Apoptosis induction of <i>Centella asiatica</i> on human breast cancer cells

Abstract

The present study evaluated the ability of methanolic extract of Centella asiatica (Linn) Urban (Umbelliferae) to induce apoptosis in different cancer cell lines. MCF-7 cells emerged as the most sensitive cell line for in vitro growth inhibitory activity. C. asiatica extract induced apoptosis in MCF-7 cells as indicated by nuclear condensation, increased annexin staining, loss of mitochondrial membrane potential and induction of DNA breaks identified by TUNEL reactivity. It is possible that the use of C. asiatica extract as a component in herbal medicines could be justifiable.

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

“…Additionally, C. asiatica induced gastric ulcers and promoted epithelial cell proliferation in rats (28). However, C. asiatica has also shown anti-proliferative properties in solid cancers, such as melanoma, breast, liver and gastric cancers, as well as in keratinocytes (29)(30)(31)(32)(33)). An anti-proliferative effect on keratinocytes was shown to be highest following treatment with 18.4±0.6 µg/ml C. asiatica extract (33).…”

Edible freshwater macrophytes: a source of anticancer and antioxidative natural products—a mini-review

“…Sequential fractionation of active extracts with organic solvents, for example, petroleum ether, acetone, dichloromethane, ethyl acetate, butanol and methanol (Khlifi et al 2013;Tagne et al 2014) has also been reported. The cytotoxicity assay most widely used for estimating the anticancer potential of EFM is the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Babykutty et al 2008;Fan et al 2014;Liu et al 2014;Park et al 2005), but other assays such as the sulphorhodamine B (SRB), neutral red, and lactate dehydrogenase (LDH) leakage assays have also been used (Aboul-Enein et al 2014;Prasad et al 2005;Rose et al 2005). The MTT assay gives an indication of whole cell cytotoxicity.…”

“…Solvents such as methanol, ethanol, chloroform and water are widely used to extract anticancer compounds from EFM (Babykutty et al 2008;DantasSantos et al 2012;Mukherjee et al 2009;Pittella et al 2009). Sequential fractionation of active extracts with organic solvents, for example, petroleum ether, acetone, dichloromethane, ethyl acetate, butanol and methanol (Khlifi et al 2013;Tagne et al 2014) has also been reported.…”

“…The assay can be readily performed on a wide range of cell lines, with high reliability and versatility (McCauley et al 2013). Cancer cell lines used to test the cytotoxicity of EFM included human breast cancer (MDA-MB-231; MCF-7), human hepatocellular carcinoma (HepG2), human lung carcinoma (A549), human colon adenocarcinoma (HT29; SW480), human cervix adenocarcinoma (HeLa), rat glioma (C 6 ), and mouse melanoma (B 16 F 1 ) cell lines (Babykutty et al 2008;Boyd et al 2006;Pittella et al 2009;Rose et al 2005).…”

A Review on Medicinal Properties of Centella Asiatica

“…Against the cell lines of human breast cancer (MDA MB-231), mouse melanoma (B16F1), and rat glioma (C6), the aqueous extract of C. asiatica had shown inhibitory activity with IC50 values of 698.0, 648.0, and 1000.0 µg/mL, respectively [16]. The methanolic extract of C. asiatica (Linn) showed inhibitory effect on MCF-7 cell lines, and induced apoptosis in MCF-7 cells as indicated by nuclear condensation, increased annexin staining, loss of mitochondrial membrane potential, and induction of DNA breaks was identified by TUNEL reactivity [17]. The effect of C. asiatica juice was checked on human HepG2 cell line using MTT assay, and it showed cytotoxic effects on tumor cells in a dose-dependent manner.…”