Apoptosis in liver cancer (HepG2) cells induced by functionalized gold nanoparticles

Thirunavukkarasu, Arunachalam; Prabhu, D.; Geetha, R.; Govindaraju, K.; Manikandan, R.; Chinnasamy, Arulvasu; Ganesan, Singaravelu

doi:10.1016/j.colsurfb.2014.09.051

Cited by 81 publications

(33 citation statements)

References 40 publications

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“…Treated cells clearly indicates the possibility of apoptotic mechanisms leading to cell death which is evident by several morphological changes such as cell shrinkage, membrane blebbing, membrane integrity and formation of apoptotic bodies that are phagocytosed by surrounding cells or by phagocytes (Figures a–c). The results are in agreement with previous studies reporting the cytotoxic effects of AuNPs synthesized using Cajanus cajan on HepG2 cells and cytotoxicity of AuNPs synthesized using Couroupita guianensis flower extract onto HL‐60 cells . AuNPs induce toxicity through oxidative stress and inflammation by generating reactive oxygen species (ROS) in cancer cells.…”

Section: Resultssupporting

confidence: 92%

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Biosynthesis of gold nanoparticles using green roof species Portulaca grandiflora and their cytotoxic effects against C6 glioma human cancer cells

Thirunavukkarasu

Arockiaraj

Vijayaraghavan

2016

Env Prog and Sustain Energy

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Leaf and stem extracts of popular green roof vegetation, Portulaca grandiflora, were used for the synthesis of gold nanoparticles. The synthesized gold nanoparticles were characterized using UV–visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X‐ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. FTIR results indicated the presence of proteins and polyphenols in plant extracts which were responsible for the reduction and stabilization of gold nanoparticles. SEM and TEM images confirmed the formation of spherical, rhombus, triangular, tetragonal, pentagonal, rods and hexagonal shaped gold nanoparticles. The size of the particles was in the range of 8 and 72 nm. The antiproliferative effects of synthesized gold nanoparticles were assessed using C6 glioma human cancer cells. Results indicated that gold nanoparticles synthesized using leaf extracts (150 µg/mL) and stem extracts (200 µg/mL) of P. grandiflora decreased the viability of C6 glioma cell lines up to 50%. © 2016 American Institute of Chemical Engineers Environ Prog, 35: 1732–1740, 2016

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Section: Resultssupporting

confidence: 92%

“…AuNPs induce toxicity through oxidative stress and inflammation by generating reactive oxygen species (ROS) in cancer cells. The generated ROS in the cancer cells favours the DNA damage leading to apoptosis . Similar observation was made by Lin et al .…”

Section: Resultssupporting

confidence: 81%

Biosynthesis of gold nanoparticles using green roof species Portulaca grandiflora and their cytotoxic effects against C6 glioma human cancer cells

Thirunavukkarasu

Arockiaraj

Vijayaraghavan

2016

Env Prog and Sustain Energy

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“…Shen and coworkers reported that selenium nanocomposites using selenium dioxide plus potassium borohydride in ice bath without any surfactant or template and its UV range of about 380 nm [53]. Fesharaki and coworkers synthesized selenium nanoparticle using K. pneumoniae and reported an absorption maxima at 248 nm [54]. For selenium nanoparticles produced using Pseudomonas alcaliphila the observed absorption peak appeared at 280 nm [55].…”

