Apoptotic signaling proteins: possible participation in the regulation of vasopressin and catecholamines biosynthesis in the hypothalamus

Chernigovskaya, E. V.; Taranukhin, A. G.; Глазова, М. В.; Yamova, L. A.; Fedorov, Lev M.

doi:10.1007/s00418-005-0016-x

Cited by 22 publications

(14 citation statements)

References 37 publications

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“…It can activate mitochondrial caspases, which cleave MAP kinases (activators of CDC2 kinases and cell cycle at G2/M phase; Marchetti et al 2004) and induce apoptosis-related events (Braithwaite et al 2006, Chowdhury et al 2006. When concerned with the role of p53 in controlling secretory activity, it is able to suppress growth factor secretion (VEGF, Hassan et al 2006) and insulin-like growth factor binding proteins (Grimberg et al 2006), and to promote vasopressin and catecholamine secretion (Chernigovskaya et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH

Sirotkin¹,

Benco²,

Tandlmajerová³

et al. 2008

Reproduction

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The aim of our in vitro experiments was to examine the role of transcription factor p53 in controlling the basic functions of ovarian cells and their response to hormonal treatments. Porcine ovarian granulosa cells, transfected and non-transfected with a gene construct encoding p53, were cultured with ghrelin and FSH (all at concentrations of 0, 1, 10, or 100 ng/ml). Accumulation of p53, of apoptosisrelated (MAP3K5) and proliferation-related (cyclin B1) substances was evaluated by immunocytochemistry. The secretion of progesterone (P 4 ), oxytocin (OT), prostaglandin F (PGF), and E (PGE) was measured by RIA. Transfection with the p53 gene construct promoted accumulation of this transcription factor within cells. It also stimulated the expression of a marker of apoptosis (MAP3K5). Over-expression of p53 resulted in reduced accumulation of a marker of proliferation (cyclin B1), P 4 , and PGF secretion and increased OT and PGE secretion. Ghrelin, when added alone, did not affect p53 or P 4 , but reduced MAP3K5 and increased PGF and PGE secretion. Over-expression of p53 reversed the effect of ghrelin on OT, caused it to be inhibitory to P 4 secretion, but did not modify its action on MAP3K5, PGF, or PGE. FSH promoted the accumulation of p53, MAP3K5, and cyclin B1; these effects were unaffected by p53 transfection. These multiple effects of the p53 gene construct on luteinizing granulosa cells, cultured with and without hormones 1) demonstrate the effects of ghrelin and FSH on porcine ovarian cell apoptosis and secretory activity, 2) confirm the involvement of p53 in promoting apoptosis and inhibiting P 4 secretion in these cells, 3) provide the first evidence that p53 suppress proliferation of ovarian cells, 4) provide the first evidence that p53 is involved in the control of ovarian peptide hormone (OT) and prostaglandin (PGF and PGE) secretion, and 5) suggest that p53 can modulate, but probably not mediate, the effects of ghrelin and FSH on the ovary. Reproduction (2008) 136 611-618

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Section: Introductionmentioning

confidence: 99%

Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH

Sirotkin¹,

Benco²,

Tandlmajerová³

et al. 2008

Reproduction

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“…Thus, p53 plays an important functional role in the regulation of cell processes that are not connected with apoptosis. Previously, we had demonstrated that water deprivation leads to simultaneous activation of synthesis of VP and p53 in the mouse brain [6]. Moreover, p53 deficiency led to reduced expression of VP in the hypothalamic centers [6].…”

Section: Discussionmentioning

confidence: 98%

“…Dehydration, well known stimuli for vasopressin (VP) activation, also resulted in the increased expression of p53 protein in hypothalamic neurons [4,6]. Conversely, in p53-deficient mice the content of VP was significantly decreased in magnocellular neurons of the PVN and supraoptical nucleus (SON) of the hypothalamus [6], suggesting that p53 can participate in the regulation of VP expression.…”

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confidence: 99%

Effect of p53 Inhibition by Pifithrin-Alpha on Functional Activity of Vasopressin Neurons in Rat Hypothalamus

