Apoptosis inducer NGFI-B is degraded by the proteasome and stabilized by treatment with EGF

Strom, B.; Paulsen, Ragnhild E.

doi:10.1016/j.bbrc.2011.12.132

Cited by 10 publications

(12 citation statements)

References 32 publications

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“…The present study, for the first time, demonstrates that IFI27 protein stability was prolonged by EGF treatment in keratinocytes. By the mechanism of stabilizing proteins to enhance their activities, two other growth‐related proteins, NGFI‐B and EGR1 have also been found to be increased and stabilized by EGF . It is still not clear how EGF increase protein stability; however, our data provide evidence to support the role of IFI27 in pathogenesis of psoriasis, specially in hyperproliferation of keratinocytes.…”

Section: Discussionmentioning

confidence: 52%

IFI27, a novel epidermal growth factor‐stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes

et al. 2015

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Section: Discussionmentioning

confidence: 52%

IFI27, a novel epidermal growth factor‐stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes

et al. 2015

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“…Epidermal growth factor (EGF), an activating signal for ERK2, causes phosphorylation of Nur77 at threonine 142 [36], a phosphorylation site that stabilizes Nur77 [37], consistent with the phosphorylation-mediated stability of other nuclear receptors. Interestingly, EGF enhances the interaction of Nur77 with prolyl isomerase Pin1, and the isomerization of phosphorylated Nur77 requires ERK2- (and JNK1)-mediated phosphorylation [38].…”

Section: Nr4a Receptors and Mapkmentioning

confidence: 99%

The interplay of NR4A receptors and the oncogene–tumor suppressor networks in cancer

Beard

Tenga

Chen

2015

Cellular Signalling

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Nuclear receptor (NR) subfamily 4 group A (NR4A) is a family of three highly homologous orphan nuclear receptors that have multiple physiological and pathological roles, including some in cancer. These NRs are reportedly dysregulated in multiple cancer types, with many studies demonstrating pro-oncogenic roles for NR4A1 (Nur77) and NR4A2 (Nurr1). Additionally, NR4A1 and NR4A3 (Nor-1) are described as tumor suppressors in leukemia. The dysregulation and functions of the NR4A members are due to many factors, including transcriptional regulation, protein-protein interactions, and post-translational modifications. These various levels of intracellular regulation result from the signaling cross-talk of the NR4A members with various signaling pathways, many of which are relevant to cancer and likely explain the family members' functions in oncogenesis and tumor suppression. In this review, we discuss the multiple functions of the NR4A receptors in cancer and summarize a growing body of scientific literature that describes the interconnectedness of the NR4A receptors with various oncogene and tumor suppressor pathways.

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“…Western analysis showed that NGFI‐B protein was present in cultures of rat cerebellar granule neurons at DIV 4/5 in concordance with former studies (Mathisen et al, 2011). The applied antibody targeting NGFI‐B had previously been validated (Mathisen et al, 2011; Strom and Paulsen, 2012) and a positive control was included to identify correct band size (results not included). Three siRNA sequences were tested, and the sequence specified in Section 2 was found to be effective.…”

Section: Resultsmentioning

confidence: 99%

“…NGFI-B is rapidly induced after stimuli (Watson and Milbrandt, 1989) and has a short half-life (Strom and Paulsen, 2012) which makes it a suitable candidate to target with siRNA. To provide reliable results this depends on high transfection efficiency.…”

Section: Discussionmentioning

confidence: 99%

Calcium‐induced apoptosis of developing cerebellar granule neurons depends causally on NGFI‐B

Austdal

Mathisen

Løberg³

et al. 2016

Intl J of Devlp Neuroscience

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Immediate early gene nerve growth factor-induced clone B (NGFI-B), a nuclear receptor important for differentiation and apoptosis, is expressed in mice and rat cerebellum from an early stage of postnatal development. Following apoptotic stimuli NGFI-B translocates to mitochondria to initiate cell death processes. Controlled cell death is critical for correct cerebellar development. Immunohistochemical analysis of NGFI-B in sections of mice cerebella showed NGFI-B to be expressed in granule neurons in vivo at a time (P8-11) when apoptosis is known to occur. The importance of NGFI-B for apoptosis of cultured rat cerebellar granule neurons was investigated by inducing apoptosis with calcium ionophore A23187 (CaI, 0.1μM). Imaging studies of gfp-tagged NGFI-B confirmed that mitochondrial translocation of NGFI-B occurred following treatment with CaI and was reduced by addition of 9-cis-retinoic acid (1μM), a retinoid X receptor (RXR) agonist that prevents dimerization of RXR and NGFI-B that is known to occur before translocation. Consequently, 9-cis-retinoic acid partly reduced cell death. To address the causality of NGFI-B in apoptosis further, knock-down by siRNA was performed and it removed 85% of the NGFI-B protein. This resulted in a complete inhibition of apoptosis after CaI exposure. Together these findings suggest that NGFI-B plays a role in controlling correct cerebellar development.

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Apoptosis inducer NGFI-B is degraded by the proteasome and stabilized by treatment with EGF

Cited by 10 publications

References 32 publications

IFI27, a novel epidermal growth factor‐stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes

IFI27, a novel epidermal growth factor‐stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes

The interplay of NR4A receptors and the oncogene–tumor suppressor networks in cancer

Calcium‐induced apoptosis of developing cerebellar granule neurons depends causally on NGFI‐B

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