Apoptotic and antiproliferative effects of Stigmast-5-en-3-ol from Dendronephthya gigantea on human leukemia HL-60 and human breast cancer MCF-7 cells

Fernando, Ilekuttige Priyan Shanura; Sanjeewa, K. K. Asanka; Ann, Yong-Seok; Ko, Chang-Ik; Lee, Seung‐Hong; Lee, Won Woo; Jeon, You‐Jin

doi:10.1016/j.tiv.2018.07.007

Cited by 31 publications

(19 citation statements)

References 27 publications

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“…The cleavage of PARP, a nuclear enzyme that maintains DNA stability, repair and transcription, is another crucial feature of apoptosis. Effector caspases cause the cleavage of PARP, thereby inhibiting its catalytic activity [9]. The present observations clarified the effect of UVB on the initiation of mitochondria-mediated apoptosis proteins and the dose-dependent attenuation effects of SHC4.…”

Section: Discussionsupporting

confidence: 61%

“…Initiator caspases, such as caspase-9, activate death receptors, whereas effector caspases directly mediate the cleavage of numerous cytoplasmic and nuclear substrates. Cytochrome c activates caspase-9 and subsequently activates downstream effector caspases such as caspase-3, −6 and −7 [9]. The cleavage of PARP, a nuclear enzyme that maintains DNA stability, repair and transcription, is another crucial feature of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Cells were stained with either Hoechst 33342 or mixture of acridine orange and ethidium bromide according to the method described in our previous publication [9]. Nuclear morphologies were visualized on an EVOS FL Auto 2 Imaging microscope (Thermo-Fisher, Waltham, MA, USA).…”

Section: Analysis Of Nuclear Morphologymentioning

confidence: 99%

“…DNA damage in individual cells was evaluated by alkaline comet assay following the procedure outlined in our previous publication [9]. Briefly, cells were harvested and suspended in low-melting agarose at 37 • C and gently applied on the surface of agarose coated slides.…”

Section: Comet Assaymentioning

confidence: 99%

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Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

et al. 2020

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Ultraviolet B (UVB) radiation-induced oxidative skin cell damage is a major cause of photoaging. In the present study, a low molecular weight fucoidan fraction (SHC4) was obtained from Sargassum horneri by Celluclast-assisted extraction, followed by step gradient ethanol precipitation. The protective effect of SHC4 was investigated in human keratinocytes against UVB-induced oxidative stress. The purified fucoidan was characterized by Fourier-transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (NMR), agarose gel-based molecular weight analysis and monosaccharide composition analysis. SHC4 had a mean molecular weight of 60 kDa, with 37.43% fucose and 28.01 ± 0.50% sulfate content. The structure was mainly composed of α-L-Fucp-(1→4) linked fucose units. SHC4 treatment dose-dependently reduced intracellular reactive oxygen species (ROS) levels and increased the cell viability of UVB exposed HaCaT keratinocytes. Moreover, SHC4 dose-dependently inhibited UVB-induced apoptotic body formation, sub-G1 accumulation of cells and DNA damage. Inhibition of apoptosis was mediated via the mitochondria-mediated pathway, re-establishing the loss of mitochondrial membrane potential. The UVB protective effect of SHC4 was facilitated by enhancing intracellular antioxidant defense via nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Further studies may promote the use of SHC4 as an active ingredient in cosmetics and nutricosmetics.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Nuclear Morphologymentioning

confidence: 99%

Section: Comet Assaymentioning

confidence: 99%

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Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

et al. 2020

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“…Stigmast-5-en-3-ol is the second most abundant compound in both BEA (22.52%) and CEA (3.60%). The apoptotic and antiproliferative effects against human leukemia HL-60 and MCF-7 cells were also demonstrated [41].…”

Section: Discussionmentioning

confidence: 94%

Potential of Thai Herbal Extracts on Lung Cancer Treatment by Inducing Apoptosis and Synergizing Chemotherapy

et al. 2020

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The incidence of lung cancer has increased while the mortality rate has continued to remain high. Effective treatment of this disease is the key to survival. Therefore, this study is a necessity in continuing research into new effective treatments. In this study we determined the effects of three different Thai herbs on lung cancer. Bridelia ovata, Croton oblongifolius, and Erythrophleum succirubrum were extracted by ethyl acetate and 50% ethanol. The cytotoxicity was tested with A549 lung cancer cell line. We found four effective extracts that exhibited toxic effects on A549 cells. These extracts included ethyl acetate extracts of B. ovata (BEA), C. oblongifolius (CEA), and E. succirubrum (EEA), and an ethanolic extract of E. succirubrum (EE). Moreover, these effective extracts were tested in combination with chemotherapeutic drugs. An effective synergism of these treatments was found specifically through a combination of BEA with methotrexate, EE with methotrexate, and EE with etoposide. Apoptotic cell death was induced in A549 cells by these effective extracts via the mitochondria-mediated pathway. Additionally, we established primary lung cancer and normal epithelial cells from lung tissue of lung cancer patients. The cytotoxicity results showed that EE had significant potential to be used for lung cancer treatment. In conclusion, the four effective extracts possessed anticancer effects on lung cancer. The most effective extract was found to be E. succirubrum (EE).

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Heliotropium curassavicum extract: Potential therapeutic agent for liver cancer through cytotoxicity, apoptosis, and molecular docking analysis

Abutaha,

Alghamdi,

Alshahrani

et al. 2024

Arabian Journal of Chemistry

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Apoptotic and antiproliferative effects of Stigmast-5-en-3-ol from Dendronephthya gigantea on human leukemia HL-60 and human breast cancer MCF-7 cells

Cited by 31 publications

References 27 publications

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Potential of Thai Herbal Extracts on Lung Cancer Treatment by Inducing Apoptosis and Synergizing Chemotherapy

Heliotropium curassavicum extract: Potential therapeutic agent for liver cancer through cytotoxicity, apoptosis, and molecular docking analysis

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