Apoptosis in the rat mammary gland and ventral prostate: detection of cell death-associated genes using a coincident-expression cloning approach

Bielke, Wolfgang; Guo, Ke; Feng, Zhiwei; Bührer, Susanne; Saurer, Susanne; Friis, Robert R.

doi:10.1038/sj.cdd.4400220

Cited by 20 publications

(32 citation statements)

References 45 publications

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“…We have previously shown that DDC-4/sFRP-4 was upregulated in various tissues undergoing physiological apoptosis. 3,4,45 The new data show that DDC-4/sFRP-4 expression is a stimulus rather than a consequence of the apoptotic program in the mammary gland. Since DDC-4 is an sFRP and thus thought to sequester Wnt ligands, we propose a role for DDC-4/sFRP-4 in abrogating a Wnt-regulated survival pathway, the latter possibly signaling via PKB/Akt.…”

Section: Discussionmentioning

confidence: 90%

“…Mammary glands were minced on dry cell culture plates to fragments o1 mm 3 . The fragments were transferred to new culture dishes and allowed to dry for 5 min at room temperature after which Eagle's Dulbecco-modified medium (EDM) containing 10% fetal calf serum was added.…”

Section: Construction Of the Ddc-4/sfrp-4 Retroviral Vectormentioning

confidence: 99%

“…Equal loading was controlled by examination of ribosomal RNA bands following staining with acridine orange as previously described. 3 Probes for hybridization were prepared using the Random Primed Labeling Kit (Roche Diagnostics, Rotkreuz, Switzerland) including 32 P-dCTP (800 Ci/mM; Amersham International, Little Chalfont, England) in the reaction and hybridization was carried out as previously described. 3 In situ hybridization, immunohistochemical detection of DDC-4/sFRP-4, incorporated BrdU and activated (cleaved) caspase-3…”

Section: Isolation Of Rna and Northern Blottingmentioning

confidence: 99%

“…1,2 We were interested in identifying genes involved in the control and execution of mammary gland apoptosis. 2,3 Since the process of mammary involution is complex, comprising a tissue remodeling program that proceeds in parallel with programmed cell death, a differential display coincidence (DDC) method was employed to recognize genes upregulated in common between two models of physiological apoptosis: the involuting mammary gland and the prostate following castration. 3 One of the most interesting genes isolated, DDC-4, showed striking homology to the ligand-binding domain of several frizzled family, 7-pass transmembrane receptors.…”

Section: Introductionmentioning

confidence: 99%

“…2,3 Since the process of mammary involution is complex, comprising a tissue remodeling program that proceeds in parallel with programmed cell death, a differential display coincidence (DDC) method was employed to recognize genes upregulated in common between two models of physiological apoptosis: the involuting mammary gland and the prostate following castration. 3 One of the most interesting genes isolated, DDC-4, showed striking homology to the ligand-binding domain of several frizzled family, 7-pass transmembrane receptors. DDC-4 was subsequently recognized as encoding a product belonging to the secreted frizzled-related protein (sFRP) subfamily, 4 and is referred to here as DDC-4/sFRP-4.…”

Section: Introductionmentioning

confidence: 99%

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Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

et al. 2003

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Using differential display, we isolated DDC-4, a secreted frizzled-related protein (sFRP), which is induced in the physiological apoptosis of hormonally regulated, reproductive tissues such as mammary gland, prostate, corpus luteum and uterus. The role of this gene in apoptosis was studied in animals overexpressing ectopic DDC-4/sFRP-4. Transgenic mice bearing the DDC-4/sFRP-4 cDNA under the control of the MMTV-LTR promoter showed lactational insufficiency and many apoptotic cells in the alveoli between day 19 of pregnancy and day 4 of lactation as demonstrated by TUNEL reaction and the presence of activated caspase-3. We performed a PKB/Akt kinase assay and studied several of its substrates using phosphorylation-specific antibodies to show reduced phosphorylation in PKB/Akt itself, as well as in glycogen synthetase kinase-3b (GSK-3b), BAD, and Forkhead. Taken together, our results show a role for DDC-4/sFRP-4 in abrogating an epithelial cell survival pathway at the onset of mammary gland involution.

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Section: Discussionmentioning

confidence: 90%

Section: Construction Of the Ddc-4/sfrp-4 Retroviral Vectormentioning

confidence: 99%

Section: Isolation Of Rna and Northern Blottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

et al. 2003

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Transcriptionally mediated gene targeting of gas1 to glioma cells elicits growth arrest and apoptosis

Zamorano

Lamas

Vergara

et al. 2002

J of Neuroscience Research

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Induction of growth arrest-specific genes (gas1) prevents cell proliferation and/or leads to apoptosis in different cell types. In neurons, it has been recently reported that mild excitotoxic neuronal death is associated with gas1 induction, and that overexpression of Gas1 induces apoptosis in terminally differentiated neurons or in proliferating neuroblastoma cells. In the present study, we have analysed the effects of the transcriptionally mediated targeting of gas1 to C6 rat glioma cells. Expression of Gas1 decreased glial proliferation and induced C6 cell apoptosis. While the identity of the caspase(s) responsible for Gas1-induced apoptosis in neurons has remained elusive, in C6 glioma cells, overexpression of Gas1 reproducibly activated caspase-3. Our results support the concept of targeted expression of gas1 as a potentially useful gene therapy strategy in the treatment of human gliomas.

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Gas1 Is Induced during and Participates in Excitotoxic Neuronal Death

Mellström

Ceña

Lamas

et al. 2002

Molecular and Cellular Neuroscience

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Apoptosis in the rat mammary gland and ventral prostate: detection of cell death-associated genes using a coincident-expression cloning approach

Abstract: Apoptosis plays a striking role in the hormone-dependent

Cited by 20 publications

References 45 publications

Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution

Transcriptionally mediated gene targeting of gas1 to glioma cells elicits growth arrest and apoptosis

Gas1 Is Induced during and Participates in Excitotoxic Neuronal Death

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