Apoptotic and anti-angiogenic strategies in liver and gastrointestinal malignancies

Kountouras, Jannis; Zavos, Christos; Chatzopoulos, Dimitrios

doi:10.1002/jso.20254

Cited by 27 publications

(28 citation statements)

References 64 publications

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“…16,17 Our data displayed in this paper demonstrated that PDCD5, an apoptosisassociated protein, was significantly decreased in gastric tumors. Interestingly, the decreased PDCD5 expression was well correlated with the increased expression of Bcl-2 protein (p = 0.018).…”

Section: Discussionmentioning

confidence: 73%

Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells

Yang

Zhao

et al. 2006

Apoptosis

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The protein of programmed cell death 5 (PDCD5) is believed to participate in regulation of apoptosis. Although PDCD5 is reducibly expressed in various human tumors, it is not clear which expression level of PDCD5 is in gastric cancer (GC). In this study, we have systematically employed the approaches of RT-PCR, Real- time PCR, Immunohistochemistry (IHC), Immunofluorescence staining (IFS) and Western blot to determine the PDCD5 expression in GC cells and primary tumors, at mRNA and protein level, respectively. Our data revealed that the positive rate of PDCD5 expression in the gastric tumor tissues was significantly less than that of the normal tissues (14 out of 102 vs 36 out of 51), whereas, the decreased expression of PDCD5 protein was well correlated with the up-regulated expression of Bcl-2 in these tissues, and the up-regulated expression and nuclear translocation of PDCD5 protein were verified in the apoptotic GC cells induced by Diallyl trisulfide (DATS). Furthermore, the survival curve has suggested that the more PDCD5 expressions were found in the patients, the longer the survival periods were. Therefore, our observations lay down a reasonable postulation that PDCD5 may play a key role to regulate the apoptotic processes in the GC cells and gastric tumors.

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Section: Discussionmentioning

confidence: 73%

Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells

Yang

Zhao

et al. 2006

Apoptosis

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“…The inhibition of survivin has been demonstrated to be an effective molecular target therapy in the treatment of a variety of cancers, such as lung [38], breast [39], liver [40], pancreatic [41], oesophageal [42], colon [43] and prostatic [44] cancers. In recent years, molecular target therapy using survivin inhibition has been applied in the treatment of gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of siRNA targeting survivin in gastric cancer MGC-803 cells

Chen

Deng

2008

International Immunopharmacology

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“…Several reports have demonstrated survivin expression in the majority of human tumor types including lung, breast, colon, gastric, oesophageal, pancreatic, liver, bladder, uterine and ovarian cancers, leukaemias, neuroblastoma, brain tumors, pheochromocytoma, soft tissue sarcomas, melanomas and other skin cancers [38][39][40][41][42] .…”

Section: Discussionmentioning

confidence: 99%

Induction of apoptosis of human colon cancer cells by siRNA recombinant expression vector targeting survivin gene

Cai

Wang

Tao

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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This study constructed siRNA recombinant expression vector targeting survivin gene and observe the apoptosis induction effect of it in human colon cancer cells. siRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cells. The effect of siRNA recombinant expression vector was detected by RT-PCR, Western blot, MTT reduction assay and flow cytometry. It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting survivin gene was constructed successfully. Inhibition rate of survivin siRNA at mRNA and protein levels was 36.33% and 44.65% respectively. Growth of cancer cells was inhibited and the apoptosis rate was (17.24+/-2.13)%. The siRNA recombinant expression vector targeting survivin gene has been constructed successfully. It not only can inhibit the expression of survivin gene, but also can induce apoptosis in human colon cancer cells remarkably.

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Apoptotic and anti-angiogenic strategies in liver and gastrointestinal malignancies

Cited by 27 publications

References 64 publications

Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells

Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells

Inhibitory effect of siRNA targeting survivin in gastric cancer MGC-803 cells

Induction of apoptosis of human colon cancer cells by siRNA recombinant expression vector targeting survivin gene

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