

Self Cite

114

102

“…Moreover, DNA fragmentation in oocytes associated with apoptotic evidence might be one of the reasons for poor oocyte quality and lower fertility in aged mice [11]. A study on apoptosis in human oocytes collected in ICSI cycles, showed that apoptosis occurs also in human oocytes retrieved after hormonal stimulation and it is associated to the oocyte quality [12].…”

Section: From Follicular Apoptosis New Tools In Oocyte Selection?mentioning

confidence: 99%

New molecular markers for the evaluation of gamete quality

Fattouh

et al. 2013

J Assist Reprod Genet

Purpose Only 30 % of IVF cycles result in a pregnancy, so that multiple embryos need to be replaced, per treatment cycle, to increase pregnancy rates, resulting in a multiple gestation rate of 25 %. The use of new markers in the gamete selection, could reduce the number of the oocytes to be fertilized and embryos to be produced, but the tools to evidence the gamete competence remain unavailable and more studies are needed to identify bio-markers to select the best oocyte and sperm to produce embryos with higher implantation potentiality. Methods To define oocyte competence, the apoptosis of the surrounding cumulus cells and the oxygen consumption rates for individual oocytes before fertilization seems to provide a non-invasive marker of oocyte competence and hence a quantitative assessment of the reproductive potential for the oocyte. The chromatin integrity seems to be used also as biological marker of sperm competence, together with the morphological evaluation of large vacuoles in the head.Results The apoptosis rate of cumulus cells lower than 25 % and an higher oxygen consumption could be an evidence of an overall metabolic activity, related to a better fertilization ability and embryo cleavage quality. The apoptosis rate of the sperm chromatin, evaluated by direct Tunel in situ analysis, seems to be, also for the male gamete, a marker of competence and implantation potentiality, in particular when it is lower than 20 %. The evaluation of the presence of large vacuoles in the sperm head prior to perform ICSI seems to increase the implantation rate, but it is not associated to chromatin integrity. Conclusions The biological concept of competence appears unrelated to any morphological parameters, so that it is necessary to investigate new molecular markers in the gamete selection. Apoptosis of cumulus cells in the oocytes and spermatozoa, revealing the presence of large vacuoles, could help to determine the competence of the gamete to be fertilize.

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“…Previous data had shown DNA fragmentation in human unfertilized oocytes after ICSI, and the positivity to caspase-3 immunoassay suggested the occurrence of apoptosis. The high percentage of unfertilized oocytes bearing DNA fragmentation was correlated with fertilization failure [9]. In addition, Ruvolo et al [20].…”

Section: Discussionmentioning

confidence: 99%

“…Among the non-invasive tools, it is possible to include specific assays on cumulus cells; these cells are in close molecular communication with the female gamete and therefore represent an interesting investigative instrument in embryology [9]. Both the expression levels of some components of intracellular survival pathways and the apoptosis rate can be assessed in cumulus cells and utilized as potential markers of oocyte competence.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression and Apoptosis Levels in Cumulus Cells of Patients with Polymorphisms of FSHR and LHB Undergoing in Vitro Fertilization Program

Luparello

et al. 2017

Cell Physiol Biochem

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