Apoptosis of polymorphonuclear leukocytes of the juvenile bovine mammary gland during induced influx

Sládek, Z.; Rysanek, D.

doi:10.1051/vetres:2000139

Cited by 33 publications

(47 citation statements)

References 28 publications

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“…The resolution of mammary gland injury was more rapid after PBS treatment than after LPS treatment. This result is consistent with our earlier findings [23,24].…”

Section: Discussionsupporting

confidence: 94%

“…This decrease became apparent at 48 h in PBS-induced and at 72 h in LPS-induced mammary gland injury. These changes closely correlate with the clearance of apoptotic neutrophils, as determined in our previous paper in which the highest counts of apoptotic neutrophils and myeloperoxidase-positive mononuclear phagocytes were found at 48 and 72 h in PBS-induced and LPS-induced mammary gland injury, respectively [23,24]. This difference can be attributed to LPS which prolonged the influx of neutrophils and delayed the onset of neutrophil apoptosis.…”

Section: Discussionsupporting

confidence: 85%

“…Influx of leukocytes can also be induced by intramammary treatment with PBS [4,20]. The influx in which neutrophil leukocytes predominate, culminates within 24 h. Mammary gland injury is resolved after 72 to 96 h and the major role is played by apoptosis and phagocytosis of apoptotic neutrophils by mononuclear phagocytes [23,24]. The elimination of neutrophils is associated with shifts in differential counts of other cell types.…”

Section: Discussionmentioning

confidence: 99%

“…The resolution of LPS-induced mammary gland inflammation is characterised by significant interactions among inflammatory cells which result in the subsidence of influx by apoptosis of neutrophils. Mononuclear phagocytes of the cavity system of the mammary gland participates in the clearance of apoptotic neutrophils by phagocytosis [23,24]. Mononuclear phagocytes of heifers mammary glands include two morphologically distinct cell types.…”

Section: Introductionmentioning

confidence: 99%

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Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Sládek¹,

Rysanek²,

Faldyna

2002

Vet. Res.

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-The object of the study was the comparative assessment of phagocyte activation during initiation and resolution of mammary gland injury induced by lipopolysaccharide (LPS) or buffered salt solution (PBS) on the basis of the CD14 receptor positivity. The experiments were carried out in 15 clinically normal Holstein × Bohemian Red Pied crossbred heifers, aged 14 to 18 months. Noninflammatory and inflammatory mammary gland injury were induced by intramammary administration of PBS (10 mL) and LPS (10 mL, 1 µg/mL), respectively. Samples of the cell populations were obtained by mammary lavages at 24 h intervals. Flow cytometry was used to determine the CD14+ neutrophils, monocytes, and macrophages. The percentage of CD14+ neutrophils was only 1.2% and 1.3% 24 h after the treatment with PBS and LPS, respectively. The resolution was accompanied by an increase in proportion of CD14+ neutrophils. The proportion of CD14+ neutrophils returned to initial values in the PBS-treated, but not in the LPS-treated mammary glands till 96 h. Percentage of CD14+ monocytes increased after 24 h and the effect was more pronounced in the LPS-treated than in the PBS treated mammary glands (P < 0.05). The percentage of CD14+ macrophages decreased highly significantly at 24 h in the LPS-treated, but not in the PBS-treated mammary glands (P < 0.01). The resolution of mammary gland injury (48 to 96 h) was characterised by an increase in CD14+ macrophages proportion, which was greater in the LPS-treated than PBS-treated mammary glands (P < 0.01). The activation of macrophages during resolution of mammary gland injury can be interpreted as an important mechanism of restitution. Résumé -Activation des phagocytes au cours de l'initiation et de la résolution de lésion de la glande mammaire induite par des lipopolysaccharides chez des génisses. L'objet de la présente étude est l'analyse de l'activation des phagocytes au cours de l'initiation et de la résolution de lésion de la glande mammaire induite par des lipopolysaccharides (LPS), ou par la solution physiologique (PBS), et cela sur la base de la positivité du CD14 récepteur. L'essai fut réalisé sur 15 génisses, cliniquement en bonne santé, issues du croisement des races Holstein × Pie rouge tchèque, âgées de 14 à 18 mois. Des lésions non-inflammatoires et inflammatoires de la glande mammaire ont été induites par l'administration intra-mammaire de PBS (10 mL) et LPS (10 mL, 1 µg/mL), respectivement. Les échantillons de populations cellulaires ont été obtenus par lavage des glandes mammaires, à des intervalles de 24 heures. On a utilisé la cytométrie de flux pour détecter les neutrophiles, les monocytes et les macrophages CD14+. Pendant l'induction de la lésion de glande mammaire (24 h), on a détecté seulement 1,2 % des neutrophiles CD14+ après traitement par le PBS et 1,3 % après traitement par le LPS. La résolution fut caractérisée par une augmentation de la proportion de neutrophiles CD14+. Jusqu'à 96 heures, après injection de PBS, à la différence de celle de LPS, la proportion de ne...

