Apoptosis, necroptosis, and pyroptosis as alternative cell death pathways induced by chemotherapeutic agents?

Gielecińska, A.; Kciuk, M.; Yahya, E.-B.; Ainane, T.; Mujwar, S.; Kontek, R.

doi:10.1016/j.bbcan.2023.189024

Cited by 21 publications

(5 citation statements)

References 166 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…41 The fragmentation of DNA and abnormal cell size, causing cells to constrict and shrink, are two traits that indicate the start of apoptosis in cancerous cells. 42 When compared to untreated cancer cells, cells with the IC 50 concentration of protopine showed abnormal morphology, exhibiting shrinkage and an unclear nucleus shape. This is a result of DNA fragmentation of tumor cells, which leads to apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic Activity of Protopine against Human Breast Cancer Cell Lines: An in vitro Study

Li,

Sivapragasam,

Arulselvan

et al. 2024

Ind. J. Pharm. Edu. Res

View full text Add to dashboard Cite

Background: Breast Cancer continues to rank among all cancer types as the most dangerous malignancies, accounting for a significant portion of cancer-associated mortalities among women globally. Additionally, the number of breast cancer cases that are identified each year is rising globally. After surgery or radiation, the typical therapies entail chemotherapy which is followed by endocrine therapy. However, breast cancer is extremely resistant to therapies, which causes it to recur. As a result, emphasis is being placed on the development of complementary medicines with fewer adverse effects that are obtained from plants. An isoquinoline alkaloid, Protopine, possesses an array of biological properties, including anti-tumor efficacy against several malignancies. Materials and Methods: In this work, the anti-carcinogenic property of Protopine against the MDA-MB-231 cells has been investigated. The impact of Protopine on cell growth, apoptosis, ROS accumulation, and apoptotic marker levels was investigated. Doxorubicin was employed as the positive control drug in the studies. Results:The effect of Protopine on cell growth demonstrated its cytotoxic property was elevated dose-dependently and IC 50 concentration was selected for additional work. The AO/EB and DAPI staining was performed to examine morphological alterations concerning apoptotic cell death. Additionally, Protopine augmented the ROS accumulation and induced DNA damage in Protopine-treated cells. The caspase levels were also increased upon Protopine treatment. Discussion and Conclusion:The outcomes highlight that Protopine remarkably inhibited cell growth by augmenting the ROS levels, inducing DNA fragmentation and apoptosis, leading to cell death by caspase-dependent pathway. Thus, Protopine could be possibly employed as an effective anti-cancer alternative for breast cancer treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Apoptotic Activity of Protopine against Human Breast Cancer Cell Lines: An in vitro Study

Li,

Sivapragasam,

Arulselvan

et al. 2024

Ind. J. Pharm. Edu. Res

View full text Add to dashboard Cite

show abstract

“…Normally, cells that cannot neutralize and repair toxin-induced damage undergo regulated cell death (RCD), such as apoptosis, which exhibits significant morphological changes and is activated by specific Cysteinyl aspartate-specific proteinases (Caspases) and signaling pathways [ 12 ]. However, due to its anti-inflammatory and immunosuppressive effects, apoptosis could not account for the inflammation after toxin exposure [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to apoptosis, regulated necrosis leads to the release of immunogenic intracellular molecules, which in turn triggers inflammation [ 16 ]. Regulated necrosis commonly includes necroptosis, pyroptosis, and ferroptosis [ 12 ]. Pyroptosis is the activation of multiple Caspases, including Caspase1, mediated by inflammasomes, causing splicing and aggregation of various Gasdermin family members, leading to cell perforation and ultimately cell death.…”

Section: Introductionmentioning

confidence: 99%

Chlormequat Chloride Inhibits TM3 Leydig Cell Growth via Ferroptosis-Initiated Inflammation

