Apoptotic Death of Cancer Stem Cells for Cancer Therapy

He, Yingchun; Zhou, Fangliang; Shen, Yi; Liao, Duan‐Fang; Cao, Deliang

doi:10.3390/ijms15058335

Cited by 54 publications

(50 citation statements)

References 118 publications

(126 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…33 Recently, He et al reviewed that Rg3 is a natural inhibitor of colon cancer stem cell proliferation by inducing apoptosis through caspase-9 and caspase-3 pathways. 34 Weber et al also stated that Rg3 is thought to inhibit the proliferation of cancer stem cells in brain glioma. 35 Thus, it is conceivable that Rg3-elevated f-RGE used in our study showed an antiproliferative effect on cancer stem-like cells.…”

Section: F-rge Decreased Cancer Stem-like Cell Viabilitymentioning

confidence: 97%

Fermented Red Ginseng Extract Inhibits Cancer Cell Proliferation and Viability

Jeon

Lee

et al. 2015

Journal of Medicinal Food

View full text Add to dashboard Cite

Red ginseng (Panax ginseng C.A. Meyer) is the most widely recognized medicinal herb due to its remedial effects in various disorders, such as cancers, diabetes, and heart problems. In this study, we investigated the anticancer effect of fermented red ginseng extract (f-RGE; provided by Jeonju Biomaterials Institute, Jeonju, South Korea) in a parallel comparison with the effect of nonfermented red ginseng extract (nf-RGE; control) on several cancer cell lines--MCF-7 breast cancer cells, HepG2 hepatocellular carcinoma cells, and reprogrammed MCF-7 cells (mimicking cancer stem cells). Cells were cultured at various concentrations of RGE (from 0.5 up to 5 mg/mL) and their viabilities and proliferative properties were examined. Our data demonstrate the following: (1) nf-RGE inhibited cell viability at ≥1 mg/mL for MCF-7 cells and ≥2 mg/mL for HepG2 cells, (2) in the presence of a carcinogenic agent, 12-O-tetradecanoylphorbol-13-acetate (TPA), nf-RGE treatment in combination with paclitaxel synergistically decreased MCF-7 as well as HepG2 cell viability, (3) f-RGE (which contained a greater level of Rg3 content) more effectively decreased the viability of MCF-7 and HepG2 cells compared to nf-RGE, and (4) f-RGE appeared more potent for inhibiting cancerous differentiation of reprogrammed MCF-7 cells in a synergistic fashion with paclitaxel, especially in the presence of TPA, compared to nf-RGE. These findings suggest that f-RGE treatment may be more effective for decreasing cancer cell survival by inducing apoptotic cell death and also presumably for preventing cancer stem cell differentiation compared to nf-RGE.

show abstract

Section: F-rge Decreased Cancer Stem-like Cell Viabilitymentioning

confidence: 97%

Fermented Red Ginseng Extract Inhibits Cancer Cell Proliferation and Viability

Jeon

Lee

et al. 2015

Journal of Medicinal Food

View full text Add to dashboard Cite

show abstract

“…The failure that occur throughout this process can lead to tumor development [11] . Apoptotic signaling pathways are deregulated in lung CSCs CSCs express high levels of antiapoptosis proteins [12] . Flavopiridol can induce cytotoxic effects by cell cycle arrest at G1 and G2 phases though inhibition of Cdk2, Cdk4, and Cdk1 and induction of apoptosis in various human cancers.…”

Section: Introductionmentioning

confidence: 99%

Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells

et al. 2016

View full text Add to dashboard Cite

Background:Flavopiridol a semisynthetic flavone that inhibits cyclin-dependent kinases (CDKs) and has growth-inhibitory activity and induces a blockade of cell-cycle progression at G1-phase and apoptosis in numerous human tumor cell lines and is currently under investigation in phase II clinical trials. Cancer stem cells (CSCs) are comprised of subpopulation of cells in tumors that have been proposed to be responsible for recurrence and resistance to chemotherapy. The aim of the present study was to investigate the effects of flavopiridol in cancer stem cell cytoskeleton, cell adhesion, and epithelial to mesenchymal transition in CSCs.Methods:The cells were treated with flavopiridol to determine the inhibitory effect. Cell viability and proliferation were determined by using the WST-1 assay. Caspase activity and immunofluorescence analyses were performed for the evaluation of apoptosis, cell cytoskeleton, and epithelial-mesenchymal transition (EMT) markers. The effects of flavopiridol on the cell cycle were also evaluated. Flow cytometric analysis was used to detect the percentages of CSCs subpopulation. We analyzed the gene expression patterns to predict cell cycle and cell cytoskeleton in CSCs by RT-PCR.Results:Flavopiridol-induced cytotoxicity and apoptosis at the IC50 dose, resulting in a significant increase expression of caspases activity. Cell cycle analyses revealed that flavopiridol induces G1 phase cell cycle arrest. Flavopiridol significantly decreased the mRNA expressions of the genes that regulate the cell cytoskeleton and cell cycle components and cell motility in CSCs.Conclusion:Our results suggest that Flavopiridol has activity against lung CSCs and may be effective chemotherapeutic molecule for lung cancer treatment.

show abstract

“…The main markers used for isolation, identification and purification of CSCs include surface cell-adhesion molecules (e.g., CD133, CD24, hyaluronic acid (HA) receptor CD44, CD44, CD166, etc. ), cytoprotective enzymes (such as aldehyde dehydrogenase, ALDH), transcription factors (e.g., OCT-4, SOX-2), and drug-efflux pumps (e.g., ATP-binding cassette (ABC) drug transporters (ABCG2, ABCB5), multidrug resistance transporter1, MDR1), EpCAM, CXCR4, Nestin and LRCs [28][29][30]. Telomerase are also applied for identification of CSCs.…”

Section: Stem Cell Markersmentioning

confidence: 99%

Stem Cells and Cancer

Ali¹

2016

J Cell Sci Ther

View full text Add to dashboard Cite

Stem cells are undifferentiated cells that can differentiate into specialized cell and can divide to produce more stem cells. The concept of cancer stem cells has been discussed in the scientific literature since the 19th century. Circumstantial evidence suggests that most tumors are heterogeneous and contain a small population of cancer stem cells that exhibit distinctive self-renewal, proliferation and differentiation capabilities, which are believed to play a crucial role in tumor progression, drug resistance, recurrence and metastasis in multiple malignancies. Unlike normal adult stem cells that remain constant in number, cancer stem cells can increase in number as tumors grow, and give rise to progeny that can be both locally invasive and colonize distant sites-the two hallmarks of malignancy. Rapid advances in the cancer stem cell field have provided cause for optimism for the development of more reliable cancer therapies in the future. Strategies aimed at efficient targeting of cancer stem cells are becoming important for monitoring the progress of cancer therapy and for evaluating new therapeutic approaches.

show abstract

Apoptotic Death of Cancer Stem Cells for Cancer Therapy

Cited by 54 publications

References 118 publications

Fermented Red Ginseng Extract Inhibits Cancer Cell Proliferation and Viability

Fermented Red Ginseng Extract Inhibits Cancer Cell Proliferation and Viability

Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells

Stem Cells and Cancer

Contact Info

Product

Resources

About