Apoptotic Effects on HL60 Human Leukaemia Cells Induced by Lavandin Essential Oil Treatment

Abstract

Recent scientific investigations have reported a number of essential oils to interfere with intracellular signalling pathways and to induce apoptosis in different cancer cell types. In this paper, Lavandin Essential Oil (LEO), a natural sterile hybrid obtained by cross-breeding L. angustifolia × L. latifolia, was tested on human leukaemia cells (HL60). Based on the MTT results, the reduced cell viability of HL60 cells was further investigated to determine whether cell death was related to the apoptotic process. HL60 cells treated for 24 h with LEO were processed by flow cytometry, and the presence of Annexin V was measured. The activation of caspases-3 was evaluated by western blot and immunofluorescence techniques. Treated cells were also examined by scanning and transmission electron microscopy to establish the possible occurrence of morphological alterations during the apoptotic process. LEO main compounds, such as linalool, linalyl acetate, 1,8-cineole, and terpinen-4-ol, were also investigated by MTT and flow cytometry analysis. The set of obtained results showed that LEO treatments induced apoptosis in a dose-dependent, but not time-dependent, manner on HL60 cells, while among LEO main compounds, both terpinen-4-ol and linalyl acetate were able to induce apoptosis.

Headspace/GC–MS Analysis and Investigation of Antibacterial, Antioxidant and Cytotoxic Activity of Essential Oils and Hydrolates from Rosmarinus officinalis L. and Lavandula angustifolia Miller

“…After 24 h, 48 h and 72 h of treatment, the medium was removed, MTT solution (0.5 mg/mL) was added to the cells and they were incubated for 3 h in dark conditions at 37 • C. DMSO was used to solubilize the formazan crystals and the absorbance was read by Tecan Sunrise™ (Tecan Group Ltd., Männedorf, Switzerland) UV-vis spectrophotometer at 595 nm. The obtained optical density values were converted into percentage of cell viability and the data were elaborated with AAT Bioquest EC 50 Calculator (Sunnyvale, CA, USA) [19] in order to obtain the concentration at which the investigated substance exerts half of its maximal response values (EC 50 ). The values were repeated three times and reported as mean ± SD.…”

“…L. angustifolia EO is used in perfumery and cosmetics and its activity on the central nervous system, as a sedative, anxiolytic and antidepressant, was also evidenced [12][13][14]. Furthermore, biological activities of EOs from Lavandula genus, such as antifungal, antibacterial, antioxidant and anticancer effects, were reported [15][16][17][18][19][20]. EO chemical composition is highly complex and it can vary considerably depending on several factors, such as the cultivation area, environmental conditions, morphological characteristics and processing techniques of the plant [21][22][23]; moreover, the chemical composition influences the way in which EOs exert their antibacterial activity [24].…”

Detection of Volatiles by HS-SPME-GC/MS and Biological Effect Evaluation of Buddha’s Hand Fruit

“…Concerning Hy cytotoxicity, EC 50 results were quite low, which revealed a very good activity. In our investigation, Hy was rich in α -terpineol and terpinen-4-ol, whose potential anticancer action, through cycle inhibition and apoptosis induction, has been demonstrated [ 67 , 68 ]. These compounds could explain the Hy cytotoxicity that has, until now, never been investigated.…”

LRIG2 regulates cell proliferation, migration and apoptosis of osteosarcoma

“…Apoptosis, also called programmed cell death, is a mechanism for the removal of unwanted and damaged cells in the maintenance of normal tissue homeostasis [ 23 – 25 ]. The resistance to apoptosis will cause tumorigenesis and results in cancer cells resistance to chemotherapy and radiotherapy [ 26 – 29 ]. In line with previous studies, our study showed that silencing LRIG2 enhanced the apoptosis of OS cell, due to the pro-apoptotic proteins increased and anti-apoptotic proteins decreased.…”