Apoptotic Activity and bcl-2 Immunoreactivity in Meningiomas

Abramovich, Caroline M.; Prayson, Richard A.

doi:10.1309/ulmt-v1uc-d69h-mmqf

Cited by 30 publications

(22 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the importance of apoptosis in the development of central and peripheral nervous system, there are rather few reports concerning the magnitude of cell deletion in meningiomas [4][5][6][7].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Caspase-3 immunohistochemical expression is a marker of apoptosis, increased grade and early recurrence in intracranial meningiomas

et al. 2006

View full text Add to dashboard Cite

Caspase-3 is the ultimate executioner caspase that is essential for the nuclear changes associated with apoptosis. We investigated caspase-3 immunohistochemical expression in 58 primary intracranial meningiomas, using one monoclonal antibody detecting both precursor and cleaved caspase-3 (CPP32) and a second recognizing only the cleaved activated form (ASP175). Caspase-3 expression was analyzed in relation to baseline apoptosis-as illustrated by the expression of anti-single stranded DNA (ss-DNA), the antiapoptotic protein bcl-2, proliferation indices (Ki-67, PCNA, topoisomerase IIa, mitosin C), hormonal status (estrogen, progesterone, androgen receptors), standard clinicopathological parameters and patients' disease-free survival. Caspase-3 immunostaining was observed in 62% of cases for CPP32 and in 24% for ASP175. In both instances, the labeling index (LI) was significantly correlated with ss-DNA LI (p=0.038 and p=0.018). CPP32 but not ASP175 LI positively correlated with the mitotic index (p=0.001) and PCNA LI (p=0.004). Both CPP32 and ASP175 LIs were increased in nonbenign meningiomas (p<0.0001 and p=0.0035 respectively). In univariate and multivariate survival analyses, caspase-3 predicted meningioma recurrence, independently affecting disease-free survival (p=0.011 and p=0.047 respectively for CPP32; p<0.0001 and p=0.012 respectively for ASP175). Caspase-3 may prove to be a useful predictor of early recurrence in a group of neoplasms characterized by the frequent discordance between histology and clinical behavior.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Although the classical form of necrosis is a major histological factor influencing grading and prognosis in meningiomas [1][2][3], the occurrence and significance of apoptosis has been the subject of limited reports [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Caspase-3 immunohistochemical expression is a marker of apoptosis, increased grade and early recurrence in intracranial meningiomas

et al. 2006

View full text Add to dashboard Cite

show abstract

“…22 This is supported by the greater than 70% occurrence of protooncogene messenger RNA expression for c-myc and c-fos in meningiomas. Mutation in the tumor suppressor gene TP53 has been considered a reliable marker for malignant transformation of meningioma, 87 and the bcl-2 protooncogene is also correlated with higher-grade 1 Furthermore, expression of the ROS1 oncogene for tyrosine receptor kinase is common in meningiomas, which indicates that it may play a role in the origin of these tumors. 96 Although no single oncogene has been directly implicated in meningioma development, it is possible that either one or multiple oncogenes mentioned earlier will contribute to this process.…”

Section: Oncogene Expression In Meningiomasmentioning

confidence: 99%

Molecular genetics of meningiomas

Ragel¹,

Jensen²

2005

FOC

View full text Add to dashboard Cite

In this article the authors provide a brief description of the current understanding of meningioma genetics. Chromosome 22 abnormalities, especially in the Neurofibromatosis Type 2 (NF2) gene, have been associated with meningioma development. Loss of heterozygosity of chromosome 22 occurs in approximately 60% of meningiomas; however, loss of NF2 gene function occurs in only one third of these lesions. This discrepancy supports the theory that a second tumor suppressor gene exists on chromosome 22, and the authors introduce several possible gene candidates, including BAM22, LARGE, INI1, and MN1 genes. Deletions of 1p have also been shown to correlate with meningioma progression. The genetic similarities and differences among sporadic, NF2-associated, pediatric, and radiation-induced meningiomas are discussed, with the observation that the nonsporadic meningiomas have a higher incidence of multiple chromosomal abnormalities at presentation. Ultimately, a better understanding of the molecular pathways of meningioma tumorigenesis will lead to new, successful treatments.

show abstract

“…Interestingly, anaplastic meningiomas have been reported to exhibit a high apoptosis index, 4,5 and apoptosis is thought to be associated with atypical or malignant changes in these tumors. 30 The increased level of apoptosis in atypical and anaplastic meningiomas is associated with increased expression of the pro-apoptotic protein Bax and decreased expression of the anti-apoptotic protein Bcl2 in these cells.…”

Section: Discussionmentioning

confidence: 99%

“…3 Atypical and malignant meningiomas are histologically characterized by frequent mitoses, increased cellularity, high nuclear to cytoplasmic ratio, uninterrupted, patternless or sheet-like growth, and foci of necrosis. 2 In addition, anaplastic meningiomas exhibit an increased apoptosis index 4,5 and express higher levels of p53 and Bax and lower levels of Bcl2. 6,7 Fas-APO1 (CD95), is a 48-kd transmembrane cysteine-rich glycoprotein that belongs to the tumor necrosis factor receptor superfamily.…”

mentioning

confidence: 99%