Apoptosis Induction Activity and Inhibition of MCF-7 Cell Proliferation in Active Isolate of Butterfly Pea Flower (Clitoria ternatea L.)

Abstract

Clitoria ternatea L. is one of the plants whose all parts have functional benefits for the human body. The flower petals are reported to be useful as anticancer. This study aims to determine the activity of inhibiting cell proliferation, and apoptotic induction activity and identify the chemical content of isolates from Clitoria ternatea L. Analysis of the results of the MTT assay test ethanol extract of butterfly pea flower has an IC50 value of 17.46 g/mL, CTEE has an IC50 value of 13, 19 g/mL, isolate 1 and isolate 2 of EAFCT had IC50 values of 6.79 and 7.12 g/mL. The results obtained were categorized as potent cytotoxic. Isolation of EAFCT compounds using radial system chromatography then analyzed GC-MS EAFCT isolates containing pyridine hydrochloride (51.42 %) and pentanal (2.79 %) and identified compounds using UV-Vis spectrophotometry and FTIR. The results of the cell proliferation inhibition test used a series of concentrations of IC50, ⅟2 IC50, ⅟4 IC50 and ⅟8 IC50, the CTEE obtained a doubling time of 178.47; 160.86; 151.18; 150.09, EAFCT is 188.53; 164.37; 156.82; 154.79, isolate 1 EAFCT is 212.96; 174.64; 148.53; 145.82 and isolates 2 EAFCT were 190.62; 168.23; 150.24; 148.94. Analysis of the apoptotic potential test results can be concluded from CTEE, EAFCT, isolates 1 and 2 EAFCT have apoptotic potential. HIGHLIGHTS The Clitoria ternatea 1 and 2 ethyl acetate fractions had a cytotoxic effect on MCF-7 cells with IC50 values of 6.79 and 7.12 μg/mL and inhibited the growth rate of MCF-7 cells with doubling times of 212.96 and 190.62 hours Clitoria ternatea 1 and 2 isolates' ethyl acetate fraction induced apoptosis in MCF-7 cells, as indicated by the presence of orange fluorescent cells, indicating that the cells had died The Ethyl acetate fraction of Clitoria ternatea isolate contains compounds with anticancer potential, including Pyridine hydrochloride and Pentanal GRAPHICAL ABSTRACT