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“…The resolution of mammary gland injury was more rapid after PBS treatment than after LPS treatment. This result is consistent with our earlier findings [23,24].…”

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Sládek¹,

Rysanek²,

Faldyna

2002

Vet. Res.

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“…Sládek and Rys lánek [16] were the first to show that bovine PMN are phagocytosed by macrophages in the mammary gland of heifers. In contrast, necrosis can lead to an inflammatory reaction because of the release of the cellular content into the surrounding tissue.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis and necrosis of blood and milk polymorphonuclear leukocytes in early and midlactating healthy cows

et al. 2001

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-Increased milk somatic cell counts (SCC) are used as an indicator for bovine mastitis. During mastitis, polymorphonuclear leukocytes (PMN) become the predominant cell type. Shortly after parturition, the severity of mastitis is increased and several PMN functions are downregulated. Apoptotic and necrotic processes of PMN could influence SCC and PMN functions. In this study, the percentages of apoptotic and necrotic PMN in blood and milk from early and midlactating healthy cows were compared. Apoptosis and necrosis of PMN were quantified using a dual-color flow cytometric procedure with fluorescein labeled annexin-V (green) and propidium iodide (red). Using this technique three different subpopulations of bovine PMN could be detected: apoptotic cells (high intensive green fluorescence), necrotic cells (high intensive green and high intensive red fluorescence) and viable cells (low intensive green and low intensive red fluorescence). Following a 4 h incubation of blood from both groups of cows at 37°C to induce apoptosis, the mean percentage of apoptotic blood PMN was significantly higher (P < 0.01) in early lactating cows (15.1%, n = 9) compared with midlactating cows (5.3%, n = 10). The mean percentage of necrotic PMN remained lower than 5% in all cows. In contrast to blood, no significant difference was found between the percentage of apoptotic PMN in milk from early (41.2%, n = 7) and midlactating cows (34.0%, n = 8). The percentage of necrotic PMN in milk from early lactating cows (25.9%, n = 7) was significantly higher than that in midlactating cows (14.2%, n = 8) (P < 0.05). Higher percentages of apoptotic as well as necrotic PMN were consistently found in milk compared to blood in all cows. From these results, it can be concluded that spontaneously induced apoptosis was higher in blood PMN from early lactating cows than in blood PMN from midlactating cows. The higher percentage of necrotic milk PMN in early lactating cows than in midlactating cows could be explained by the induction of secondary necrosis. bovine neutrophil / apoptosis / necrosis / blood / milk Vet. Res. 32 (2001) 617-622 617

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The role of CD14 during resolution of experimentally induced Staphylococcus aureus and Streptococcus uberis mastitis

Sládek

Rysanek

2006

Comparative Immunology, Microbiology and Infectious Diseases

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Apoptosis of polymorphonuclear leukocytes of the juvenile bovine mammary gland during induced influx

Cited by 33 publications

References 28 publications

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Apoptosis and necrosis of blood and milk polymorphonuclear leukocytes in early and midlactating healthy cows

The role of CD14 during resolution of experimentally induced Staphylococcus aureus and Streptococcus uberis mastitis

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