Apoptosis induced by N6-substituted derivatives of adenosine is related to intracellular accumulation of corresponding mononucleotides in HL-60 cells

Mlejnek, Petr; Doležel, Petr

doi:10.1016/j.tiv.2005.06.023

Cited by 46 publications

(70 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This strategy has been employed to develop potential anticancer drugs. Thus, it was found that the proapoptotic effects of N 6 -substituted derivatives of adenosine are related to their intracellular conversion into corresponding mononucleotides by ADK (Mlejnek and Dolezel, 2005). Vidarabine (9-b-D-ribofuranosyladenine or AraA) is an analog of adenosine containing D-arabinose instead of D-ribose and was originally considered as an anticancer drug (LePage et al, 1973).…”

Section: Pharmacologymentioning

confidence: 99%

Adenosine Kinase: Exploitation for Therapeutic Gain

2013

View full text Add to dashboard Cite

Section: Pharmacologymentioning

confidence: 99%

Adenosine Kinase: Exploitation for Therapeutic Gain

2013

View full text Add to dashboard Cite

“…It has also been shown that kinetin as well as isopentenyladenine, benzyladenine and 6-dimethyladenine but not zeatin are potent inhibitors of the proliferation of myelomonocytoid leukemia cells: ML-1, NB4, U937 [64]. Pro-apoptotic effects of kinetin and other N6-substituted adenines are related to their intracellular conversion into corresponding mononucleotides, which eventually trigger apoptosis [65].…”

Section: Kinetin Propertiesmentioning

confidence: 99%

Kinetin—A multiactive molecule

Barciszewski

Massino²,

Clark

2007

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

“…minor seedlings. Kinetin-induced PCD process accompanied with changes in the number of cells in G1 and G2 phases of the cell cycle, in the activity of cellular dehydrogenases, in the ROS production, amount of cytosolic calcium ions, conductivity of cell electrolytes secreted from roots to the culture media and in the morphology of cells and roots was almost completely inhibited by adenine, an inhibitor of phosphorybosyl transferase (Mlejnek and Doležel 2005), and mannitol, the ROS scavenger (Jennings et al 1998). …”

Section: Introductionmentioning

confidence: 99%

Kinetin induces cell death in root cortex cells of Vicia faba ssp. minor seedlings

2012

View full text Add to dashboard Cite

The double fluorescence staining with acridine orange and ethidium bromide (AO/EB) revealed that treatment of Vicia faba ssp. minor seedlings with kinetin-induced programmed cell death (PCD) in root cortex cells. Kinetin-induced cell death reflected by the morphological changes of nuclei including their invagination, volume increase, chromatin condensation and degradation as well as formation of micronuclei showed by AO/EB and 4,6-diamidino-2-phenylindol staining was accompanied by changes including increase in conductivity of cell electrolytes secreted to culture media, decrease in the number of the G1- and G2-phase cells and appearance of fraction of hypoploid cells as the effect of DNA degradation without ladder formation. Decrease in the number of mitochondria and in the activity of cellular dehydrogenases, production of reactive oxygen species (ROS), appearance of small and then large lytic vacuoles and increase in the amount of cytosolic calcium ions were also observed. The PCD was also manifested by increased width and weight of apical fragments of roots as well as decreased length of cortex cells which led to shortening of the whole roots. The kinetin-induced PCD process was almost completely inhibited by adenine, an inhibitor of phosphoribosyl transferase, and mannitol, an inhibitor of ROS production. These cell-death hallmarks and pathway of this process suggested that the induction of kinetin-specific vacuolar type of death, expressed itself with similar intensity on both morphological and metabolic levels, was a transient protecting whole roots and whole seedlings against elimination.

show abstract

Apoptosis induced by N6-substituted derivatives of adenosine is related to intracellular accumulation of corresponding mononucleotides in HL-60 cells

Cited by 46 publications

References 16 publications

Adenosine Kinase: Exploitation for Therapeutic Gain

Adenosine Kinase: Exploitation for Therapeutic Gain

Kinetin—A multiactive molecule

Kinetin induces cell death in root cortex cells of Vicia faba ssp. minor seedlings

Contact Info

Product

Resources

About