Wang,

Kang,

Guo

et al. 2024

Cells

View full text Add to dashboard Cite

Ferroptosis hallmarked by lipid peroxidation and iron homeostasis imbalance is involved in the occurrence and development of various diseases. The plant growth regulator chlormequat chloride (CCC) can contribute to the causality and exacerbation of reproductive disorders. However, the mechanism by which CCC may cause Leydig cell attenuation remains poorly understood. In this study, TM3 Leydig cells were used to investigate the inhibitory effect of CCC on cell growth and its possible mechanism. The results showed that CCC caused apoptosis, pyroptosis, ferroptosis and necroinflammation in TM3 cells. By comparing the effects of ferroptosis inhibitor Ferrostatin-1 (Fer-1) and pan-Caspase inhibitor Z-VAD-FMK (ZVF) on lipid peroxidation and Caspase-mediated regulated cell death (RCD), we found that Fer-1 was better at rescuing the growth of TM3 cells than ZVF. Although ZVF reduced mitochondrial ROS level and inhibited the activation of Caspase3 and Caspase1, it could not significantly ameliorate lipid peroxidation and the levels of IL-1β and HMGB1 like Fer-1. Therefore, ferroptosis might be a key non apoptotic RCD mode responsible for CCC-driven inflammation, leading to weakened viability and proliferation of TM3 cells. In addition, overexpression of ferritin light chain (FTL) promoted the resistance of TM3 cells to CCC-induced ferroptosis-mediated inflammation and to some extent improved the inhibition of viability and proliferation. Altogether, ferroptosis-initiated inflammation might play a key role in CCC-impaired TM3 cell growth.

show abstract

“…It involves the multiplication and expansion of normal, healthy cells. The disruption of this system can lead to the development and multiplication of aberrant cells, specifically malignant cells [4]. Moreover, the process of destabilizing, degrading, and invading healthy tissues in cancer disrupts the ongoing growth of cells [5].…”

Section: Introductionmentioning

confidence: 99%

Recent Advancements in Metallic Au- and Ag-Based Chitosan Nanocomposite Derivatives for Enhanced Anticancer Drug Delivery

El-Meligy,

Abd El-Monaem,

Eltaweil

et al. 2024

Molecules

View full text Add to dashboard Cite

The rapid advancements in nanotechnology in the field of nanomedicine have the potential to significantly enhance therapeutic strategies for cancer treatment. There is considerable promise for enhancing the efficacy of cancer therapy through the manufacture of innovative nanocomposite materials. Metallic nanoparticles have been found to enhance the release of anticancer medications that are loaded onto them, resulting in a sustained release, hence reducing the dosage required for drug administration and preventing their buildup in healthy cells. The combination of nanotechnology with biocompatible materials offers new prospects for the development of advanced therapies that exhibit enhanced selectivity, reduced adverse effects, and improved patient outcomes. Chitosan (CS), a polysaccharide possessing distinct physicochemical properties, exhibits favorable attributes for controlled drug delivery due to its biocompatibility and biodegradability. Chitosan nanocomposites exhibit heightened stability, improved biocompatibility, and prolonged release characteristics for anticancer medicines. The incorporation of gold (Au) nanoparticles into the chitosan nanocomposite results in the manifestation of photothermal characteristics, whereas the inclusion of silver (Ag) nanoparticles boosts the antibacterial capabilities of the synthesized nanocomposite. The objective of this review is to investigate the recent progress in the utilization of Ag and Au nanoparticles, or a combination thereof, within a chitosan matrix or its modified derivatives for the purpose of anticancer drug delivery. The research findings for the potential of a chitosan nanocomposite to deliver various anticancer drugs, such as doxorubicin, 5-Fluroacil, curcumin, paclitaxel, and 6-mercaptopurine, were investigated. Moreover, various modifications carried out on the chitosan matrix phase and the nanocomposite surfaces to enhance targeting selectivity, loading efficiency, and pH sensitivity were highlighted. In addition, challenges and perspectives that could motivate further research related to the applications of chitosan nanocomposites in cancer therapy were summarized.

show abstract

Apoptosis, necroptosis, and pyroptosis as alternative cell death pathways induced by chemotherapeutic agents?

Cited by 21 publications

References 166 publications

Apoptotic Activity of Protopine against Human Breast Cancer Cell Lines: An in vitro Study

Apoptotic Activity of Protopine against Human Breast Cancer Cell Lines: An in vitro Study

Chlormequat Chloride Inhibits TM3 Leydig Cell Growth via Ferroptosis-Initiated Inflammation

Recent Advancements in Metallic Au- and Ag-Based Chitosan Nanocomposite Derivatives for Enhanced Anticancer Drug Delivery

Contact Info

Product

Resources

About