Section: Resultsmentioning

confidence: 92%

“…Gold nanoparticles biologically synthesized showed different CC 50 values on normal cell lines i.e. rat kidney cell (200 lg/ml) [56,57] human stem cell (40 lg/ml) [34,58], human embryonic kidney cell (44 lg/ml) [59], mouse blood cells (36.49 lg/ml) [60] whereas gold nanoparticles synthesized using Cajanas cajan were subjected to assess toxic nature and the CC 50 obtained at the concentration of 246 lg/ml [54] and the flower extract Couroupita guianensis synthesized gold nanoparticles found to have CC 50 at 38.47 lg/ml [55]. Another significant nanomaterial which has been attractively studied for translational application is silver nanoparticles, their toxic nature differ according to their size, reductant and capping molecules involved in the formation of nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Green-Synthesis of Selenium Nanoparticles Using Garlic Cloves (Allium sativum): Biophysical Characterization and Cytotoxicity on Vero Cells

et al. 2016

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Nanotechnology has the potential to revolutionize a number of research fields, including biomedicine. Currently, the fabrication of biocompatible nanomaterials is of high interest worldwide. Little is known about the possible synthetic routes to fabricate selenium nanoparticles. In this research, we focused on the potential of a medicinally important plant species, garlic (Allium sativum), for the green synthesis of selenium nanoparticles. The garlic aqueous extracts acted as a capping and reducing agent in forming selenium nanoparticles. Green-synthesized selenium nanoparticles were characterized using UV-vis spectrophotometry, TEM, SEM, EDAX, FTIR and XRD analysis. Furthermore, green-fabricated and conventional chemically synthesized selenium nanoparticles were tested to evaluate their cytotoxicity against Vero cells. CC 50 values revealed that biologically synthesized selenium nanoparticles showed eco-friendly features and limited cytotoxicity if compared with chemically synthesized selenium nanoparticles.

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“…reported the IC50 value of Nigella sativa essential oil coated AuNPs against liver carcinoma cell was 28.37 mg/ml . The Cajanuscajan dried seed extract coated AuNPs showed IC50 value at 6 g/ml for 24 h exposure against HepG2 . On the other hand the IC50 value of Chitosan stabilized AuNPs was reported to be 75 ng/ml in 24 h exposure against MCF 7 cell lines .…”

Section: Resultsmentioning

confidence: 98%

Synthesis of Gold Nanoparticles Using Citrus macroptera Fruit Extract: Anti‐Biofilm and Anticancer Activity

et al. 2019

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Citrus macroptera (CM) fruit juice was used to reduce gold ions (Au3+) and to stabilize as‐formed gold nanoparticles (AuNPs). CM‐AuNPs exhibit surface Plasmon resonance (SPR) peak at 544 nm. Transmission Electron Microscope (TEM) study reveals that they are multi‐shaped, predominantly pseudo‐spherical with a diameter 20 nm. They have surface charge −30 mV and possess FCC lattice pattern. Particles could effectively inhibit the biofilm formation of Pseudomonas aeruginosa bacteria. Experimental study shows that CM‐AuNPs make the bacteria cripple to generate EPS and pyocyanin. AFM images of the structure of biofilm reveal that CM‐AuNPs indeed efficiently disrupt the biofilm structure. CM‐AuNPs in combination with Sub‐MIC dose of gentamicin against P. aeruginosa bacteria exhibit quite high efficiency. The cytotoxic effect of CM‐AuNPs against three human cancer cell line show that they are comparatively more efficient to regulate the growth of HepG2 (liver cancer cell line) than that of A 549 (adenocarcinogenic human alveolar basal epithial cell) and MDA‐MB 468 (breast cancer cell). Thus, the CM‐AuNPs could be treated as a potential anti‐biofilm and anti‐cancer agent.

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Apoptosis in liver cancer (HepG2) cells induced by functionalized gold nanoparticles

Cited by 81 publications

References 40 publications

Biosynthesis of gold nanoparticles using green roof species Portulaca grandiflora and their cytotoxic effects against C6 glioma human cancer cells

Biosynthesis of gold nanoparticles using green roof species Portulaca grandiflora and their cytotoxic effects against C6 glioma human cancer cells

Green-Synthesis of Selenium Nanoparticles Using Garlic Cloves (Allium sativum): Biophysical Characterization and Cytotoxicity on Vero Cells

Synthesis of Gold Nanoparticles Using Citrus macroptera Fruit Extract: Anti‐Biofilm and Anticancer Activity

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