Apoptosis-Inducing Factor and Colon Cancer

Millán, Alejandro; Huerta, Sergio

doi:10.1016/j.jss.2007.05.020

Cited by 67 publications

(46 citation statements)

References 69 publications

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“…Usually, after apoptotic stimuli, AIF is released into the cytosol from the mitochondrial intermembrane where it interacts with cyclophilin A to become a DNase. Subsequently, when AIF is translocated to the nucleus, this may contribute to DNA fragmentation leading to cell death (17). A relatively high level of AIF (>20%) was found for the control group (Fig.…”

Section: Morphology Of the Colon Mucosa Differs With Aging Kwon Andmentioning

confidence: 90%

Implications of cleaved caspase 3 and AIF expression in colorectal cancer based on patient age

Perraud¹

2012

Oncol Rep

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Abstract.A high incidence of colorectal cancer (CRC) has been established in the elderly population. Apoptosis is a key event in maintaining colon homeostasis, both in aging as well as in cancer prevention. Here, we report that colon morphology is affected during the aging process: crypt loss (P=0.045) and increasing distances between crypts (P=0.0001678) were observed, associated with a tendency for mucosa reduction (P=0.083). In addition, our results show that apoptosis plays a determining role on the effect of aging during CRC. Increased expression of cleaved caspase 3 (the key factor implicated in the caspase-dependent pathway; P=0.026 for non-tumor tissues, P=0.0013 for tumor tissues) and AIF (implicated in the caspase-independent pathway; P=0.037) in tissue from elderly patients has been observed. Furthermore, elderly patients respond better to chemotherapy than younger ones (P=9.27x10 -5 ). These results suggest that patient age should be taken into account to adapt treatment of CRC.

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Section: Morphology Of the Colon Mucosa Differs With Aging Kwon Andmentioning

confidence: 90%

Implications of cleaved caspase 3 and AIF expression in colorectal cancer based on patient age

Perraud¹

2012

Oncol Rep

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“…It plays a vital role in cellular homeostasis by controlling cell proliferation (Millan and Huerta 2009;Ramasamy et al 2013). Numerous plants and their constituents are known to possess apoptosis inducing potential (Hsieh et al 2006;Hsu et al 2011;Kim et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…(Kerr et al 1972). During apoptosis, caspases get activated and induce several molecular cascades which results in cell death (Allen et al 1997;Hu and Kavanagh 2003;Wang et al 2005;Elmore 2007;Millan and Huerta 2009). Apoptosis is the main mechanism which maintains tissue homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

Kumar

Kaur

Kumar

et al. 2015

Physiol Mol Biol Plants

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Two non-polar fractions viz. hexane (Hex-LI) and chloroform fraction (CHCl 3 -LI) of Lawsonia inermis were studied for their antiproliferative potential in various cancer cell lines viz. HeLa, MCF-7, A549 and C6 glioma cells. Both the fractions showed more than 60 % of growth inhibition in all the tested cell lines at highest tested concentration. In clonogenic assay, different concentrations of Hex-LI and CHCl 3 -LI decreased the number and size of colonies as compared to control in HeLa cells. The apoptotic effects as nuclear condensation, fragmentation were visualized with Hoechst-33342 staining of HeLa cells using confocal microscope. Both fractions induced apoptotic cell death in human cervical carcinoma (HeLa) cells as evident from flow cytometric analysis carried out using Annexin V-FITC and propidium iodide dyes. CHCl 3 -LI treated cells significantly induced apoptosis (25.43 %) in comparison to control. Results from Neutral Comet assay demonstrated that both fractions induced double stranded breaks (DSB's) in HeLa cells. Our data indicated that Hex-LI and CHCl 3 -LI treated cells showed significant increase of 32.2 and 18.56 % reactive oxygen species (ROS) levels in DCFH-DA assay respectively. Further, experimental studies to decipher exact pathway via which these fractions induce cell death are in progress.

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“…Despite recent improvements in the treatment of colorectal cancer, the response rate still remains low (4). A key factor in the response of cancer cells to chemotherapeutic drugs is the activation of the apoptotic pathway, which is often impaired in chemoresistant colon cancer cells (5). Many chemotherapeutic drugs including cisplatin induce cytotoxic effects in cancer cells mainly through programmed cell death or apoptosis (6), which is characterized by membrane blebbing, shrinking of cells and their organelles, DNA fragmentation, and finally cell disintegration (7).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis-Inducing Factor and Colon Cancer

Cited by 67 publications

References 69 publications

Implications of cleaved caspase 3 and AIF expression in colorectal cancer based on patient age

Implications of cleaved caspase 3 and AIF expression in colorectal cancer based on patient age

Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

Vitamin C increases the apoptosis via up-regulation p53 during cisplatin treatment in human colon cancer cells

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