Дорофеева

Chernigovskaya

Nikitina

et al. 2015

Neurosci Behav Physi

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Introduction. The transcription factor and tumor suppressor p53 participates in the regulation of transcription, cell cycle, apoptosis, as well as differentiation and cellular lifespan [11,27,40]. The wide range of reports made on p53 knockout animals demonstrates the differential effects of p53 deficiency. It was shown that part of p53 knockout embryos die during development, other surviving mice develop exencephaly, but mainly the p53 knockout mice develop normally [15,33]. However, Armstrong and coauthors mentioned increased neuronal apoptosis in the spinal cord of p53 knockout mice [1], which can mean another role of p53 under certain conditions [33]. Such differential effects of p53 deficiency and, on the other hands, high percentages of surviving animals suggests that p53 dependent effects in neural development and neural differentiation can be compensated by the other members of the p53 family or other regulatory mechanisms [15].Accumulating data support processes other than apoptosis in which p53 can participate, such as the regulation of neuronal proliferation and differentiation, the control of axonal outgrowth, and even, regeneration after nerve injury [31,33]. Expression of p53 was demonstrated in different parts of the central and peripheral nervous systems such as the cerebellum, hindbrain, hypothalamus, thalamus, the cortex, and spinal cord [34]. In hippocampal neurons, p53 was also detected in synapses where its content and phosphorylation level were increased after exposure of the cells to the DNA-damaging agent, and such increased levels of p53 correlated with dramatically decrease of synapsin I immunoreactivity in the cell terminals [9]. Immobilization stress, which is not associated with direct cellular damage, leads to increased expression of p53 mRNA in the hippocampus and In the present work, we investigated the effects of p53 inhibition by pifithrin-alpha (PFT) in vitro and in vivo on functioning vasopressinergic magnocellular neurons of rat hypothalamus. In vivo treatments with PFT were done by intrahypothalamic microinjections or by intraperitoneal injections. In in vitro experiments hypothalamic slices containing supraoptic nuclei and intact pituitary were incubated with or without PFT. In all experiments, we observed accumulation of vasopressin (VP) in the cell perikarya after PFT injections; however, expression of VP mRNA was not changed. Analysis of VP content in the posterior pituitary demonstrated that the amount of VP was significantly decreased after PFT treatments. Additionally, long-term inhibition of p53 in experiments with intrahypothalamic injections of PFT resulted in an increased diuresis rate. The obtained results demonstrated that in all experiments PFT treatments inhibited VP anterograde transport from the cells of supraoptic nuclei. Moreover, analysis of MEK/ERK activities revealed that phosphorylation levels of MEK1/2 and ERK1/2 were decreased after PFT treatments. Our findings provide new evidence that p53 could be involved in the control of VP secretion from the hyp...

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“…By reducing the gC1qR mitochondrial protein with an iRNA approach, the antiapoptotic property of the protein in the germ cells could be demonstrated. Chernigovskaya et al (2005) investigated the possible relationship between the functional activity of neurosecretory cells of the mouse hypothalamus and apoptosis related proteins. Their data suggest that p53, p21 (Waf1/Cip1) and Bcl-2 proteins, besides aVecting cell cycle, tumor suppression and apoptosis, may act as modulators of neurosecretory activity of hypothalamic neurons.…”

Section: Programmed Cell Death In Its Various Formsmentioning

confidence: 99%

The histochemistry and cell biology vade mecum: a review of 2005–2006

Taatjes

Zuber

Roth

2006

Histochem Cell Biol

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The procurement of new knowledge and understanding in the ever expanding discipline of cell biology continues to advance at a breakneck pace. The progress in discerning the physiology of cells and tissues in health and disease has been driven to a large extent by the continued development of new probes and imaging techniques. The recent introduction of semi-conductor quantum dots as stable, specific markers for both fluorescence light microscopy and electron microscopy, as well as a virtual treasure-trove of new fluorescent proteins, has in conjunction with newly introduced spectral imaging systems, opened vistas into the seemingly unlimited possibilities for experimental design. Although it oftentimes proves difficult to predict what the future will hold with respect to advances in disciplines such as cell biology and histochemistry, it is facile to look back on what has already occurred. In this spirit, this review will highlight some advancements made in these areas in the past 2 years.

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Apoptotic signaling proteins: possible participation in the regulation of vasopressin and catecholamines biosynthesis in the hypothalamus

Cited by 22 publications

References 37 publications

Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH

Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH

Effect of p53 Inhibition by Pifithrin-Alpha on Functional Activity of Vasopressin Neurons in Rat Hypothalamus

The histochemistry and cell biology vade mecum: a review of 2005–2006

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