Apparatus &amp; Experimental Design for Measuring Fermentation Rates in Yeast

Tatina, Robert

doi:10.2307/4448836

Cited by 10 publications

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“…A variety of setups have been devised to study yeast fermentation. Some involve the use of test tubes (Taylor, 1987;Johnson, 1998;Bartlett, 2002), others the use of burettes and pipettes (Yurkiewicz et al, 1989) and/or Erlenmeyer flasks (McElroy, 1976;Tatina, 1989). Still others make use of materials that are common in the contemporary molecular biology laboratory (e.g., 15 mL centrifuge tubes and Eppendorf tubes; Reinking et al, 1994;Grammer, 2012).…”

Section: A Simple Microscale Setupmentioning

confidence: 99%

A Simple Microscale Setup for Investigating Yeast Fermentation in High School Biology Classrooms

Chan

2016

The American Biology Teacher

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High school students often find the concept of respiration difficult. Yeast, a readily available resource, offers promising material for studying the topic. This article describes a low-cost, microscale setup for investigating yeast fermentation. The observations in the practical activity are visually appealing to learners. The article also illustrates how this setup can be used to promote student engagement with scientific ideas by prompting students to (1) predict what they will observe in the activity and (2) link what they actually observe in it to the underlying scientific ideas, in the context of studying the effects of different sugar substrates on yeast fermentation. The simple setup can be easily modified for various scientific investigations related to yeast fermentation and, hence, represents a promising teaching tool for teaching this difficult-to-learn topic in high school biology classrooms.

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Section: A Simple Microscale Setupmentioning

confidence: 99%

A Simple Microscale Setup for Investigating Yeast Fermentation in High School Biology Classrooms

Chan

2016

The American Biology Teacher

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“…Although the kinetics of fermentation may be monitored by measuring the disappearance of glucose or the appearance of ethanol, the most expeditious method is to measure the volume of carbon dioxide (CO 2 ) produced by the reaction. Many methods for measuring the amount of evolved CO 2 have been reported, including the following: balloon inflation; counting gas bubbles; liquid displacement from inverted graduated cylinders, pipettes, test tubes, , capillary tubes, or microfuge tubes; and use of electronic sensors. , However, each of these approaches has practical limitations: some yield only semiquantitative data, whereas others have limited accuracy or require expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

Measuring Yeast Fermentation Kinetics with a Homemade Water Displacement Volumetric Gasometer

Weinberg

2018

J. Chem. Educ.

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A simple homemade water displacement volumetric gasometer constructed from plastic water bottles, plastic tubing, and a graduated cylinder was used to measure the evolution of carbon dioxide by the fermentation of sugars by baker’s yeast. The apparatus provides reproducible, quantitative data that can be graphed to display phenomena such as induction lag time, steady-state growth, and nutrient depletion, and can be used to demonstrate the effect of a variety of conditions (such as carbohydrate type and concentration, inhibitors, pH, and temperature) on yeast fermentation kinetics. Advanced students can use the gasometer to calculate kinetic parameters such as the Michaelis constant, maximal velocity, and activation energy. The gasometer can be a versatile tool for exploring the principles of fermentation and enzyme kinetics in science laboratories ranging from elementary school to high school advanced placement chemistry and first-year college chemistry.

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Quantitation & Case-Study-Driven Inquiry to Enhance Yeast Fermentation Studies

Grammer

2012

The American Biology Teacher

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We propose a procedure for the assay of fermentation in yeast in microcentrifuge tubes that is simple and rapid, permitting assay replicates, descriptive statistics, and the preparation of line graphs that indicate reproducibility. Using regression and simple derivatives to determine initial velocities, we suggest methods to compare the effects of experimental variables. This technique is straightforward enough to facilitate design of an inquiry lab based on a scenario that explores modifications to enhance the rate of fermentation.

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Apparatus & Experimental Design for Measuring Fermentation Rates in Yeast

Cited by 10 publications

References 0 publications

A Simple Microscale Setup for Investigating Yeast Fermentation in High School Biology Classrooms

A Simple Microscale Setup for Investigating Yeast Fermentation in High School Biology Classrooms

Measuring Yeast Fermentation Kinetics with a Homemade Water Displacement Volumetric Gasometer

Quantitation & Case-Study-Driven Inquiry to Enhance Yeast Fermentation Studies

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