Apoptotic effects of acacetin in human colon cancer HT-29 and HCT 116 cells

Abstract

Aim: Acacetin is a natural flavone compound, which is found in several plants as Robinia pseudoacacia and is demonstrated to have anticancerogenic activities in many types of cancer (e.g., human nonsmall cell lung cancer, and prostate). Colorectal carcinoma (CRC) is one of the serious health problems and is a complex disease. We intended to find a more effective new candidate for the treatment of colon cancer, and hence, we designed this study to investigate the effects of acacetin on CRC (HT-29, HCT 116) in vitro. Methods: The study was carried out with the methods that determine for apoptosis (WST-1, Caspase 3/BCA, Annexin V). Results: Acacetin showed antitumor and apoptosis-inducing effects in the CRC cell lines. Conclusions: Acacetin was effective on CRC cell lines, besides no lethal effect on healthy lung cells (MRC-5).

All‐trans retinoic acid exhibits anti‐proliferative and differentiating activity in Merkel cell carcinoma cells via retinoid pathway modulation

“…MCCN cells MCC13 and MCC26, and MCCP cells PeTa and WaGa and engineered‐GFP (eGFP) PeTa‐GFP and WaGa‐GFP, 23 were cultured in RPMI 1640 medium with 10% FBS (EuroClone, Milan, Italy) 24 . Control human fibroblast cells MRC‐5 were cultured in DMEM/F‐12 medium with 10% FBS (EuroClone, Milan, Italy) 25 . ATRA (Sigma‐Aldrich, Milan, Italy) was dissolved in DMSO at a concentration of 100 mM 8,13,14,26 .…”

“…24 Control human fibroblast cells MRC-5 were cultured in DMEM/F-12 medium with 10% FBS (EuroClone, Milan, Italy). 25 ATRA (Sigma-Aldrich, Milan, Italy) was dissolved in DMSO at a concentration of 100 mM . 8,13,14,26 Cell proliferation was tested with WST-1 assay (Merck, Milan, Italy).…”