Apoptotic index by Annexin V flow cytometry: Adjunct to morphologic and cytogenetic diagnosis of myelodysplastic syndromes

Merchant, Shakil H.; Gonchoroff, Nick J.; Hutchison, Robert

doi:10.1002/1097-0320(20010215)46:1<28::aid-cyto1034>3.0.co;2-h

Cited by 41 publications

(31 citation statements)

References 14 publications

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“…Although the cell division cycle and apoptosis might appear to be quite different from a physiological perspective, recent evidence suggests that they are intertwined, and that an enzyme in one system might also have an important role in the other system. [21,22] The degree of apoptosis in our study is relatively lower than previously reported with annexin V staining [15,23,24]. Similar were the findings of our previous study in a smaller group of patients [14].…”

Section: Discussionsupporting

confidence: 85%

“…This allows for live cells (unstained with either fluorochrome) to be discriminated from apoptotic cells (stained only with annexin V) and necrotic cells (stained with both annexin and PI). The apoptotic index was calculated as the percentage of annexin-V-positive-and PI-negative cells divided by the total number of cells in the gated region [15]. Analysis of cell cycle using flow cytometry…”

Section: Annexin V Stainingmentioning

confidence: 99%

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Cell cycle and apoptosis regulatory gene expression in the bone marrow of patients with de novo myelodysplastic syndromes (MDS)

et al. 2009

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Section: Discussionsupporting

confidence: 85%

Section: Annexin V Stainingmentioning

confidence: 99%

Cell cycle and apoptosis regulatory gene expression in the bone marrow of patients with de novo myelodysplastic syndromes (MDS)

et al. 2009

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“…To determine the role of PCD in heat-induced bleaching, we assessed the relationship between temperature elevation and cell death activity using cell death index [17][18][19][20][21][22][23][24] in the symbiotic anemone Aiptasia sp. This species was used as it has an identical symbiosis to reef corals, but is easier to culture and microscopy does not require decalcification.…”

Section: Introductionmentioning

confidence: 99%

Heat stress induces different forms of cell death in sea anemones and their endosymbiotic algae depending on temperature and duration

et al. 2004

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Bleaching of reef building corals and other symbiotic cnidarians due to the loss of their dinoflagellate algal symbionts ( ¼ zooxanthellae), and/or their photosynthetic pigments, is a common sign of environmental stress. Mass bleaching events are becoming an increasingly important cause of mortality and reef degradation on a global scale, linked by many to global climate change. However, the cellular mechanisms of stress-induced bleaching remain largely unresolved. In this study, the frequency of apoptosislike and necrosis-like cell death was determined in the symbiotic sea anemone Aiptasia sp. using criteria that had previously been validated for this symbiosis as indicators of programmed cell death (PCD) and necrosis. Results indicate that PCD and necrosis occur simultaneously in both host tissues and zooxanthellae subject to environmentally relevant doses of heat stress. Frequency of PCD in the anemone endoderm increased within minutes of treatment. Peak rates of apoptosis-like cell death in the host were coincident with the timing of loss of zooxanthellae during bleaching. The proportion of apoptosis-like host cells subsequently declined while cell necrosis increased. In the zooxanthellae, both apoptosis-like and necrosis-like activity increased throughout the duration of the experiment (6 days), dependent on temperature dose. A stress-mediated PCD pathway is an important part of the thermal stress response in the sea anemone symbiosis and this study suggests that PCD may play different roles in different components of the symbiosis during bleaching.

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“…[7][8][9][10] The frequency and percentage of cells showing apoptosis in these patients vary depending on the methodology used and cell population analyzed. Apoptotic cell death can be measured by various methods including terminal dUTP nick-end labeling (TUNEL), caspase, annexin V and mitochondrial membrane potential.…”

Section: Discussionmentioning

confidence: 99%

“…There is a considerable body of data suggesting that MDS is distinguished from AML by a higher degree of apoptosis. [3][4][5][6][7] While a number of studies have shown the difference in apoptosis between MDS and AML, only a few have specifically compared RAEB-T with other categories of MDS and AML, producing conflicting results. [8][9][10] Therefore, in the present study, we compared the degree of apoptosis in RAEB-T with that in refractory anemia (RA), with ringed sideroblasts (RARS), RAEB, and AML using the Annexin V 11 and mitochondrial potential assays.…”

Section: Introductionmentioning

confidence: 99%

More cell death in refractory anemia with excess blasts in transformation than in acute myeloid leukemia

et al. 2002

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Refractory anemia with excess blasts in transformation (RAEB-T) is a subgroup of myelodysplastic syndrome (MDS) in which the bone marrow blast count ranges from 20% to 30%. The recently proposed World Health Organization Classification of Hematologic Malignancies eliminated this category from MDS by lowering the blast count cutoff for acute myeloid leukemia (AML) from 30% to 20%. However, MDS is distinguished from AML by a significant increase in apoptosis. To investigate the difference in apoptosis between RAEB-T, AML, and other categories of MDS, we prospectively analyzed fresh bone marrow samples using the Annexin V and mitochondrial potential assays. There was a significantly higher level of apoptosis in RAEB-T than in AML according to both assays, while no significant differences between RAEB-T and other categories of MDS were noted. The data suggest that RAEB-T is more likely to be an advanced stage of MDS and biologically different from AML.

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Apoptotic index by Annexin V flow cytometry: Adjunct to morphologic and cytogenetic diagnosis of myelodysplastic syndromes

Cited by 41 publications

References 14 publications

Cell cycle and apoptosis regulatory gene expression in the bone marrow of patients with de novo myelodysplastic syndromes (MDS)

Cell cycle and apoptosis regulatory gene expression in the bone marrow of patients with de novo myelodysplastic syndromes (MDS)

Heat stress induces different forms of cell death in sea anemones and their endosymbiotic algae depending on temperature and duration

More cell death in refractory anemia with excess blasts in transformation than in acute myeloid leukemia

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